Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes

Dutra, Heverton Leandro Carneiro; Rocha, Marcele Neves; Dias, Fernando Braga Stehling; Mansur, Simone Brutman; Caragata, Eric P.; Moreira, Luciano Andrade

doi:10.1016/j.chom.2016.04.021

Cited by 471 publications

(427 citation statements)

References 28 publications

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“…22 . Examples include the bacterial endosymbiont Wolbachia, which enhances resistance to Drosophila C virus infection in flies 10,131 and to dengue or chikungunya virus infections in mosquitoes 132,168 . This protective effect has been linked to priming of the mosquito innate immune system and possibly competition for resources supporting pathogen replication.…”

Section: Box 3 Microbiota and Disease Tolerancementioning

confidence: 99%

Disease tolerance and immunity in host protection against infection

2017

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The immune system has most likely evolved to limit the negative impact exerted by pathogens on host homeostasis. This defense strategy relies on the concerted action of innate and adaptive components of the immune system, which sense and target pathogens for containment, destruction or expulsion. Resistance to infection refers to these immune functions, which reduce the pathogen load of an infected host as the means to preserve homeostasis. Immune-driven resistance to infection is coupled to an additional, and arguably as important, defense strategy that limits the extent of dysfunction imposed to host parenchyma tissues during infection, without exerting a direct negative impact on pathogens.This defense strategy, called disease tolerance, relies on tissue damage control mechanisms that prevent the deleterious effects of pathogens, while uncoupling immunedriven resistance mechanisms from immunopathology and disease. Here we provide a unifying view of resistance and disease tolerance within the framework of immunity to infection.

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Section: Box 3 Microbiota and Disease Tolerancementioning

confidence: 99%

Disease tolerance and immunity in host protection against infection

2017

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“…However, some of the major mosquito vectors are devoid of Wolbachia, but when transinfected with Wolbachia from another insect host, virus protection is conferred (Moreira et al, 2009;Xi et al, 2005). For example, in Aedes aegypti mosquitoes stably transinfected with Wobachia, replication of several medically important arboviruses, replication of several medically important arboviruses, such as dengue virus, Zika virus, Chikungunya virus and West Nile virus (WNV), is blocked (Bian et al, 2010;Dutra et al, 2016;Hussain et al, 2013;Moreira et al, 2009). Here, we investigated the effect of Wolbachia on an ISF, cell fusing agent virus (CFAV), in the A. aegypti Aag2 cell line persistently infected with the virus.…”

mentioning

confidence: 99%

Wolbachia suppresses cell fusing agent virus in mosquito cells

Zhang

Etebari

Asgari

2016

Journal of General Virology

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The genus Flavivirus contains a large number of positive-sense ssRNA viruses. While some are transmitted by mosquitoes or other arthropods and are pathogenic to humans and animals (e.g. dengue and Zika viruses), some are insect-specific and do not replicate in vertebrate cells. These are known as insect-specific flaviviruses (ISFs). Cell fusing agent virus (CFAV) was the first described ISF, which was detected in an Aedes aegypti cell line, Aag2. Here, we investigated the effect of Wolbachia, a widespread endosymbiont of many insect species, that is known to block replication of several pathogenic flaviviruses, on CFAV. Our results demonstrated that, in mosquito cells, Wolbachia vastly suppresses replication of CFAV, with significantly less CFAV viral interfering small RNAs produced in the cells. However, removal of Wolbachia with tetracycline led to increased CFAV replication. These results suggest that Wolbachia is also able to suppress an ISF.

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“…aegypti populations. This sharply contrasts previous studies where Wolbachia infection significantly reduced the proportion of individuals infected with other flaviviruses such as DENV (Amuzu & McGraw, 2016; Amuzu, Simmons, & McGraw, 2015; Bian et al., 2013; Frentiu et al., 2014; Moreira et al., 2009; Walker et al., 2011), Zika (Aliota et al., 2016; Dutra et al., 2016), and YFV (van den Hurk et al., 2012). Our findings are, however, supported by studies performed in Cx.…”

Section: Discussionmentioning

confidence: 99%

“…albopictus (Xi, Dean, Khoo, & Dobson, 2005), and w Mel w AlbB (Joubert et al., 2016) that is a superinfection of w Mel and w AlbB. Wolbachia ‐mediated pathogen blocking has now been observed for arboviruses such as WNV (Glaser & Meola, 2010), YFV (van den Hurk et al., 2012), DENV (Bian et al., 2010; Frentiu, Robinson, Young, McGraw, & O'Neill, 2010; Moreira et al., 2009; Walker et al., 2011), ZIKV (Aliota, Peinado, Velez, & Osorio, 2016; Dutra et al., 2016), and Chikungunya virus (van den Hurk et al., 2012; Moreira et al., 2009). Wolbachia is currently being released into populations of Ae.…”

Section: Introductionmentioning

confidence: 99%

Wolbachia enhances insect‐specific flavivirus infection in Aedes aegypti mosquitoes

Amuzu

Tsyganov

Koh

et al. 2018

Ecology and Evolution

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Mosquitoes transmit a diverse group of human flaviviruses including West Nile, dengue, yellow fever, and Zika viruses. Mosquitoes are also naturally infected with insect‐specific flaviviruses (ISFs), a subgroup of the family not capable of infecting vertebrates. Although ISFs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. Wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits the replication of co‐infecting pathogens, including flaviviruses. Artificially created Wolbachia‐infected Aedes aegypti mosquitoes are being released into the wild in a series of trials around the globe with the hope of interrupting dengue and Zika virus transmission from mosquitoes to humans. Our work investigated the effect of Wolbachia on ISF infection in wild‐caught Ae. aegypti mosquitoes from field release zones. All field mosquitoes were screened for the presence of ISFs using general degenerate flavivirus primers and their PCR amplicons sequenced. ISFs were found to be common and widely distributed in Ae. aegypti populations. Field mosquitoes consistently had higher ISF infection rates and viral loads compared to laboratory colony material indicating that environmental conditions may modulate ISF infection in Ae. aegypti. Surprisingly, higher ISF infection rates and loads were found in Wolbachia‐infected mosquitoes compared to the Wolbachia‐free mosquitoes. Our findings demonstrate that the symbiont is capable of manipulating the mosquito virome and that Wolbachia‐mediated viral inhibition is not universal for flaviviruses. This may have implications for the Wolbachia‐based DENV control strategy if ISFs confer fitness effects or alter mosquito susceptibility to other flaviviruses.

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Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes

Cited by 471 publications

References 28 publications

Disease tolerance and immunity in host protection against infection

Disease tolerance and immunity in host protection against infection

Wolbachia suppresses cell fusing agent virus in mosquito cells

Wolbachia enhances insect‐specific flavivirus infection in Aedes aegypti mosquitoes

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