Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study

Alajbeg, Iva; Lapić, Ivana; Rogić, Dunja; Vuletić, Lea; Rogulj, Ana Andabak; Illeš, Davor; Zlatarić, Dubravka Knezović; Badel, Tomislav; Vrbanović, Ema; Alajbeg, Ivan

doi:10.1155/2017/2697464

Cited by 27 publications

(16 citation statements)

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“…Also, when validating the method in our previous study, some of the markers (i.e. afternoon MDA) showed better repeatability at a certain time of a day 27 .…”

Section: Enrollmentmentioning

confidence: 66%

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Effect of occlusal splint on oxidative stress markers and psychological aspects of chronic temporomandibular pain: a randomized controlled trial

Alajbeg

Vrbanović

Lapić

et al. 2020

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have their natural teeth and maintain good oral hygiene. Participants were not suitable if they were experiencing (1) inflammatory joint disease, (2) orofacial pain unrelated to TMD, (3) chronic systemic diseases (i.e. diabetes, cardiovascular diseases, malignancies, and autoimmune diseases), (4) causes of headache, unrelated to TMD, listed in the International Classification of Headache Disorders (ICDH II), (5) periodontitis, oral lesions and gum swelling. Individuals who had already been under treatment for TMD were excluded. Finally, participants who use supplements and medication known to affect the oxidative status, and smoke were not able to take part in the study. Study design. Allocation. To reduce the waiting time and potential withdrawal until a sufficient number of participants are involved, we have chosen a randomization procedure based on our experience of enrolling 6-8 participants in 2 months. The patients were assigned into two treatment groups utilizing random block randomization with a block size of 8 and 6. Randomization was computer-generated, and participants were randomly assigned to two treatment arms, SS or PS. The allocation was prepared by the study's statistician prior to enrollment of the participants. Blinding. Participants were unaware of the differences of each intervention. The principal investigator (IZA), who conducted the baseline evaluations, was the only person aware of the participants' treatment conditions. All follow-up assessments were performed by the investigator (EV), blinded to the group allocation. Observer training. Ten randomly selected subjects, different from the ones included in the investigation, underwent repeated clinical examinations by two experienced examiners to assess signs and symptoms of TMD (according to DC/TMD). No significant differences were recognized between the first and the second measurements (p = 0.87-0.89, paired t-test). The weighted kappa statistics showed adequate agreement between the observers (κ = 0.86-0.88).

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“…Also, when validating the method in our previous study, some of the markers (i.e. afternoon MDA) showed better repeatability at a certain time of a day 27 .…”

Section: Enrollmentmentioning

confidence: 66%

“…The sample collection and storage principles used in the study are described in detail by Alajbeg et al 27 .…”

Section: Enrollmentmentioning

confidence: 99%

Effect of occlusal splint on oxidative stress markers and psychological aspects of chronic temporomandibular pain: a randomized controlled trial

Alajbeg

Vrbanović

Lapić

et al. 2020

Sci Rep

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“…Urinary biomarkers of oxidative stress were measured in samples from 4 study visits across pregnancy indicating variable unadjusted 8-oxodG concentrations with ICC = 0.32 38 . Saliva of healthy adults was collected over 3 consecutive days to assess unadjusted 8-oxodG diurnal variations and intra-individual CVs of 40% samples was higher than 35%, which was calculated by the following equation, (between-day SD/between-day mean) × 100, indicating high intra-individual variability 39 . SG and creatinine were proved to be temporal consistent in urina sanguinis collected from 243 subjects over 5 consecutive weekdays 40 .…”

Section: Discussionmentioning

confidence: 99%

Classification and Temporal Variability in Urinary 8-oxodG and 8-oxoGuo: Analysis by UHPLC-MS/MS

Yao

Zhang

et al. 2019

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Oxidative stress damage has been found to be associated with exposure of children to environmental pollutants, but there are few data on the variability of urinary oxidative stress biomarkers and the accuracy of biomarker concentration classification. We performed a longitudinal study in Chinese school-aged children to investigate the variability of urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) concentrations and the ability of a single first morning urine sample to assess accuracy and sensitivity of biomarkers concentration classification. After adjusting for both creatinine and specific gravity, we characterized the distribution and reproducibility of repeated measurement of 8-oxodG and 8-oxoGuo by using intraclass correlation coefficients (ICCs) derived from linear mixed model and performed surrogate category analyses to determine whether a single spot sample could accurately classify 8-oxodG and 8-oxoGuo levels. Results indicated that the geometric mean (GM) concentrations of 8-oxodG and 8-oxoGuo were 3.865 ng/mL and 5.725 ng/mL, respectively. High variability of 8-oxodG and 8-oxoGuo was observed in the single spot first morning urine sample (ICC = 0.25 and 0.18, respectively). Three repeated urinary specimens achieved sensitivity of 0.87 for 8-oxodG and 0.83 for 8-oxoGuo in low tertile and sensitivity of 0.78 in high tertile. But classification in medium tertile was less accurate for both 8-oxodG and 8-oxoGuo. In conclusion, high variability of urinary 8-oxodG and 8-oxoGuo levels results in repeated samplings needed for accurate classification.

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“…The methods of sample collection and analysis were described in detail and validated by our group in a prior study ( Alajbeg et al, 2017 ). The subjects were instructed to fast before saliva collection in the morning and to not eat or drink anything but water at least 2 h before sampling in the afternoon.…”

Section: Methodsmentioning

confidence: 99%

“…For these assays, analytical performance, including intra- and inter-assay variability, was assessed. The data and a detailed description of the methodology is available in our previous study ( Alajbeg et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Salivary Oxidant/Antioxidant Status in Chronic Temporomandibular Disorders Is Dependent on Source and Intensity of Pain – A Pilot Study

et al. 2018

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Temporomandibular disorders (TMD) have been associated with altered salivary oxidative status, but the relation with pain source and pain severity isn’t clarified. With the aim to assess their interaction with TMD, we compared levels of selected salivary oxidative stress (OS) markers (glutathione peroxidase, superoxide dismutase, total antioxidant capacity (TAC), uric acid, 8-hydroxydeoxyguanosine, malondialdehyde) and salivary cortisol (SC) as a stress indicator, between 20 TMD patients and 15 healthy control subjects. In order to record differences relating to pain source and severity, patients were respectively classified according to specific diagnoses (myofascial pain or disc displacement (DD)), and pain intensity (high or low). TAC was significantly higher in TMD patients than in controls (morning p = 0.015; afternoon p = 0.005). Significant differences were also observed when TAC levels between high-intensity pain patients and controls were compared, as well as between DD patients and controls. In logistic regression analysis, higher levels of TAC were related to DD (morning OR: 1.66, 95%CI: 1.05–2.64, p = 0.029; afternoon OR: 2.10, 95%CI: 1.11–3.98, p = 0.021) and to high-intensity pain (morning OR: 1.81, 95%CI: 1.04–3.15, p = 0.037; afternoon OR: 1.79, 95%CI: 1.02–3.14, p = 0.043). We also found that morning SC was positively correlated with antioxidant parameters in TMD patients. Our data suggest compensatory mechanism as response to higher level of stress. This stress could be extrinsic and lead toward TMD, or intrinsic, emerging from established TMD, or could be both. The intensity and the source of pain should be considered important factors in future investigations evaluating salivary OS markers in TMD patients.

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Within-Subject Reliability and between-Subject Variability of Oxidative Stress Markers in Saliva of Healthy Subjects: A Longitudinal Pilot Study

Cited by 27 publications

References 50 publications

Effect of occlusal splint on oxidative stress markers and psychological aspects of chronic temporomandibular pain: a randomized controlled trial

Effect of occlusal splint on oxidative stress markers and psychological aspects of chronic temporomandibular pain: a randomized controlled trial

Classification and Temporal Variability in Urinary 8-oxodG and 8-oxoGuo: Analysis by UHPLC-MS/MS

Salivary Oxidant/Antioxidant Status in Chronic Temporomandibular Disorders Is Dependent on Source and Intensity of Pain – A Pilot Study

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