From a behavioral point of view, human crews into Space will have to both live and work in physical environment (microgravity, 1/3 g, 1/6 g), confined environment (spatial restriction, social constraints, and sensorial privation), and isolated environment (familiar privation, cultural background, and remote communication) that involve a multisystem adaptive model on a long-duration process. Physiological, medical, psychological, sociological, anthropological, and ethological impacts have been emphasized in a wide panel of investigations. The current results are presented with a focus on relevant methods in ethology based on the observation, description, and quantification of (i) the individual behavior from short-term orbital missions; (ii) the social behavior during inter-planetary missions simulated in terrestrial environments; and (iii) the cultural behavior in considering manned missions on Moon, on Mars, and beyond. Global analysis highlights that the crewmembers going into Space will be definitively interactive men and women with personal experiences, social rules, and new cultural habits. They will have their individual identities and they will be a group entity for extended periods of time.