Increasing numbers of women are entering the once male-dominated dental profession in Australia. Determining what differences exist in the practice of dentistry between male and female dentists has therefore become an important task. The purpose of the present study was to examine differences in practice between male and female dentists. Data were available from a weighted, stratified random sample of 730 dentists (486 male and 294 female dentists) in each State or Territory of Australia. Using discriminant analysis age, practice setting, number of other dentists in the practice, inputs to the practice of dentistry either hired, acquired or contributed, direct demand and community size were all found significant in separating male and female dentists. It was more difficult to separate younger male and female dentists, indicating some convergence between the sexes. However, some differences persisted. Possible reasons for the differences are discussed and some implications noted.