Wolbachia significantly impacts the vector competence of Aedes aegypti for Mayaro virus

Pereira, Thiago Nunes; Rocha, Marcele Neves; Sucupira, Pedro Henrique Ferreira; Carvalho, Fabiano Duarte; Moreira, Luciano Andrade

doi:10.1038/s41598-018-25236-8

Cited by 67 publications

(81 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Aedes aegypti can transmit a number of medically important arboviruses, including dengue, Zika, chikungunya, Mayaro and yellow fever viruses [1][2][3][4][5]. Together, these viruses cause significant morbidity and mortality across the world, with an estimated 100 million people experiencing a symptomatic dengue virus (DENV) infection each year [6].…”

Section: Introductionmentioning

confidence: 99%

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

et al. 2020

View full text Add to dashboard Cite

The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachiainfected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbBinfected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/ absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential.

show abstract

Section: Introductionmentioning

confidence: 99%

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

et al. 2020

View full text Add to dashboard Cite

show abstract

“…aegypti was nanoinjected into naïve mosquitoes, with 69% of mosquitoes becoming infected. Other laboratory studies also confirm the ability of this vector to transmit MAYV(14,16,34). Therefore, our results confirm that this vector has a great potential for infection and transmission of MAYV and therefore, play an important role in the transmission of this virus, if it gets urbanized.…”

Section: Discussionmentioning

confidence: 62%

“…In general, these mosquitoes presented high levels of infection in heads+thoraces at 14 dpi, averaging 9.6×10 4 and 4.7×10 4 viral particles, for replicates A and B, respectively. A previous study by Pereira et al ., 2018(34), showed that Ae. aegypti mosquitoes from Rio de Janeiro were highly susceptible to MAYV, presenting a higher number of viral copies than those obtained in this study.…”

Section: Discussionmentioning

confidence: 99%

“…In relation to the Ae. aegypti mosquito, Pereira et al ., 2018(34), observed that when these mosquitoes harbor Wolbachia they had significant impaired ability to infect/transmit MAYV. This strategy could become an effective tool for reducing MAYV transmission, throughout Latin America.…”

Section: Discussionmentioning

confidence: 99%

“…MAYV infection/dissemination in mosquitoes was quantified by RT-qPCR using LightCycler® 96 (Roche). A multiplex assay was performed according to previous studies(14,33,34). All samples were tested in duplicate for MAYV and the viral titer was determined through standard curve using serial dilutions of the target gene cloned into the pGEMT-Easy plasmid (Promega).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus

Moreira

Pereira

Carvalho

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

AbstractNewly emerging or re-emerging arthropod-borne viruses (arboviruses) are important causes of human morbidity and mortality nearly worldwide. Arboviruses such as Dengue (DENV), Zika (ZIKV), Chikungunya (CHIKV) and West Nile virus (WNV) underwent an extensive geographic expansion in the tropical and sub-tropical regions of the world. In the Americas the main vectors, for DENV, ZIKV and CHIKV, are mosquito species adapted to urban environments namely Aedes aegypti and Aedes albopictus, whereas the main vector for WNV is the Culex quinquefasciatus. Given the widespread distribution in the Americas and high permissiveness to arbovirus infection, theses mosquito species might pose an important role in the epidemiology of other arboviruses normally associated to sylvatic vectors. Here, we test this hypothesis by determining the vector competence of Ae. aegypti, Ae. albopictus and Cx. quinquefasciatus to Mayaro (MAYV) virus, a sylvatic arbovirus transmitted mainly by Haemagogus janthinomys that have been causing an increasing number of outbreaks in South America namely in Brazil. Using field mosquitoes from Brazil, female mosquitoes were experimentally infected and their competence for dissemination and transmission for MAYV was evaluated. We found high dissemination rate for MAYV in Ae. aegypti (57.5%) and Ae. albopictus (61.6%), whereas very low rates were obtained for Cx. quinquefasciatus (2.5%). Concordantly, we observed that Ae. aegypti and Ae. albopictus have high transmission ability (69.5% and 71.1% respectively), conversely to Cx. quinquefasciatus that is not able to transmit the MAYV. Notably, we found that very low quantities of virus present in the saliva (undetectable by RT-qPCR) were sufficient and virulent enough to guarantee transmission. Although Ae. aegypti and Ae. albopictus mosquitoes are not the main vectors for MAYV, our studies suggest that these vectors may play a significant role in the transmission of this arbovirus, since both species showed high vector competence in laboratory conditions.Author summaryThe present study showed that Ae. aegypti and Ae. albopictus mosquitoes have high vector competence for MAYV, in laboratory. In contrast, Cx. quinquefasciatus mosquitoes were shown to be refractory to MAYV. Regarding the viral dilution and nanoinjection, higher detection sensitivity was observed after virus nanoinjection into naïve mosquitoes, indicating that only a few viral particles are required to infect mosquitoes, and these particles may not be detected by RT-qPCR before the nanoinjection procedure.

show abstract

Recently introducedWolbachiareduces bacterial species richness and reshapes bacterial community structure inNilaparvata lugens

Zhou

Gong

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Wolbachia has been developed as an effective tool to suppress insect pests and arbovirus transmission. Recently, the brown planthopper Nilaparvata lugens, a serious agricultural pest, has been successfully transinfected with Wolbachia wStri strain from Laodelphax striatellus. However, before conducting the field experiments, the impacts of wStri on the bacterial microbiota in N. lugens and how it differs from native Wolbachia wLug strain have not been clarified. RESULTS Here, we found that wStri reduced bacterial diversity and shaped bacterial community structure more than wLug in both developmental stage and different adult tissues. Overall, the relative abundance of Wolbachia was negatively correlated with bacterial diversity, but the bacterial diversity gradually decreased only when the relative abundance of Wolbachia was higher than 60%. Further analysis found that wStri reduced species richness of other bacteria but not their evenness. wStri infection also affected many bacterial functions (e.g., amino acid metabolism & signaling and cellular processes) in the developmental stages, with a stronger effect than wLug in nymphs. Moreover, although Wolbachia occupied a high relative abundance in infected individuals, Acinetobacter was consistently a core part of microbiome. CONCLUSION These results showed the significant impacts of recently introduced wStri on bacterial microbiota in N. lugens, with the effects differing from native wLug. This study will aid in understanding the relationship between Wolbachia, its host and the host’s microbiota, and provide a reference for future field experiments.

show abstract

Wolbachia significantly impacts the vector competence of Aedes aegypti for Mayaro virus

Cited by 67 publications

References 43 publications

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus

Recently introducedWolbachiareduces bacterial species richness and reshapes bacterial community structure inNilaparvata lugens

Contact Info

Product

Resources

About