Wooden breast lesions in broiler chickens in the UK

Brot, Simone de; Perez, Sara; Shivaprasad, H. L.; Baiker, Kerstin; Polledo, L.; Clark, Michael; Grau‐Roma, Llorenç

doi:10.1136/vr.103561

Cited by 26 publications

(20 citation statements)

References 9 publications

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“…WB incidence has increased drastically from an average of 5% in 2012 to 29% in 2015 and has been reported to affect up to 50% in other flocks (8). WB myopathy is emerging on a global scale, already present in Finland (9), France (10), Italy (5), Spain (11), Brazil (12,13), United Kingdom (14), Japan (15), and in several other countries, and it is negatively impacting global chicken meat production and quality.…”

Section: Introductionmentioning

confidence: 99%

Muscle Metabolome Profiles in Woody Breast-(un)Affected Broilers: Effects of Quantum Blue Phytase-Enriched Diet

et al. 2020

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Woody breast (WB) myopathy is significantly impacting modern broilers and is imposing a huge economic burden on the poultry industry worldwide. Yet, its etiology is not fully defined. In a previous study, we have shown that hypoxia and the activation of its upstream mediators (AKT/PI3K/mTOR) played a key role in WB myopathy, and supplementation of quantum blue (QB) can help to reduce WB severity via modulation of hypoxia-related pathways. To gain further insights, we undertook here a metabolomics approach to identify key metabolite signatures and outline their most enriched biological functions. Ultra performance liquid chromatography coupled with high resolution mass spectrometry (UPLC-HRMS) identified a total of 108 known metabolites. Of these, mean intensity differences at P < 0.05 were found in 60 metabolites with 42 higher and 18 lower in WB-affected compared to unaffected muscles. Multivariate analysis and Partial Least Squares Discriminant analysis (PLS-DA) scores plot displayed different clusters when comparing metabolites profile from affected and unaffected tissues and from moderate (MOD) and severe (SEV) WB muscles indicating that unique metabolite profiles are present for the WB-affected and unaffected muscles. To gain biologically related molecule networks, a stringent pathway analyses was conducted using IPA knowledge-base. The top 10 canonical pathways generated, using a fold-change −1.5 and 1.5 cutoff, with the 50 differentially abundant-metabolites were purine nucleotide degradation and de novo biosynthesis, sirtuin signaling pathway, citrulline-nitric oxide cycle, salvage pathways of pyrimidine DNA, IL-1 signaling, iNOS, Angiogenesis, PI3K/AKT signaling, and oxidative phosphorylation. The top altered bio-functions in term of molecular and cellular functions in WB-affected tissues included cellular development, cellular growth and proliferation, cellular death and survival, small molecular biochemistry, inflammatory response, free radical scavenging, cell signaling and cell-to-cell interaction, cell cycles, and lipid, carbohydrate, amino acid, and nucleic acid metabolisms. The top disorder functions identified were organismal injury and abnormalities, cancer, skeletal and muscular disorders, connective tissue disorders, and inflammatory diseases. Greene et al. Quantum Blue and Metabolome in Broilers Breast tissues from birds fed with high dose (2,000 FTU) of QB phytase exhibited 22 metabolites with significantly different levels compared to the control group with a clear cluster using PLS-DA analysis. Of these 22 metabolites, 9 were differentially abundant between WB-affected and unaffected muscles. Taken together, this study determined many metabolic signatures and disordered pathways, which could be regarded as new routes for discovering potential mechanisms of WB myopathy.

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Section: Introductionmentioning

confidence: 99%

Muscle Metabolome Profiles in Woody Breast-(un)Affected Broilers: Effects of Quantum Blue Phytase-Enriched Diet

et al. 2020

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“…The phenotypic stiffness of WB occurs in varying degrees and significantly affects quality traits and functional properties of broiler breast muscle ( Mudalal et al., 2015 ; Soglia et al., 2016 ). Woody breast has emerged on a global scale with documented occurrences in the United States ( Kuttappan et al., 2016 ), Finland ( Sihvo et al., 2014 ), Italy ( Mudalal et al., 2015 ), the United Kingdom ( de Brot et al., 2016 ), and in many other countries, with incidences reportedly affecting broilers from 30 to 50% that are grown for 8 wk to a live body weight greater than 4.2 kg. The myopathy constitutes a major animal health, welfare, and economic concern and is estimated to cost the US poultry industry more than 200 million dollars a year because of on-farm culling and mortality, downgrading, and condemnation at processing, as well as rejection from human consumption ( Kuttappan et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Research Note: Dietary phytase reduces broiler woody breast severity via potential modulation of breast muscle fatty acid profiles

et al. 2020

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Woody breast ( WB ) myopathy is a major concern and economic burden to the poultry industry, and for which, there is no effective solution because of its unknown etiology. In a previous study, we have shown that phytase (Quantum Blue, QB ) reduces the WB severity by 5% via modulation of oxygen homeostasis-related pathways. As WB has been suggested to be associated with lipid dysmetabolism, we aimed to determine the effect of QB on WB and breast muscle fatty acid profile. Male broilers were subjected to 6 treatments (96 birds/treatment): a nutrient adequate control group (PC), the PC supplemented with 0.3% myo-inositol (PC + MI), a negative control ( NC ) deficient in available P and Ca by 0.15 and 0.16%, respectively, the NC fed with QB at 500 (NC+500 FTU), and 1,000 (NC+ 1,000 FTU) or 2,000 FTU/kg of feed (NC+2,000 FTU). Woody breast and white striping scores were recorded, and fatty acid profiles were determined using gas liquid chromatography. Woody breast-affected muscles exhibited a significant higher incidence of white striping as liquid chromatography analysis reveals an imbalance of fatty acid profile in the breast of WB-affected birds with a significant higher percent of saturated fatty acids (SFA, myristic [14:0], pentadecanoic [15:0], and margaric [17:0]) and monounsaturated fatty acids (myristoleic [14:1], palmitoleic [16:1 c ], 10-trans-heptadecenoic [17:1 t ], oleic [18:1 c 9], and vaccenic [18:1 c 11]), and lower content of polyunsaturated fatty acids (PUFA) and omega-3 ( P < 0.05). Quantum Blue at high doses (1,000 and 2,000 FTU) significantly reduces the percent of SFA and increases that of PUFA compared with the control group. In conclusion, WB myopathy seemed to be associated with an imbalance of fatty acid profile, and QB ameliorates the severity of WB potentially via modulation of SFA and PUFA contents.

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“…These features are frequently accompanied by inflammatory cell infiltration into and around the myofibres, primarily heterophils and macrophages. Advanced cases are characterized by fibrosis, typified by thickened interstitial, endomysial and perimysial connective tissues and irregular deposition of adipose tissue (Sihvo et al, 2014;Velleman & Clark, 2015;de Brot et al, 2016;Sihvo et al, 2017). A rather unique histologic lesion frequently associated with this condition is phlebitis, characterized by the infiltration of mononuclear inflammatory cells into the walls of veins, whereas neighbouring arteries remain largely unaffected (Sihvo et al, 2014;Sihvo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Evidence and role of phlebitis and lipid infiltration in the onset and pathogenesis of Wooden Breast Disease in modern broiler chickens

et al. 2017

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Wooden Breast Disease (WBD), a myopathy that frequently affects modern broiler chickens, is a disorder that has been associated with significant economic losses in the poultry industry. To examine tissue changes associated with the onset and early pathogenesis of this disorder, a time-series experiment was conducted using chickens from a high-breast-muscle-yield, purebred commercial broiler line. Birds were raised for up to seven weeks, with a subset of birds sampled weekly. Breast muscle tissues were extracted at necropsy and processed for analysis by light microscopy and transmission electron microscopy. Histologic presentation indicated localized phlebitis with lipogranulomas in Week 1, focal single-myofibril degeneration in Week 2 preceding an inflammatory response that started in Week 3. Lesions in Week 4 were characterized by multifocal to diffuse muscle fibre degeneration, necrosis, interstitial oedema accompanied by increased lipid and inflammatory cell infiltration. Lesions in Weeks 5-7 revealed diffuse muscle degeneration, necrosis, fibrosis and fatty infiltration with lipogranulomas. Ultrastructural examination showed myofibrillar splitting and degeneration, irregular, displaced and degenerated Z-lines, mitochondrial degeneration and interstitial fibrosis with dense regular collagen fibres. This study, therefore, demonstrates that WBD exhibits an earlier onset in modern broilers than when detectable by clinical examination. Further, this study shows that the disease assumes a progressive course with acute vasculitis, lipid deposition and myodegeneration occurring in the earlier stages, followed by a chronic fibrotic phase.

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Wooden breast lesions in broiler chickens in the UK

Abstract: 20A new condition causing significant economic losses due to lesions in the pectoralis major 21 muscle of commercial broiler chickens has been recently reported in Finland. The condition 22

Cited by 26 publications

References 9 publications

Muscle Metabolome Profiles in Woody Breast-(un)Affected Broilers: Effects of Quantum Blue Phytase-Enriched Diet

Muscle Metabolome Profiles in Woody Breast-(un)Affected Broilers: Effects of Quantum Blue Phytase-Enriched Diet

Research Note: Dietary phytase reduces broiler woody breast severity via potential modulation of breast muscle fatty acid profiles

Evidence and role of phlebitis and lipid infiltration in the onset and pathogenesis of Wooden Breast Disease in modern broiler chickens

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