Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: an experimental animal study

Abstract

BackgroundThe efficacy of Centella asiatica for incision and burn wounds are not fully understood. Here, we report the wound healing activities of sequential hexane, ethyl acetate, methanol, and water extracts of Centella asiatica in incision and partial-thickness burn wound models in rats.MethodsMale Sprague–Dawley rats weighing 250–300 g were randomly divided into incision and burn wound groups. Each group was stratified into seven subgroups: (1) untreated; (2) NSS-; (3) Tween 20®- (vehicle control); (4) hexane extract-; (5) ethyl acetate extract-; (6) methanol extract-; and (7) aqueous extract-treated groups. The test substances were applied topically once daily. The tensile strength of the incision wound was measured on the seventh day after wound infliction. The general appearance and degree of wound healing of the burn wound were assessed on Days 3, 7, 10 and 14 after burn injury and prior to histopathological evaluation.ResultsOn the seventh day after wound infliction, the tensile strength of incision wound in all extract-treated groups was significantly higher than that of the vehicle control (Tween 20®), but comparable to the NSS-treated group. The degrees of healing in the burn wound with the four extracts were significantly higher than that of the control on Days 3, 10 and 14. Histopathological findings on Day 14 after burn injury revealed prominent fibrinoid necrosis and incomplete epithelialization in the control and untreated groups, whereas fully developed epithelialization and keratinization were observed in all extract-treated groups. Analysis by thin layer chromatography demonstrated that the phyto-constituents β-sitosterol, asiatic acid, and asiaticoside and madecassocide were present in the hexane, ethyl acetate and methanol extracts, respectively.ConclusionsAll extracts of Centella asiatica facilitate the wound healing process in both incision and burn wounds. Asiatic acid in the ethyl acetate extract seemed to be the most active component for healing the wound.

Botanical Pharmacognosy of Centella asiatica (Linn.)Urban

“…Data on many recent experimental studies have scientifically validated the traditional uses of the extract of Mandukaparni. These findings endorse that the plant having multiple therapeutic potentials, which were amply and vividly demonstrated by different animal models such as wound-healing model in rat, [10][11] streptozocin-induced Alzheimeir's model 12 , zebrafish Parkinson's model, 13 adriamycin-induced cardiomyopathy model, 14 nitroglycerine and bradykinin induced hyperalgesia (migraine) model, 15 radiationinduced dermatitis model, memory impairment induced by cerebral ischemia-reperfusion 16 and also having anxiolytic, 17 neuronal dendritic growth and nerve stimulating effects, 18 suppression of scars in diabetic patients, 19 and reverse mutation assay for non-toxicity, safety and nonmutagenicity of the extract, 20 protection of healthy cells against radiationinduced damages. 21 However, the botanical standards specified for evaluation of intact plant material of Centella asiatica in literature [22][23][24][25] seemed to be too scanty and not exhaustive enough to extricate the crude raw materials from the adulterants and substitutes.…”

Evaluation of Antioxidant, Toxicological and wound healing Properties of Hibiscus rosa-sinensis L. (Malvaceae) ethanolic leaves extract on different Experimental animal models

“…Few antimicrobial ointments such as silver sulfadiazine, mafenide, silver nitrate, povidone-iodine, mupirocin and bacitracin, are used to reduce the risk of infection in minor cuts and burns but these topical antimicrobials are not fully effective in treatment of wounds and may cause some side effects. 16 Thus there is need to develop safer drugs to combat different types of wounds. Literature available from all possible scientific sources revealed that the leaves of Hibiscus rosa-sinensis have not been evaluated for its toxicological effects and also its wound healing potential.…”

Effects of Prunus spinosa L. fruits on experimental wound healing

“…Yara iyileşmesini hızlandırmak ve düzenlemek amacıyla pek çok tıbbi ve geleneksel ilaç kullanımına dair halen çalışmalar yapılmakta ve yeni ürünler araştırılmaktadır. Etki mekanizmaları göz önünde bulundurulduğunda, hücre çoğalmasını, anjiogenezisi ve kollajen sentezini artırıcı ve epitelizasyonu hızlandırıcı pek çok ajan bu amaçla kullanılmakta veya araştırılmaktadır [24][25][26][27][28] . P. spinosa meyvelerinde, bir flavonoit çeşidi olan ve kardiyovasküler koruyucu, antiinflamatuvar ve antioksidan etkileri olan çeşitli antosiyaninler (siyanidin-3-rutinosit, peonidin-3-rutinozit, siyanidin-3-glikozit) bulunmaktadır 10 .…”