Wiring of PQQ–dehydrogenases

Laurinavičius, Valdas; Razumienė, Julija; Ramanavičius, Arūnas; Ryabov, Alexander D.

doi:10.1016/j.bios.2004.05.012

Cited by 88 publications

(46 citation statements)

References 24 publications

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“…Good performance of several PQQ-dependent dehydrogenases with various ferrocene derivatives has been reported (Razumiene et al 2000(Razumiene et al , 2001Laurinavicius et al 2004). Extensive efforts were made to combine various mediators and immobilization techniques (Malinauskas et al 2004), and concluded that direct electron transfer from PQQ-glucose dehydrogenase did not occur without the presence of a mediator.…”

Section: Glucose and Ethanol In Beveragesmentioning

confidence: 99%

Gluconobacter in biosensors: applications of whole cells and enzymes isolated from gluconobacter and acetobacter to biosensor construction

et al. 2006

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Bacteria belonging to the genus Acetobacter and Gluconobacter, and enzymes isolated from them, have been extensively used for biosensor construction in the last decade. Bacteria used as a biocatalyst are easy to prepare and use in amperometric biosensors. They contain multiple enzyme activities otherwise not available commercially. The range of compounds analyzable by Gluconobacter biosensors includes: mono- and poly-alcohols, multiple aldoses and ketoses, several disaccharides, triacylglycerols, and complex parameters like utilizable saccharides or biological O2 demand. Here, the recent trends in Gluconobacter biosensors and current practical applications are summarized.

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Section: Glucose and Ethanol In Beveragesmentioning

confidence: 99%

Gluconobacter in biosensors: applications of whole cells and enzymes isolated from gluconobacter and acetobacter to biosensor construction

et al. 2006

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“…These metal ion conditions were chosen based on our recent report of DNA aptamers for PQQ, 10 but also represent conditions that could be used with other biomolecules. Calcium is well-known to enhance the enzyme activity of several PQQ-containing dehydrogenases 11,19 and has been investigated with immobilized PQQ on gold and platinum electrodes. 20 Ca 2+ and Mg 2+ were omitted from the phosphate buffer because of uncertain free concentrations due to potential chelation.…”

Section: Resultsmentioning

confidence: 99%

Electrochemistry of Pyrroloquinoline Quinone (PQQ) on Multi-Walled Carbon Nanotube-Modified Glassy Carbon Electrodes in Biological Buffers

Emahi

Mitchell

Baum

2017

J. Electrochem. Soc.

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Pyrroloquinoline quinone (PQQ) is an important electrocatalyst and redox cofactor for many enzymes used in bioanalytical applications. Careful selection of electrode modifications and buffer compositions is required due to PQQ's tendency to be irreversibly reduced on bare electrodes and the strong effect of pH on its electrochemistry. Multi-walled carbon nanotubes (MWCNTs) can effectively modify glassy carbon electrodes, but PQQ's behavior at these surfaces has not been investigated. While phosphate buffers have been used extensively to characterize PQQ's electrochemistry, phosphate buffers can act as chelating agents for important metal ions needed by PQQ and biomolecules. Different physiological buffers could overcome these challenges and expand the buffer options for biofuel cells. Using cyclic voltammetry, we studied PQQ in HEPES, MOPS, TRIS, and phosphate buffers at pH 7 with MWCNT-modified electrodes. While there were no significant differences in PQQ's behavior with the various buffers, the ionic composition of the non-phosphate buffers was very important. We observed that K + and Mg 2+ were the most influential ions for the reversible reduction-oxidation of PQQ. PQQ's electrochemistry was also affected by the length of and the functional groups on the MWCNTs, indicating that the electron transfer kinetics were quite sensitive to the surface chemistry of the electrodes. Pyrroloquinoline quinone (PQQ) is a redox cofactor found in the active sites of a number of enzymes. Most of these quinoproteins are dehydrogenases capable of catalyzing the oxidation of a broad range of substrates, such as alcohols, acids, aldehydes and sugars. 1 PQQ-dependent glucose dehydrogenase, for example, is capable of oxidizing several mono-and disaccharides, including glucose, maltose, lactose, galactose, xylose and mannose.1 The uniqueness of the quinoproteins and their broad substrate specificities have led to their use in bioanalytical applications, including biosensors and biofuel cells.In the absence of an enzyme, PQQ itself is capable of catalyzing redox reactions, including the oxidation of thiols, 2,3 amino acids, 4 and the redox cofactor nicotinamide adenine dinucleotide (NAD + ). 5The electrochemistry of PQQ has been studied quite extensively in both solution and immobilized forms via cyclic voltammetry (CV) and potentiometric titration studies. 6,7 These studies can be challenging due to the pH dependence of the redox potentials. 6,8,9 Using a cystamine-monolayer Au-modified electrode, Katz et al.6 studied the electrochemistry of PQQ in solution using CV and reported the change in potential with pH to be about 60 mV pH −1 at pH < 6.2; 30 mV pH −1 at 6.2 < pH < 8.6; and about 60 mV pH −1 at pH > 8.6. In another study using PQQ immobilized on few-walled carbon nanotube (FWCNT)-modified glassy carbon electrodes, the change in PQQ's redox potential was estimated to be approximately 67 mV pH −1 , with the highest reversible reduction-oxidation observed at ∼pH 2. The nature of the modified electrode surface can also affect P...

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“…Therefore, reports of GDH-based amperometric biosensors have been increasing. [6][7][8][9][10][11][12][13][14][15][16] A plasma-polymerized film (PPF) is synthesized by a glow discharge or plasma in the vapor phase. 17 The properties of the PPF include (a) the ability to be made extremely thin (<1 μm), (b) good adhesion to any substrate, (c) pinhole-free and flat surface structure, (d) mechanical and chemical stability (a result of its highly-branched and cross-linked structure), and (e) the capability to accommodate a large amount of biomolecules, such as enzymes, antibodies, and oligonucleotides, which can be immobilized onto the surface of the film.…”

Section: Introductionmentioning

confidence: 99%

“…Two kinds of GDH have been reported: one is NAD(P)-dependent [6][7][8][9][10][11][12] and the other is pyrroloquinoline quinone (PQQ)-dependent. [13][14][15][16] In this research, we chose NAD-dependent as a model of GDH family and here we report the usefulness and effectiveness of PPFs in GDH amperometric biosensors.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

2008

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Wiring of PQQ–dehydrogenases

Cited by 88 publications

References 24 publications

Gluconobacter in biosensors: applications of whole cells and enzymes isolated from gluconobacter and acetobacter to biosensor construction

Gluconobacter in biosensors: applications of whole cells and enzymes isolated from gluconobacter and acetobacter to biosensor construction

Electrochemistry of Pyrroloquinoline Quinone (PQQ) on Multi-Walled Carbon Nanotube-Modified Glassy Carbon Electrodes in Biological Buffers

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

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