Wound dressing with sustained anti-microbial capability

Abstract

To overcome current limitations in wound dressings for treating mustard-burn induced septic wound injuries, a nonadherent wound dressing with sustained anti-microbial capability has been developed. The wound dressing consists of two layers: the upper layer is a carboxymethyl-chitin hydrogel material, while the lower layer is an anti-microbial impregnated biomaterial. The hydrogel layer acts as a mechanical and microbial barrier, and is capable of absorbing wound exudate. In physiological fluid, the carboxymethylated-chitin hydrogel swells considerably, imbibing up to 4 times its own weight of water and is also highly porous to water vapor. The moisture permeability of the dressing prevents the accumulation of fluid in heavily exudating wounds seen in second-degree burns. The lower layer, fabricated from chitosan acetate foam, is impregnated with chlorhexidine gluconate. From the in vitro release studies, the loading concentration was optimized to deliver sufficient anti-microbial drug into the wound area to sustain the anti-microbial activity for 24 h. The anti-microbial activity of the dressing against Pseudomonas aeruginosa and Staphylococcus aureus was tested using the Bauer-Kirby Disk Diffusion Test.

Composite poly(vinyl alcohol)/poly(vinyl acetate) electrospun nanofibrous mats as a novel wound dressing matrix for controlled release of drugs

“…4,5 Furthermore, a proper wound dressing needs to have wound healing properties, including the essential swelling capability for absorbing excess exudates and oxygen permeability for respiring. 5,6 Modern dressings as vehicles for delivering therapeutic agents to wound sites have been applied in the form of hydrogels, 2 films, 7 sponges, 8 foams, 9 and recently in the form of nanofibers. 6,10 However, little research has been published on the controlled delivery of drugs from polymeric dressing, especially in the form of nanofibers.…”

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“…Даже внутри-венный способ введения не обеспечивает достаточной концентрации антибиотиков в ране на длительное время, из-за гемодинамических и морфологических изменений в патологическом очаге и окружающих тканях [18,22,46]. Понятно, что проблема раневой инфекции не может быть решена за счет новых лекарственных средств, так как основное значение в борьбе с хирургической инфекцией принадлежит защитным способностям самого организма, его реактивности и созданию ранних оптимальных условий для жизнедеятельности клеток и тканей [7,13,31,51,53].…”

“…Натриевые каналы изменяют электрические свойства клеток сердца, что обеспечивает начало сердечного со-кращения, кроме того, они координируют сокращения предсердий и желудочков и поддерживают нормальный ритм сердца. Мутации гена SCN5A приводят к возник-новению различных заболеваний сердечно-сосудистой системы, таких как семейные формы аритмий, синдром удлиненного интервала QT, синдром Бругада, прогресси-рующие расстройства сердечной проводимости, синдром внезапной детской смерти [27,53]. Доказано, что мутации гена SCN5A, приводящие к замене некоторых аминокислот натриевого канала, влияют на развитие синдрома Бругада и синдрома внезапной ночной сердечной смерти.…”

Novel Chitin and Chitosan Materials in Wound Dressing

“…However, chitin does not result in a higher metabolic rate (by MTT assays), suggesting that this polymeric material is not causing marked increases in cellular proliferation but is rescuing cells from dying by serum deprivation. To overcome current limitations in wound dressings for treating mustard-burn induced septic wound injuries, a non-adherent wound dressing with sustained anti-microbial capability has been developed (Loke et al, 2000). The wound dressing consists of two layers: the upper layer is a carboxymethyl chitin hydrogel material, while the lower layer is an anti-microbial impregnated biomaterial.…”

“…The lower layer, fabricated from chitosan acetate foam, is impregnated with chlorhexidine gluconate. From the in vitro release studies, the loading concentration was optimized to deliver sufficient anti-microbial drug into the wound area to sustain the anti-microbial activity for 24 h (Loke et al, 2000).…”