Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

Sarbishegi, Maryam; Khajavi, Ozra; Arab, Mohamed G.

doi:10.5812/numonthly.39284

Cited by 10 publications

(9 citation statements)

References 45 publications

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“…[14,15] Cyclic adenosine monophosphate is more important than cGMP for detrusor function, hence the cAMP pathway in the bladder has been more thoroughly investigated than cGMP. Five isoforms of PDEs (1)(2)(3)(4)(5) were isolated and demonstrated in human and porcine detrusor in functional studies. [16,17] The effect of vardenafil on cyclic nucleotide levels and bladder contraction was investigated in rat detrusor by Werkstrom et al [18] Relexation was accompanied with increased levels of both cAMP and cGMP, and it was proposed that vardenafil-induced relaxation of rat detrusor was mediated by cAMP through inhibition of cGMP dependent cAMP-PDE.…”

Section: Discussionmentioning

confidence: 99%

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Effect of sildenafil citrate in testosterone induced benign prostate hyperplasia rat model

Çalışkan

Keleş

Öztürk

et al. 2017

Turkish Journal of Urology

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Objective: Efficacy of treatments for benign prostate hyperplasia (BPH) is limited because the disease has complex etiopathogenesis. Recent studies have demonstrated the presence of phosphodiesterase-5 (PDE-5) receptors in prostate tissue. We investigated efficacy of sildenafil citrate in testosteron -induced BPH in rats. Material and methods:The rats were divided into three groups. Each groups had 7 rats. Group 1 was control group. Testosteron propionate 3 mg/kg/day was injected subcutaneously for two weeks in Group 2. The same procedure was done for Group 3 and sildenafil citrate was added to water at daily doses of 2 mg/kg for two weeks. The rats were euthanized with intraperitoneal pentobarbital. The body weights were measured and the prostates were removed. Results:The mean weights of rats were 288±31.93, 345±23.23 and 294±32.86 g in Groups 1, 2 and 3, respectively. The mean prostate weights of rats were 0.74±0.18, 1.3±0.13 and 0.72±0.24 g in Groups 1, 2, and 3, respectively. Group 2 had statistically significantly higher prostate weights than the other groups (p<0.01). Relative prostate weight is calculated with ratio of prostate weight to body weight. BPH group showed an increase in relative prostate weight compared with other groups with significant difference (p= 0.036 and p= 0.040). There was statistical difference for acinar area between Group 2 and the others, no significant difference of number of acini, interstitial space and epithelial thickness. Group 2 has more papillary projections per acini than the other groups. Conclusion:Favourable effect of sildenafil citrate on dimensions of prostate but not all on histological parameters was observed. We expect that PDE-5 inhibitors might be a treatment option for BPH patients if the studies support our findings in the future.

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Section: Discussionmentioning

confidence: 99%

“…The other factor that influences the BPH progression is inflammation of the prostate. [4] Most of the patients (79%) with BPH have also chronic prostatic inflammation. Inflammation associated-cytokines stimulate the cyclooxygenase-2 enzyme that increases proliferative rate and inhibits the cell death.…”

Section: Introductionmentioning

confidence: 99%

Effect of sildenafil citrate in testosterone induced benign prostate hyperplasia rat model

Çalışkan

Keleş

Öztürk

et al. 2017

Turkish Journal of Urology

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“…Fourteen days after BPH induction, prostate tissue sections were stained with toluidine blue as described previously [29,30] to identify mast cells. Every section was observed at a magnification of 100X.…”

Section: Staining Of Mast Cellsmentioning

confidence: 99%

Palmitoylethanolamide/Baicalein Regulates the Androgen Receptor Signaling and NF-κB/Nrf2 Pathways in Benign Prostatic Hyperplasia

et al. 2021

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Benign prostatic hyperplasia (BPH) is the most common benign tumor in males. Androgen/androgen receptor (AR) signaling plays a key role in the development of BPH; its alterations cause an imbalance between prostate cell growth and apoptosis. Furthermore, chronic inflammation and oxidative stress, which are common conditions in BPH, contribute to disrupting the homeostasis between cell proliferation and cell death. With this background in mind, we investigated the effect of ultramicronized palmitoylethanolamide (um-PEA), baicalein (Baic) and co-ultramicronized um-PEA/Baic in a fixed ratio of 10:1 in an experimental model of BPH. BPH was induced in rats by daily administration of testosterone propionate (3 mg/kg) for 14 days. Baic (1 mg/kg), um-PEA (9 mg/kg) and um-PEA/Baic (10 mg/kg) were administered orally every day for 14 days. This protocol led to alterations in prostate morphology and increased levels of dihydrotestosterone (DHT) and of androgen receptor and 5α-reductase expression. Moreover, testosterone injections induced a significant increase in markers of inflammation, apoptosis and oxidative stress. Our results show that um-PEA/Baic is capable of decreasing prostate weight and DHT production in BPH-induced rats, as well as being able to modulate apoptotic and inflammatory pathways and oxidative stress. These effects were most likely related to the synergy between the anti-inflammatory properties of um-PEA and the antioxidant effects of Baic. These results support the view that um-PEA/Baic should be further studied as a potent candidate for the management of BPH.

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“…The cyclooxygenase-2 (COX-2) enzyme converts arachidonic acid into prostaglandins (PGs), which plays a main role in inflammatory responses [ 6 ]. The coexistence of hyperplastic nodules and chronic inflammation in BPH tissue indicates the relationship between BPH and inflammation [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…The therapeutic methods of BPH include drug therapy (alpha-1 blockers, 5-alpha-reductase inhibitors) and operations (prostatectomy, transurethral resection, etc.) [ 6 ]. Although many patients take medicine for many years, the progress of BPH could not be halted and eventually develops into complications or the stage requiring surgical intervention [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Anti‐Inflammatory and Antioxidant Effects of Kelong‐Capsule on Testosterone‐Induced Benign Prostatic Hyperplasia in Rats

Zhang

Fan

Xie

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Benign prostatic hyperplasia (BPH) is a common disease in the current ageing male population. This research aims to study the effects of Kelong-Capsules (KLC) on testosterone-induced BPH. Thirty rats were randomly divided into normal group, model group, and three treatment groups. Three treatment groups were given KLC (3.6 g/kg), KLC (7.2 g/kg), and finasteride (0.9 mg/kg), respectively, for 28 days after establishing the animal model. The BPH rat models were evaluated by Traditional Chinese Medicine (TCM) symptoms and prostate index (PI). Results indicated that three treatment groups all alleviated the pathological changes of prostate and kidney at different levels. Compared with the model group, the PI of the groups treated with KLC (7.2 g/kg) and finasteride decreased significantly. The expressions of NF-E2 related factor 2 (Nrf-2) and quinine oxidoreductase (NQO1) in the group treated with KLC (3.6 g/kg) increased markedly (p < 0.01). The cyclooxygenase-2 (COX-2) protein expression of the group treated with KLC (7.2 g/kg) was increased (p < 0.01). In conclusion, KLC could obviously inhibit the growth of prostate, and KLC (3.6 g/kg) could promote the expressions of Nrf2 and NQO1.

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Withania coagulans Extract Induces Cell Apoptosis and Inhibits COX-2 Expression in a Rat Model of Benign Prostatic Hyperplasia

Cited by 10 publications

References 45 publications

Effect of sildenafil citrate in testosterone induced benign prostate hyperplasia rat model

Effect of sildenafil citrate in testosterone induced benign prostate hyperplasia rat model

Palmitoylethanolamide/Baicalein Regulates the Androgen Receptor Signaling and NF-κB/Nrf2 Pathways in Benign Prostatic Hyperplasia

Anti‐Inflammatory and Antioxidant Effects of Kelong‐Capsule on Testosterone‐Induced Benign Prostatic Hyperplasia in Rats

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