This paper provides evidence of gender differences in the access and control of resources and their relation to participation in rice-breeding activities among men and women farmers in southern Ghana. We used a mixed methods design which involved the use of qualitative data collected through focus group discussions (FGDs) and key informant interviews (KIIs) and quantitative data collection through a survey. Using data collected from 315 smallholder rice farmers, perception analyses and probit and multivariate regression were employed in the analyses. Our findings indicate that higher levels of education, experience in rice farming, a favorable dependency ratio, larger farm size, more rice plots, access to extension services, and involvement with financial organizations positively influence participation in rice-breeding activities. On the other hand, distance to market is found to have a negative impact on participation. Moreover, years of education, experience in rice farming, farm size, number of rice plots, dependency ratio, and distance to market were found to negatively influence the control of production resources among both male and female participants in rice-breeding activities. From both the quantitative and qualitative results, men had more access to productive resources than women. Insights from this study will enhance gender equity in promoting the participation of both men and women in rice varietal development activities.