Wirkungsmechanismus von Terrazol bei Mucor mucedo

Abstract

It is assumed that the fungistatic effect of terrazol in Mucor mucedo is induced by a liberation of phospholipases within the mitochondria and perhaps at other membranes. In isolated mitochondria a rapid formation of lysolecithin can be demonstrated at low concentrations of terrezol. This would explain the lytic symptoms in mitochondria visible by electron microscopy. Lipid peroxidation could not be demonstrated. The only antidots a t present known for the growth inhibiting effect of terrazol and on its ultrastructural effects are impure saccharose, which seems to contain an unknown factor, and procain hydrochloride or to a lesser extent lidocain, which are wellknown inhibitors of phospholipases. The pathologigal thickening of the cell wall induced by terrazol seems to be an unspecific side effect reflecting a diminished phosphorylating activity of the mitochondria. Uber einige Effekte von Terrazol, einem systeniischen Fungicid, auf Wachstuni, physiologische Aktivitaten so wie auf die Ultrastruktur von Mucor mucedo wurde von LYR et al. (1975) berichtet. Die geniessenen physiologischen Effekte beziiglich Pernieabilitat, At mung, Garung, Nucleinsauresynthese und Lipidsynthesen zeigten jedoch keine so eindeutige Blockierung, da13 man bereits auf eine Priniarwirkung schlienen kannte. Durch Zusatze von Lipiden, Vitaminen, Aniinosauren oder Nucleinsauren lie13 sich die durch Terrazol verursachte Wachstuniheniinung nicht aufheben. Mit Hilfe der Elektronenmikroskopie (CASFERSON u. LYR 1975) konnten aber typische Strukturveranderungen durch Terrazol sichtbar gemacht werden, die vor allem in einer Lysis der inneren Mitochondrieninemhran und einer anschliefienden Vakuolisierung der Mitochondrien bestehen. Daruber hinaus zeigt das Plasnialemma ein Abliisen von der Zellwand und Bildung von pinocytoseartigen Invaginationen. Die Zellwand wird in Ab-

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“…In Mucor mucedo (Zygomycete), a thickening of the cell wall was also observed induced by the fungicides pentachloronitrobenzene (PCNB) and terrazole, although in the latter case it appears to be a non-specific effect reflecting damage to mitochondria ( Casperson and Lyr, 1975 ; Lyr et al, 1977 ). However, in studies carried out with Phytophthora cactorum , PCNB did not cause any deformation of the wall ( Casperson and Lyr, 1975 ).…”

“…The observed effects are induced by a release of phospholipases within mitochondria, and perhaps other membranes. The decrease in phosphorylation activity in mitochondria had as a side effect a pathological thickening of the cell wall ( Lyr et al, 1977 ).…”