Wound healing activity of ent-kaura-9(11),16-dien-19-oic acid isolated from Wedelia trilobata (L.) leaves

Balekar, Neelam; Nakpheng, Titpawan; Gangadhar, Katkam N.; Srichana, Teerapol

doi:10.1016/j.phymed.2012.07.014

Cited by 42 publications

(14 citation statements)

References 30 publications

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“…Fractions that showed identical behaviour in thin layer chromatography (TLC) were combined. The pre-coated TLC plates developed with n-hexane, chloroform and ethyl acetate (98:20:2 v/v) showed a violet spot at a R f value of 0.52 when sprayed with anisol-sulphuric acid reagent and heated at 110 ºC for 5 minutes 22 . White crystals deposited on the walls after further purification of fraction.…”

Section: Methodsmentioning

confidence: 99%

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2017

IJPSR

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Section: Methodsmentioning

confidence: 99%

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2017

IJPSR

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“…The cytotoxicity test was evaluated by a cell proliferation and viability assay using the HaCaT and BJ cell lines. 15 The cells (2 × 10 4 cells/mL) were seeded into the wells of a 96 wells plate with their respective medium. Various concentrations (7.75, 15, 31, 62 and 124 µg/mL) of J. curcas latex extract, JcF and CAF were added to the wells, and incubated at 37 ºC for 24 h with 5 % CO2.…”

Section: Antioxidant Activitymentioning

confidence: 99%

In Vitro Studies of Jatropha Curcas L. Latex Spray Formulation for Wound Healing Applications

TINPUN¹,

NAKPHENG²,

PADMAVATHI³

et al. 2020

tjps

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Objective: There is an increasing demand for wound healing products of natural origin. Our objective is to develop a spray formulation from Jatropha curcas L. latex extracts for wound healing applications. Materials and Methods: J. curcas L. latex was subjected to solvent extraction. The phytochemical structure was elucidated by 1 H-NMR and confirmed by LC-MS spectrometry. A topical spray formulation prepared from J. curcas latex extracts were evaluated in antimicrobial activity and radical scavenging activity. The toxicity of formulation on fibroblast cell line, collagen production and wound healing activities were carried out. Results: The 1 H-NMR and mass spectrometric analyses revealed pure compound as curcacycline A. The J. curcas latex extract formulation had radical scavenging activity and antibacterial activities. Moreover, the formulation was not toxic to the human fibroblast cells while it stimulated collagen production and healed cell injury in 24 h. Conclusion: The J. curcas latex extract promoted the wound healing after cell injury. Our findings could rise the possibility of utilizing the J. curcas latex extract spray formulation as a potential the antibacterial, antioxidant and wound healing product from nature.

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“…In vitro cytotoxicity and wound healing activity We assessed the wound healing activity by using a well-established in vitro cell scratch assay, which is a widely used method to assess the wound healing activity of the plant extracts. [40][41][42][43][44] To the best of our knowledge, this is the first report regarding the wound healing activity of the species studied. Before performing wound healing assay, we investigated the potential cytotoxic effect of the extracts on L929 mouse fibroblast cell line since reduced levels of cell proliferation may affect the results.…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

İğci¹,

Aytaç²

2020

tjps

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GİRİŞ ve AMAÇ: Bu çalışma, Hypericum pseudolaeve Robson türünün iki farklı yöntemle elde edilmiş metanol ve su ekstrelerinin in vitro yara iyileştirici etkisi ile sitotoksisitesi, antioksidan aktivitesi ve seçilmiş fitokimyasallarını araştırmayı amaçlamıştır. YÖNTEM ve GEREÇLER: Toplam fenolik ve flavonoit içerikleri spektrofotometri tabanlı yöntemler kullanılarak ölçülmüştür. Ekstrelerin L929 fare fibroblast hücreleri üzerindeki sitotoksik etkileri MTT deneyi ile değerlendirilmiştir. Ayrıca, bitki uygulanmış fibroblast hücrelerinin göçü, bir in vitro yara iyileşme modeli olan hücre çizik yöntemi ile değerlendirilmiştir. Ek olarak, türün kimyasal içeriği Yüksek Performanslı Sıvı Kromatografisi (YPSK) ile belirlenmiştir. BULGULAR: Sitotoksisite deneyinin sonuçları, metanol ve su ekstrelerinin, 500 µg/mL konsantrasyonuna kadar kullanıldığında fibroblast hücreleri üzerinde herhangi bir sitotoksik etkiye sahip olmadığını göstermiştir. Fibroblast göçü 62 µg/mL konsantrasyonunda su ekstreleri uygulandığında negatif kontrole göre anlamlı derecede artış göstermiştir. Ekstreler iyi derecede antioksidan aktivite göstermiştir ve YPSK analizi ile 16 madde tespit edilmiştir. En fazla bulunan bileşik epikateşin olarak belirlenmiştir. TARTIŞMA ve SONUÇ: Bu çalışma Hypericum pseudolaeve ekstrelerinin, yara iyileşme potansiyeline sahip olduğunu ve çeşitli önemli antioksidan fenolikler içerdiğini göstermiştir. Bu türle ilgili aktif bileşiklerin izole edilmesi ve tanımlamasını amaçlayan daha fazla araştırma yapılmalıdır. Anahtar Kelimeler: Yara iyileşmesi, bitki ekstresi, antioksidan, fenolikler, YPSK INTRODUCTION: This study was aimed to investigate the in vitro wound healing effects of the methanolic and aqueous extracts of Hypericum pseudolaeve Robson obtained by two different methods as well as its cytotoxicity, antioxidant activity and selected phytochemical constituents. METHODS: Total phenolic and flavonoid contents were measured using spectrophotometry based methods. Cytotoxic effects of the extracts on L929 mouse fibroblast cells were evaluated by MTT assay. Moreover, migration and spreading of the treated fibroblast cells were assessed by cell scratch assay as an in vitro wound healing model. In addition, the chemical content of the species was determined by High Performance Liquid Chromatography (HPLC). RESULTS: The results of cytotoxicity assay indicated that the methanolic and aqueous extract did not have any cytotoxic effect on fibroblast cells at concentrations up to 500 µg/mL. Fibroblast migration was significantly increased by 62 µg/mL concentration of the aqueous extracts compared to negative control. Extracts showed good antioxidant activity and 16 phytochemical compounds were detected by HPLC, with the highest amount for u n c o r r e c t e d p r o o f 2 epicatechin. DISCUSSION AND CONCLUSION: Our results showed that H. pseudolaeve extracts have wound healing potential and contain several important antioxidant phenolic compounds. This species deserve further investigation aiming to isolate and identify the activ...

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Wound healing activity of ent-kaura-9(11),16-dien-19-oic acid isolated from Wedelia trilobata (L.) leaves

Cited by 42 publications

References 30 publications

Untitled

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In Vitro Studies of Jatropha Curcas L. Latex Spray Formulation for Wound Healing Applications

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

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