Wnt3 gene expression promotes tumor progression in non-small cell lung cancer

Nakashima, Nariyasu; Liu, Dage; Huang, Cheng‐Long; Ueno, Masaki; Zhang, Xia; Yokomise, Hiroyasu

doi:10.1016/j.lungcan.2011.10.007

Cited by 50 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, we previously demonstrated that RA signaling activation leads to the spontaneous regression of Keratoacanthoma skin tumors by inhibiting Wnt pathway and stimulating terminal differentiation 8 . In addition, several evidences in the past clearly showed that Wnt signaling activation is required for NSCLC growth and metastatic progression 26, 27 . For all the findings described above, we reasoned that RA could affect Wnt activation, along with EGFR inhibition, thus justifying NSCLC tumor growth arrest.…”

Section: Resultsmentioning

confidence: 99%

Retinoic Acid affects Lung Adenocarcinoma growth by inducing differentiation via GATA6 activation and EGFR and Wnt inhibition

Zito

Naselli

Saieva

et al. 2017

Sci Rep

View full text Add to dashboard Cite

A fundamental task in cancer research aims at the identification of new pharmacological therapies that can affect tumor growth. Differentiation therapy might exploit this function not only for hematological diseases, such as acute promyelocytic leukemia (APML) but also for epithelial tumors, including lung cancer. Here we show that Retinoic Acid (RA) arrests in vitro and in vivo the growth of Tyrosine Kinase Inhibitors (TKI) resistant Non Small Cell Lung Cancer (NSCLC). In particular, we found that RA induces G0/G1 cell cycle arrest in TKI resistant NSCLC cells and activates terminal differentiation programs by modulating the expression of GATA6, a key transcription factor involved in the physiological differentiation of the distal lung. In addition, our results demonstrate that RA inhibits EGFR and Wnt signaling activation, two pathways involved in NSCLC progression. Furthermore, we uncovered a novel mechanism in NSCLC that shows how RA exerts its function; we found that RA-mediated GATA6 activation is necessary for EGFR and Wnt inhibition, thus leading to 1) increased differentiation and 2) loss of proliferation. All together, these findings prove that differentiation therapy might be feasible in TKI resistant NSCLCs, and shed light on new targets to define new pharmacological therapies.

show abstract

Section: Resultsmentioning

confidence: 99%

Retinoic Acid affects Lung Adenocarcinoma growth by inducing differentiation via GATA6 activation and EGFR and Wnt inhibition

Zito

Naselli

Saieva

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Wingless-type MMTV integration site family, member 3 (Wnt3), a secreted glycoprotein, located on human chromosome 17 and an upstream protein of the canonical Wnt/β-catenin pathway (31), was predicted as a potential target gene of miR-1247-5p and this was ascertained by luciferase reporter assay and western blotting. Previous studies demonstrated that the canonical Wnt/β-catenin pathway participates in the genesis and progression of several cancers by activating the transcription of its downstream genes (32)(33)(34).…”

Section: Discussionmentioning

confidence: 99%

miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In addition, survivin has been correlated with tumor aggression and a poor prognosis for many cancers including melanoma [37]. The importance of this effect is clearly indicated by the reduced expression of c-Myc, and survivin proteins play a critical role in tumor cell proliferation and survival [38]. …”

Section: Discussionmentioning

confidence: 99%

The Antitumor Activity ofAntrodia camphoratain Melanoma Cells: Modulation of Wnt/β-Catenin Signaling Pathways

Hseu

Tsou

Kumar

et al. 2012

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine. The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin signaling pathway. In this study, we observed that AC treatment resulted in decreased cell viability and disturbed Wnt/β-catenin cascade in B16F10 and/or B16F1 melanoma cells. This result was accompanied by a decrease in the expression of Wnt/β-catenin transcriptional targets, including c-Myc and survivin. Furthermore, treatment of melanoma cells with AC resulted in a significant increase in apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9 and -3 activation, PARP degradation, Bcl-2/Bax dysregulation, and p53 expression. We also observed that AC caused G1 phase arrest mediated by a downregulation of cyclin D1 and CDK4 and increased p21 and p27 expression. In addition, we demonstrated that non- and subcytotoxic concentrations of AC markedly inhibited migration and invasion of highly metastatic B16F10 cells. The antimetastatic effect of AC was further confirmed by reductions in the levels of MMP-2, MMP-9, and VEGF expression. These results suggest that Antrodia camphorata may exert antitumor activity by downregulating the Wnt/β-catenin pathways.

show abstract

Wnt3 gene expression promotes tumor progression in non-small cell lung cancer

Cited by 50 publications

References 33 publications

Retinoic Acid affects Lung Adenocarcinoma growth by inducing differentiation via GATA6 activation and EGFR and Wnt inhibition

Retinoic Acid affects Lung Adenocarcinoma growth by inducing differentiation via GATA6 activation and EGFR and Wnt inhibition

miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3

The Antitumor Activity ofAntrodia camphoratain Melanoma Cells: Modulation of Wnt/β-Catenin Signaling Pathways

Contact Info

Product

Resources

About