Wound Healing in Aged Normal and Ovariectomized Rats: Effects of Chemically Modified Doxycycline (CMT‐8) on MMP Expression and Collagen Synthesis

Ramamurthy, N. S.; McClain, Steve A.; Pirilä, Emma; Maisi, Päivi; Salo, Tuula; Kucine, Allan; Sorsa, Timo; Vishram, F.; Golub, Lorne M.

doi:10.1111/j.1749-6632.1999.tb07772.x

Cited by 19 publications

(15 citation statements)

References 4 publications

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“…This has led to the speculation that doxycycline or other tetracycline derivatives might have a use in the treatment of chronic wounds 25. In this study, the ability of doxycycline to exchange onto the S‐SEBS backbone and its subsequent release was examined.…”

Section: Resultsmentioning

confidence: 99%

Novel sulfonated hydrogel composite with the ability to inhibit proteases and bacterial growth

Vachon¹,

Yager

2005

J Biomedical Materials Res

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There is a growing interest in the development of wound dressings that possess functionality beyond providing physical protection and an optimal moisture environment for the wound. To this end, a novel dressing material based on a sulfonated triblock polymer has been developed. This versatile polymer possesses an ion-exchange capability that is amenable to binding and controlled release of a variety of therapeutic agents. This sulfonated polymer offers several advantages over existing commercial hydrogels used as wound dressings. These include (1) hydrophilicity that is proportional to sulfonation level, (2) easy preparation of fabric supported dressings (e.g., polyester, cotton, nylon), (3) excellent mechanical integrity of the materials when hydrated, (4) stability to a variety of chemistries, and (5) stability to a variety of sterilization methodologies. In this study, polymer was coated onto a polyester fabric and then modified by ion exchange to prepare the sodium, silver, or doxycycline salts. These sulfonated triblock polymer formulations were then evaluated for their capacity to sequester the neutrophil proteases, elastase, and collagenase-2 (MMP-8). Several of the formulations were found to sequester significant amounts of either elastase or collagenase. These formulations were demonstrated to be tested against a commercially available dressing that is currently marketed for its protease-inhibiting capability. The experimental dressing was statistically superior to the commercial dressing at inhibiting MMP-8 and elastase under the same conditions.

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Section: Resultsmentioning

confidence: 99%

Novel sulfonated hydrogel composite with the ability to inhibit proteases and bacterial growth

Vachon¹,

Yager

2005

J Biomedical Materials Res

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“…TTC inhibits extra-cellular MMP possibly due to its chelator properties on Ca 2 þ and Zn 2 þ ions (Golub et al 1987). TTC and chemically modified TTC (CMT) inhibit extracellular conversion of latent pro-MMP into activated MMP (Golub et al 1987;Ramamurthy et al 1999), and may enhance bone wound healing by other mechanisms. In diabetic rodent models (Sasaki et al 1992;Bain et al 1997), systemic TTC promoted bone formation up-regulating collagen and protein synthesis by osteoblasts.…”

mentioning

confidence: 99%

Systemic tetracycline delays degradation of three different collagen membranes in rat calvaria

Moses

Shemesh²,

Aboodi³

et al. 2009

Clinical Oral Implants Res

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“…Also, systemic DX had bone-sparing effects after periradicular surgery in dogs (17). Additionally, chemically modified DX (CMT-8), that does not display antimicrobial properties, reduced MMP expression in ovariectomized rats (18) and the same CMT-8, combined with disodium clodronate (an aminobisphosphonate that inhibits bone resorption), reduced alveolar bone loss in endotoxin-induced periodontal disease in rats (19).…”

mentioning

confidence: 99%

Low-dose doxycycline prevents inflammatory bone resorption in rats

Bezerra

Brito

Ribeiro

et al. 2002

Braz J Med Biol Res

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Matrix metalloproteinases (MMP) are considered to be key initiators of collagen degradation, thus contributing to bone resorption in inflammatory diseases. We determined whether subantimicrobial doses of doxycycline (DX) (£10 mg kg -1 day -1 ), a known MMP inhibitor, could inhibit bone resorption in an experimental periodontitis model. Thirty male Wistar rats (180-200 g) were subjected to placement of a nylon thread ligature around the maxillary molars and sacrificed after 7 days. Alveolar bone loss (ABL) was measured macroscopically in one hemiarcade and the contralateral hemiarcade was processed for histopathologic analysis. Groups of six animals each were treated with DX (2.5, 5 or 10 mg kg -1 day -1 , sc, 7 days) and compared to nontreated (NT) rats. NT rats displayed significant ABL, severe mononuclear cell influx and increase in osteoclast numbers, which were significantly reduced by 5 or 10 mg kg -1 day -1 DX. These data show that DX inhibits inflammatory bone resorption in a manner that is independent of its antimicrobial properties.

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Wound Healing in Aged Normal and Ovariectomized Rats: Effects of Chemically Modified Doxycycline (CMT‐8) on MMP Expression and Collagen Synthesis

Cited by 19 publications

References 4 publications

Novel sulfonated hydrogel composite with the ability to inhibit proteases and bacterial growth

Novel sulfonated hydrogel composite with the ability to inhibit proteases and bacterial growth

Systemic tetracycline delays degradation of three different collagen membranes in rat calvaria

Low-dose doxycycline prevents inflammatory bone resorption in rats

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