Wolbachia Infection in Flea Populations (Insecta: Siphonaptera)

Yudina, M. A.; Bykov, R. A.; Kotti, B. K.; Высочина, Н. П.; Stakheev, V. V.; Broshkov, A. D.; Захаров, И. К.; Ilinsky, Yu. Yu.

doi:10.1134/s2079086419050098

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…The symbiont was found in all populations of C. t. altaicus, and Wolbachia infection was found in C. t. dzetysuensis (MF045776-MF045779 and MF04583-MF045786). Notably, Wolbachia infection is often associated with flea diversity as summarized by Yudina et al [38]. The sequence of the Wolbachia ftsZ gene isolated from C. t. altaicus in our isolates was clustered into the A-supergroup.…”

Section: Discussionsupporting

confidence: 52%

Genetic Evidence of an Isolation Barrier between Flea Subspecies of Citellophilus tesquorum (Wagner, 1898) (Siphonaptera: Ceratophyllidae)

Ilinsky

Lapshina

Verzhutsky

et al. 2022

Insects

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This study investigated the relationship between two subspecies of the Citellophilus tesquorum flea, C. t. altaicus and C. t. sungaris, which are vectors of the bacterium Yersinia pestis that causes human plague across the vast territories of the Palearctic. Adult fleas were collected from 16 localities and 11 populations in 2019 and 2020. Specimens were morphologically verified for subspecies status and analysed for mitochondrial cytochrome c oxidase subunit I (COI) DNA, nuclear ribosomal cluster internal transcribed spacer 1 (ITS1) and ITS2, and Wolbachia-infection status. Our results demonstrated a genetic difference between C. t. altaicus and C. t. sungaris. According to mitochondrial data, the genetic distance between clades of C. t. altaicus and C. t. sungaris was comparable with the species divergence of the genus Callopsylla, which is closely related to Citellophilus. All studied populations of C. t. altaicus were Wolbachia-infected, whereas all studied populations of C. t. sungaris were symbiont-free. Data for ITS1 and ITS2 had much lower phylogenetic signals than mitochondrial data; however, diagnostic substitutions for C. t. altaicus and C. t. sungaris delimitation were also revealed. Analysis of a hardly accessible report on cross experiments allowed us to conclude the partial postzygotic isolation between these subspecies. Taken together, the molecular-genetic, geographical, morphological, and reproductive isolation findings verified that C. t. altaicus and C. t. sungaris subspecies could be considered as different species.

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Section: Discussionsupporting

confidence: 52%

Genetic Evidence of an Isolation Barrier between Flea Subspecies of Citellophilus tesquorum (Wagner, 1898) (Siphonaptera: Ceratophyllidae)

Ilinsky

Lapshina

Verzhutsky

et al. 2022

Insects

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“…In Scolytine, cases of low Wolbachia titers were found in populations of Pityogenes chalcographus [29]; however, there was no comparison between males and females. Therefore, there are few examples of sex-specific differences in Wolbachia titers, whereas there are a number of low symbiont titers in other species [29,[48][49][50][51][52][53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Sex-Specific Wolbachia Infection Patterns in Populations of Polygraphus proximus Blandford (Coleoptera; Curculionidae: Scolytinae)

Bykov

Керчев

Demenkova

et al. 2020

Insects

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Wolbachia symbionts are maternally inherited bacteria that are widely distributed among Arthropoda hosts. Wolbachia influence their host biology in diverse ways. They may induce reproductive abnormalities, protect hosts against pathogens and parasites, or benefit hosts through metabolic provisioning. The progeny of an infected female are ordinarily infected with Wolbachia; however, Wolbachia have no future in male host progeny because they cannot transmit the symbiont to the next generation. Here, we analyze native and invasive populations of the four-eyed fir bark beetle (Polygraphus proximus) for Wolbachia prevalence and symbiont genetic diversity. This species is a dangerous pest of Siberian fir (Abies sibirica) forests. The native range of P. proximus includes the territories of the Russian Far East, Japan, Korea, and Northeast China, whereas its invasive range includes West Siberia, with further expansion westward. Surprisingly, we revealed a difference in the patterns of Wolbachia prevalence for males and females. Infection rate and Wolbachia titers were higher in females than in males. ST-533, the only haplotype of Wolbachia supergroup B, was associated with a minimum of three out of the five described mitochondrial haplotypes.

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“…Образец гомогенизируют в буфере, содержащем NaCl, ЭДТА для связывания двухвалентных катионов и ингибирования нуклеаз, Трис, поддерживающий буферные свойства, а также детергент, разрушающий мембраны и связывающийся с белками (SDS, CTAB, DEPS и др.). Конечные концентрации компонентов в экстрагирующем буфере могут заметно варьировать, что зависит от того, происходит ли высаливание во время экстракции или после (Liu et al, 2000;Rivero et al, 2006;Lagisz et al, 2010а;Ilinsky, 2013;Tian, Yu, 2013;Nguyen et al 2017;Yudina et al, 2019;Wang, Wang, 2012;Asghar et al, 2015;Быков и др., 2020). Гомогенат инкубируют обычно при температурах от 37 °C до 60 °C, при этом чем ниже температура, тем дольше инкубация, хотя и здесь бывают вариации (Lagisz et al, 2010а;Bykov et al, 2020).…”

Section: экстракция днкunclassified

Introduction to Molecular Diagnostics of Insects

et al. 2021

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Insects play an important role in biocenoses due to their abundance and wide (cosmopolitan) distribution. Many insects are crop pests. An effective pest control could be realized in case of proper species identification, which is usually managed by morphological analysis. Molecular methods allow to deep study of many issues of insect biology. In particular, traditional approach can not ordinary identify a species at all stages of their life cycle, whereas molecular methods can it. This review covers a wide range of issues related to the molecular genetic analysis of insects. In the first section we consider the methods of fixation and storage of insect specimens, as well as their impact on DNA quality. Further, we provide general information on population study design. Various schemes of DNA extraction, examples of both express techniques and more thorough protocols for DNA extraction and their purification are provided. In addition, methods of DNA isolation that allow to preserve a specimen integrity for further morphological studies are considered. The methods of DNA quality control are described in detail, that is important for PCR analysis. The last section provides various methods of PCR analysis, that we exemplify by studies aimed to elucidate both fundamental issues and practical problems.

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Wolbachia Infection in Flea Populations (Insecta: Siphonaptera)

Cited by 4 publications

References 41 publications

Genetic Evidence of an Isolation Barrier between Flea Subspecies of Citellophilus tesquorum (Wagner, 1898) (Siphonaptera: Ceratophyllidae)

Genetic Evidence of an Isolation Barrier between Flea Subspecies of Citellophilus tesquorum (Wagner, 1898) (Siphonaptera: Ceratophyllidae)

Sex-Specific Wolbachia Infection Patterns in Populations of Polygraphus proximus Blandford (Coleoptera; Curculionidae: Scolytinae)

Introduction to Molecular Diagnostics of Insects

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