Work of adhesion between mucin macromolecule and calcium-alginate gels on molecular level

Popeski-Dimovski, Riste

doi:10.1016/j.carbpol.2015.01.033

Cited by 17 publications

(15 citation statements)

References 11 publications

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“…This situation holds up to the extreme 10 µm/s pulling, where the tip-mucin contact rupture takes place in an abrupt manner under application of lower forces. Overall, values remained in the range between 2 * 10 −18 and 2.5 * 10 −17 Joules (see complete set of values in TableSI1, Supporting Info) which agrees with results reported before for similar experiments 27 . The significance of the measured variations is found in the Supporting Information.…”

Section: Resultssupporting

confidence: 91%

“…70 µm/s) made extrapolation to our particular case just impossible. A measuring system resembling better -in terms of scale- our current study is that of Popeski-Dimovski 27 . However, experiments in that work were performed following an opposite approach: hydrophilic tips were coated with mucins, and then employed to measure their binding affinity to alginate-based hydrogels.…”

Section: Discussionmentioning

confidence: 99%

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Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH

Sumarokova

Iturri

Weber

et al. 2018

Sci Rep

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Mucins, the main component of the mucus secretions of goblet and epithelial cells, are known for exhibiting a different behaviour in accordance with their surrounding environment (i.e. among others the environmental pH), which induces a drastic change in their measured mechanical properties. In this work, we have first employed Atomic Force Microscopy (AFM) in Force Spectroscopy mode to evaluate the adhesion of porcine mucin films at the nanoscale, and the changes caused in this particular factor by a pH variation between 7.0 and 4.0, both quite common values in biological conditions. Measurements also involved additional varying factors such as the indenting tip chemistry (hydrophobic vs hydrophilic), its residence time on the measured film (0, 1 and/or 2 seconds), and increasing pulling rates (ranging from 0.1 up to 10 µm/s). A second approach regarded the macroscale behaviour of the films, due to their potential applicability in the development of a new set of stimuli-responsive biomaterials. This was possible by means of complementary Wilhelmy plate method (to test the wetting properties) and cell proliferation studies on films previously exposed to the corresponding pH solution. According to our results, treatment with lowest pH (4.0) provides porcine mucin with a more hydrophilic character, showing a much stronger adhesion for analogous chemistries, as well as enhanced capability for cell attachment and proliferation, which opens new pathways for their future use and consideration as scaffold-forming material.

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Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH

Sumarokova

Iturri

Weber

et al. 2018

Sci Rep

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“…Studying cell mechanics at the molecular level includes measuring the molecular interaction between a ligand and a receptor. After the pioneering work of Florin et al measuring the binding energy of avidin and biotin, some authors have repeated this type of experiment . However, coating a tip with a ligand and maintaining its functionality is challenging since the attachment needs to be oriented to expose the binding sites of the molecule at the tip apex and subsequently, most authors use polyethylene glycol linkers in order to provide freedom for the ligand and receptor to interact.…”

Section: Afm Studies Of Cell Mechanotransductionmentioning

confidence: 99%

Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

Kilpatrick

Revenko

Rodriguez

2015

Adv Healthcare Materials

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The behavior and mechanical properties of cells are strongly dependent on the biochemical and biomechanical properties of their microenvironment. Thus, understanding the mechanical properties of cells, extracellular matrices, and biomaterials is key to understanding cell function and to develop new materials with tailored mechanical properties for tissue engineering and regenerative medicine applications. Atomic force microscopy (AFM) has emerged as an indispensable technique for measuring the mechanical properties of biomaterials and cells with high spatial resolution and force sensitivity within physiologically relevant environments and timescales in the kPa to GPa elastic modulus range. The growing interest in this field of bionanomechanics has been accompanied by an expanding array of models to describe the complexity of indentation of hierarchical biological samples. Furthermore, the integration of AFM with optical microscopy techniques has further opened the door to a wide range of mechanotransduction studies. In recent years, new multidimensional and multiharmonic AFM approaches for mapping mechanical properties have been developed, which allow the rapid determination of, for example, cell elasticity. This Progress Report provides an introduction and practical guide to making AFM-based nanomechanical measurements of cells and surfaces for tissue engineering applications.

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“…Calcium alginate (CaA) is a typical example of hydrogels with stable “egg‐box” structure and it has been considered as an ideal candidate for protein imprinting. Nowadays, lots of researches have been attracted by CaA because of its excellent biocompatibility, nontoxicity, and low cost . However, alginate hydrogels easily erode and disintegrate because of ion exchanging .…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, lots of researches have been attracted by CaA because of its excellent biocompatibility, nontoxicity, and low cost. [13][14][15] How-ever, alginate hydrogels easily erode and disintegrate because of ion exchanging. 16 Many modifications including physical or chemical procedures towards alginates have been carried out and some remarkable improvements have been achieved.…”

Section: Introductionmentioning

confidence: 99%

BSA imprinted polyethylene glycol grafted calcium alginate hydrogel microspheres

Liu

Ying

Wang

et al. 2016

J of Applied Polymer Sci

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Polyethylene glycol grafted calcium alginate hydrogel microspheres are synthesized by ring‐opening and addition reaction of epoxy chloropropane. FTIR, 13C NMR, optical microscope, SEM, TG, and DSC are used for characterization. Swelling behaviors are studied and it is found that polyethylene glycol grafted calcium alginate microspheres are improved in anti‐swelling properties. Protein‐imprinted polyethylene glycol grafted calcium alginate hydrogel microspheres exhibit enhanced specific rebinding quantity (11.16 mg/g) and selective recognition properties (4.71 against BHb), towards target molecule in the presence of competing protein, compared with that of the unmodified samples (4.96 mg/g and 1.75, respectively). © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43617.

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Work of adhesion between mucin macromolecule and calcium-alginate gels on molecular level

Cited by 17 publications

References 11 publications

Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH

Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH

Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

BSA imprinted polyethylene glycol grafted calcium alginate hydrogel microspheres

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