Wittgenstein's Heirs and Editors

Erbacher, Christian

doi:10.1017/9781108878111

Cited by 18 publications

(2 citation statements)

References 30 publications

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“…55 Accordingly, the use of CQ in the evaluation of delivery systems has become increasingly prevalent as CQ largely increases delivery system efficiency via the proton sponge effect. [56][57][58][59] Upon protonation, CQ neutralizes the acidification of endosomes, preventing endosomal-lysosomal fusion, and inducing osmotic swelling, leading to vesicle rupture. 60,61 In these studies, CQ is a useful chemical tool, because when combined with data analyzing total delivered protein content, it can allow calculation of the ratio of uptake to endosomal escape.…”

Section: Resultsmentioning

confidence: 99%

Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP

et al. 2021

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The ability for biologics to access intracellular targets hinges on the translocation of active, unmodified protein. This is often achieved using nanoscale formulations, which enter cells through endocytosis. This uptake mechanism often limits the therapeutic potential of the biologics, as the propensity of the nanocarrier to escape the endosome becomes the key determinant. To appropriately evaluate and compare competing delivery systems of disparate compositions, it is therefore critical to assess endosomal escape efficiencies. Unfortunately, quantitative tools to assess endosomal escape are lacking and standard approaches often lead to erroneous interpretation of cytosolic localization. In this study, we use a split-complementation endosomal escape (SEE) assay to evaluate levels of cytosolic caspase-3 following delivery by polymer nanogels and mesoporous silica nanoparticles. In particular, we use SEE as a means to enable systematic investigation of the effect of polymer composition, polymer architecture (random vs. block), hydrophobicity, and surface functionality. Although polymer structure had little influence on endosomal escape, nanogel functionalization with cationic and pH-sensitive peptides significantly enhanced endosomal escape levels and further, significantly increased the amount of nanogel per endosome. This work serves as a guide for developing an optimal caspase-3 delivery system, as this caspase-3 variant can be easily substituted for a therapeutic caspase-3 cargo in any system that results in cytosolic accumulation and cargo release. In addition, these data provide a framework that can be readily applied to a wide variety of protein cargos to assess the independent contributions of both uptake and endosomal escape of a wide range of protein delivery vehicles.

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Section: Resultsmentioning

confidence: 99%

Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP

et al. 2021

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“…One of the most important effects of IDP is that it creates in the user an awareness that what she is dealing with is not something that simply falls from the sky: that even premade editions do not fall from the sky, but result from selection and decision processes. Where the user previously, maybe with a sort of innocent and uncritical attitude, simply received and accepted what the editors -be it Wittgenstein's heirs (Erbacher, 2020) or othersgave her (Pichler a.o., 2011), with IDP she suddenly recognizes that any edition is a product of human action deserving scrutiny and verification. The user may just as well take an active role herself in the making of the edition, and through IDP she can indeed become a co-agent, taking on some of the editorial responsibilities herself.…”

Section: Diplomatic Transcription Of "Typescript Only"mentioning

confidence: 99%

Complementing Static Scholarly Editions with Dynamic Research Platforms

Pichler¹

2021

Linköping Electronic Conference Proceedings

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In 2000 the Wittgenstein Archives at the University of Bergen (WAB) published the CD-ROM edition of Wittgenstein's Nachlass: The Bergen Electronic Edition (BEE). However, since the publication, it has become increasingly obvious that this edition does not meet all demands of the community that uses Wittgenstein's manuscripts (his "Nachlass") for research and learning. WAB has, therefore, for more than a decade now worked towards complementing the static CD-ROM edition with an interactive web platform that allows a more comprehensive, yet also a more tailored and user-specific utilization, of WAB's Nachlass resources. The paper describes and discusses two specific web service tools of this platform: Interactive Dynamic Presentation (IDP) of the Wittgenstein Nachlass, and Semantic Faceted Search and Browsing (SFB) of Wittgenstein metadata. The paper argues that it is only when these two tools are fully implemented and functional that WAB can adequately serve the scholarly needs of the Wittgenstein Nachlass user community. The paper describes some selected features and functionalities of these two tools in detail. While pilot versions of both tools are already in use on the platform, they need substantial extension and optimization. This upgrade is being implemented within the Norwegian CLARINO+ project. Data and Metadata for Use of Wittgenstein in Research and EducationDuring his lifetime, the Austrian-British philosopher Ludwig Wittgenstein (1889-1951) published only one philosophical book, the Logisch-philosophische Abhandlung / Tractatus logicophilosophicus (1 st ed. 1921/22), and a Dictionary for Elementary Schools (1 st ed. 1926). However, on his death in 1951, he left behind a significant 20,000 page corpus of unpublished philosophical notebooks, manuscripts, typescripts and dictations. This oeuvre, called "the Wittgenstein papers" or "Wittgenstein's Nachlass" (von Wright, 1969), was early recognized to be one of the most important philosophy archives of all times. It was subsequently brought to the wider public through posthumous book publications such as Philosophical Investigations (1 st ed. 1953) and Culture and Value (1 st ed. 1977).The practice of bringing the Nachlass to digital users reached its first milestone in 1998 with Vol. 1 of the Bergen CD-ROM edition Wittgenstein's Nachlass: The Bergen Electronic Edition (BEE;Wittgenstein, 2000), edited by the Wittgenstein Archives at the University of Bergen (WAB, http://wab.uib.no/). 1 The edition became notable for creating unprecedented new access and research possibilities (Meschini, 2020, ch. 4). Since its establishment in 1990, WAB has worked towards providing digital data and metadata for using the Wittgenstein Nachlass in research and education (Huitfeldt, 2006). This includes the creation of machine-readable transcriptions with specialized markup. The transcriptions were originally produced in MECS-WIT (Huitfeldt, 1994). But at present they are maintained in XML TEI format (Pichler, 2010). XML TEI transcription samples of 5000 Nachlass pages were ma...

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In Search of the Redeeming Word: Wittgenstein’s Private Notebooks 1914–16 and the Making of the Tractatus

Perloff

2023

Vienna Circle Institute Yearbook

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Wittgenstein's Heirs and Editors

Abstract: Bibliographie der deutsch-und englischsprachigenWittgenstein-Ausgaben 1 GliederungÜbersicht zu den Ausgaben mit Quellen im Nachlass ..

Cited by 18 publications

References 30 publications

Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP

Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP

Complementing Static Scholarly Editions with Dynamic Research Platforms

In Search of the Redeeming Word: Wittgenstein’s Private Notebooks 1914–16 and the Making of the Tractatus

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