Wolbachia infection in populations of the coniferous forest pest Dendrolimus superans sibiricus Tschetverikov, 1908 (Lepidoptera: Lasiocampidae)

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“…The amplicons were visualized using electrophoresis in 1 % agarose gels with GeneRuler Ladder Mix molecular weight marker, 75-20000 bp (Thermo Fisher Scientific). The 95 % confidence intervals were estimated using the Clopper-Pearson method (Clopper, Pearson, 1934) which is routinely used when Wolbachia prevalence rates in small samples are examined (Yudina et al, 2016, Bykov et al, 2019. Estimates of the data reliability were obtained using the exact Fisher's test (Fisher, 1922), Pearson's chi-square criterion and chi-square criterion with Yates correction (Yates, 1934).…”

Wolbachia infection status and molecular diversity in the species of tribe Tagiadini Mabille, 1878 (Lepidoptera: Hesperiidae) collected in China

“…Furthermore, we analysed the Wolbachia recombination and transmission patterns across Tagiadini species and revealed the potential effects of Wolbachia on variation and evolution of mtDNA. While Wolbachia has been investigated in many lepidopteran insects with a particular focus on infection status, sequence information, recombination or transmission patterns (Andersen et al., 2019 ; Arif et al., 2021 ; Bereczki et al., 2015 ; Dincă et al., 2019 ; Gaunet et al., 2019 ; Gislaine et al., 2018 ; Hinojosa et al., 2019 ; Jiang et al., 2014 , 2016 , 2018 ; Silva‐Brandão et al., 2021 ; Śliwińska et al., 2019 ; Yudina et al., 2016 ; Zhang et al., 2021 ), there are few studies that focus on the inter‐specific level across a large area. This has led us to complete a comprehensive survey of Wolbachia across the Tagiadini.…”