The demand of the contemporary society for renewable energy sources lead to the increase of the bio-power plants. Accordingly, the amount of ash generated by burning the biomass is increased, and its disposal becomes a large environmental problem. The paper presents the research of potential use of biomass wood ash as a partial replacement for coal fly ash (10%, 20%, 30% and 40% of mass) in production of self-compacting concrete (SCC). The effects of biomass wood ash on the properties of SCC in fresh and hardened states have been examined, as well as on the properties of durability. Test results indicated that the biomass wood ash slightly reduces the flowability and passing ability of SCC, while its addition enhances the viscosity of SCC and significantly prevents segregation and bleeding. SCCs with the contents of biomass wood ash up to 20% have approximately same mechanical strength as the reference mixture. Biomass wood ash has no negative effect on the resistance of concrete to the action of water under pressure, but a decrease of freeze/thaw resistance with de-icing salt is detected as its contents increases. The addition of biomass wood ash into SCC increases the drying shrinkage in the initial period of drying (up to 14 days), and it is decreased in a later phase.