Wnt11 Signaling Promotes Proliferation, Transformation, and Migration of IEC6 Intestinal Epithelial Cells

Ouko, Lillian; Ziegler, Thomas R.; Gu, Li H.; Eisenberg, Leonard M.; Yang, Vincent W.

doi:10.1074/jbc.m402877200

Cited by 93 publications

(75 citation statements)

References 88 publications

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“…A common theme is that Wnt11 acts locally to control the turnover of proteins involved in cell-cell and cell-substrate adhesion and thereby facilitate coordinated cell migration (Katoh, 2009;Uysal and Kypta, 2011). Wnt11 increased migration of LNCaP and PC3 prostate cancer cells (Dwyer et al, 2010;Uysal-Onganer et al, 2010), MDA MB 231 breast cancer cells, HCT116 colon cancer cells (Dwyer et al, 2010) and non-transformed rat small intestinal epithelail cells (Ouko et al, 2004). Correpondingly, the present study showed Wnt11-induced increased cell migration of SKOV-3 cells.…”

Section: Discussionsupporting

confidence: 59%

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Jannesari-Ladani

Hossein²,

Izadi-Mood³

2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 59%

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Jannesari-Ladani

Hossein²,

Izadi-Mood³

2014

Asian Pacific Journal of Cancer Prevention

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“…Within the Xbp1 +/+(IEC) crypts may have a role as a trans-acting factor and deserves further exploration in vivo. Wnt11 has been shown to be expressed at elevated levels in patients with ulcerative colitis (You et al, 2008), gastric carcinoma cell lines, and primary CRC cells (Uysal-Onganer and Kypta, 2012), and it can induce the proliferation and transformation of the IEC6 line in vitro (Ouko et al, 2004). Analogous to the model proposed in this paper, Paneth cells have recently been reported to sense calorie restriction (via mTORC1) and orchestrate ISC expansion in trans (Yilmaz et al, 2012).…”

Section: Isc Expansion Is Individually Determined In Each Xbp1-deficimentioning

confidence: 79%

ER stress transcription factor Xbp1 suppresses intestinal tumorigenesis and directs intestinal stem cells

Niederreiter¹,

Fritz²,

Adolph³

et al. 2013

J Cell Biol

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“…The possibility that neural crest was induced in the graft is ruled out, as competence for neural crest induction is lost at the stage at which the tissue was transplanted (Mancilla and Mayor, 1996;Bastidas et al, 2004). It is still possible that Wnt11 promotes cell proliferation (Ouko et al, 2004), although this seems unlikely as cell numbers in our in vitro cultures did not increase after stimulating Wnt11 signalling. We, therefore, favour an effect of Wnt11 on neural crest migration, instead of cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Essential role of non-canonical Wnt signalling in neural crest migration

et al. 2005

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Migration of neural crest cells is an elaborate process that requires the delamination of cells from an epithelium and cell movement into an extracellular matrix. In this work, it is shown for the first time that the non-canonical Wnt signalling [planar cell polarity (PCP) or Wnt-Ca2+] pathway controls migration of neural crest cells. By using specific Dsh mutants, we show that the canonical Wnt signalling pathway is needed for neural crest induction, while the non-canonical Wnt pathway is required for neural crest migration. Grafts of neural crest tissue expressing non-canonical Dsh mutants, as well as neural crest cultured in vitro, indicate that the PCP pathway works in a cell-autonomous manner to control neural crest migration. Expression analysis of non-canonical Wnt ligands and their putative receptors show that Wnt11 is expressed in tissue adjacent to neural crest cells expressing the Wnt receptor Frizzled7 (Fz7). Furthermore, loss- and gain-of-function experiments reveal that Wnt11 plays an essential role in neural crest migration. Inhibition of neural crest migration by blocking Wnt11 activity can be rescued by intracellular activation of the non-canonical Wnt pathway. When Wnt11 is expressed opposite its normal site of expression, neural crest migration is blocked. Finally, time-lapse analysis of cell movement and cell protrusion in neural crest cultured in vitro shows that the PCP or Wnt-Ca2+ pathway directs the formation of lamellipodia and filopodia in the neural crest cells that are required for their delamination and/or migration.

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Wnt11 Signaling Promotes Proliferation, Transformation, and Migration of IEC6 Intestinal Epithelial Cells

Cited by 93 publications

References 88 publications

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

ER stress transcription factor Xbp1 suppresses intestinal tumorigenesis and directs intestinal stem cells

Essential role of non-canonical Wnt signalling in neural crest migration

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