Wound Healing Activities of Hydromethanolic Crude Extract and Solvent Fractions of Bersama abyssinica Leaves in Mice

Abstract

Background. Bersama abyssinica leaves are traditionally used for management of wounds in several communities of Ethiopia, despite no scientifically approved studies done on wound healing. Our study planned to work out the wound healing effects of B. abyssinica leaves extract in mice. Methods. B. abyssinica leaves were extracted with 80% hydromethanol and fractioned with chloroform, hexane, and water. The acute dermal toxicity of the crude extract was evaluated in mice. The crude extract formulated at 5% and 10% w/w ointment was investigated in excision, incision, and burn wound models and solvent fractions in the excision model using simple ointment (negative control) and nitrofurazone 0.2% w/v (positive control). We evaluated histopathological analysis, wound contraction rate, complete epithelialization period, and skin durability. One-way ANOVA followed by the post hoc Tukey HSD test with IBM SPSS software version 23.0 was used for data analysis, and p < 0.05 was considered statistically significant. Results. Hydromethanolic crude extract produced 5% (99.5%) and 10% (100%) wound contraction on the 16th day of the treatment and 5% (18.8) and 10% (28.2) percent reduction in the epithelization period on the excision wound healing model. Hydromethanolic crude extract produced 5% (47.5) and 10% (61.17) percent durability on the incision wound healing model. Hydromethanolic crude extract produced 5% (99.82%) and 10% (100%) wound contraction on the 20th day of treatment and 5% (13%) and 10% (21.7%) reduction in the epithelization period on the burn wound healing model. The chloroform fraction produced 5% (90.17%) and 10% (91.01%), hexane fraction produced 5% (85.81%) and 10% (86.78%), and aqueous fraction produced 5% (99.17%) and 10% (99.38%) wound contraction on the 14th day of the treatment and 5% (18.8) and 10% (28.2) percent reduction in the epithelization period on the excision wound healing model. Both hydromethanolic crude extract and solvent fractions at 5% and 10% (w/w) were significant ( p < 0.001) compared with negative control. Conclusion. The results of this study showed that both 5% w/w and 10% w/w of 80% hydromethanolic crude extract and solvent fractions of B. abyssinica leaves have wound healing effects.

Effect of Ducrosia anethifolia methanol extract against methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms on excision wound in diabetic mice

“…The selection of two different concentrations was based on pilot studies and skin irritation studies. There are several studies on different plant extracts using the same concentrations ( Taddese et al., 2021 ; Tekleyes et al., 2021 ). The ointment in a suitable base was used to increase the stability, spreadability, and diffusion ( Kolhe et al., 2018 ).…”

Toksisitas Akut Dermal Ekstrak Tanaman Sebagai Bagian dalam Aspek Keamanan Obat Herbal Terstandar Topikal

“…Namun ketika suatu kasus dimana perbedaan itu diamati, diketahui bahwa tikus betina sedikit lebih sensitif [32]. Sementara itu, sebanyak 3 penelitian lain menggunakan mencit sebagai hewan ujinya [7,45,46]. Persyaratan hewan uji lainnya menurut OECD No.…”

“…Sekitar 35% dari pasar obat global tahunan berasal dari produk alami atau obat-obatan terkait; termasuk sumber dari tanaman (25%), diikuti oleh mikroorganisme (13%), dan hewan (3%) [5]. Secara tradisional, masyarakat telah menggunakan tanaman sebagai obat topikal baik dari bagian daun [6], kulit batang [7], kulit pohon [8], dan bunga [9]. Pengembangan obat herbal juga tidak lepas dari faktor efek samping berbahaya yang ditimbulkan oleh obat sintetik sehingga saat ini pengobatan komplementer dan alternatif, terutama obat herbal, telah mendapatkan lebih banyak perhatian dan juga menjadi populer [10].…”

Evaluation of Wound Healing Activity of 80% Methanol Root Crude Extract and Solvent Fractions of Stephania abyssinica (Dill. & A. Rich.) Walp. (Menispermaceae) in Mice

“…1,32 The wound-healing effect of steroids and phenolic compounds could be due to their antimicrobial and anti-inflammatory activity. 44 The fast wound contraction in excision and burn wound models by the extract could be related to greater collagen fibers, fibroblasts, and capillary blood cells as indicated by histological investigation. The fibroblasts are activated and to the wound site, which is essential for cell metabolism, particularly the synthesis of energy and necessary nutrients required for wound repair.…”

“…This release promotes the absorption of the AQF contents, resulting in a faster rate of wound healing and a shorter epithelialization period. 44 The minimal wound contraction, longer period of epithelialization, more inflammatory cells, and low level of collagen, fibroblast, and new blood cell (angiogenesis) in the hexane fraction, possibly due to differences or low concentration of secondary metabolites or low amount of non-polar constituents present. Besides the slower release of…”