Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

El-Aassar, M. R.; Ibrahim, Omar; Fouda, Moustafa M.G.; El-Beheri, Nagham G.; Agwa, Mona M.

doi:10.1016/j.carbpol.2020.116175

Cited by 202 publications

(108 citation statements)

References 49 publications

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“…Using double sided adhesive tape, the recovered AgNPs were prepared by placing 5 μL of the colloid solution on a carbon coated 3-mm copper grid, making a thin film of sample on the grid and extra sample was removed by filter paper and dried at room temperature. ImageJ software was used for analysis and poly dispersity index was calculated according to the following equation (El-Aassar et al, 2020 ; Ibrahim et al, 2020 ):…”

Section: Methodsmentioning

confidence: 99%

Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa

El‐Deeb

Abo-Eleneen

Al-Madboly

et al. 2020

Front. Bioeng. Biotechnol.

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Bacterial infections are the key cause of death in patients suffering from burns and diabetic wounds while the use of traditional antibiotics has been growing steadily. Thus, in the present study, we are trying to introduce a paradigm shift strategy to improve chronic wound healing of bacterial infection. To that end, we have biologically synthesized silver nanoparticles (AgNPs) using Arthrospira sp polysaccharides, and evaluated their antibacterial efficacy with their safety pattern. Scanning electron micrographs showed spherical AgNPs coated with algal polysaccharides with an approximate size of 9.7 nm. Treatment of Pseudomonas aeruginosa with the AgNPs (0.5–1 μg/mL) resulted in a significant disruption in P. aeruginosa outer membrane, reduction in biofilm formation, and a significant decrease of production of alginate and pyocyanin along with a concentration-dependent reduction in β-lactamase activity. In addition, at the in vivo level, AgNPs displayed substantial activity to control P. aeruginosa infections in rat skin wounds with significant reduction in in COX-2 enzyme in both rat skin homogenate and serum samples. Furthermore, AgNPs facilitated wound curative in the P. aeruginosa infected model by reducing the hemorrhagic areas number and the infiltrated inflammatory cells. Taken all together, these biogenic nanoparticles showed unique properties in controlling bacterial wound infections and improving the healing process of damaged tissues via its direct and indirect effects.

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Section: Methodsmentioning

confidence: 99%

Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa

El‐Deeb

Abo-Eleneen

Al-Madboly

et al. 2020

Front. Bioeng. Biotechnol.

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“…The in vivo study in albino rats showed maximum wound epithelization and collagen deposition after 14 days of nanofiber administration [93].…”

Section: Chemical Methods (Nabh 4 ) 5-17 Alginatementioning

confidence: 94%

“…Successful AgNPs synthesis by PGA was verified by UV-vis spectral maxima ranging between 410 and 415 nm and a transmission electron microscopy image of 8.6 nm average particulate diameter. AgNPs components of (Ag-PGA/HA)-PVA nanofiber exhibited robust zone inhibition antibacterial activity against both Gram-positive and Gram-negative bacteria [93].…”

Section: Green Synthesis Of Agnps-containing Nanofibersmentioning

confidence: 99%

Silver Nanomaterials for Wound Dressing Applications

et al. 2020

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Silver nanoparticles (AgNPs) have recently become very attractive for the scientific community due to their broad spectrum of applications in the biomedical field. The main advantages of AgNPs include a simple method of synthesis, a simple way to change their morphology and high surface area to volume ratio. Much research has been carried out over the years to evaluate their possible effectivity against microbial organisms. The most important factors which influence the effectivity of AgNPs against microorganisms are the method of their preparation and the type of application. When incorporated into fabric wound dressings and other textiles, AgNPs have shown significant antibacterial activity against both Gram-positive and Gram-negative bacteria and inhibited biofilm formation. In this review, the different routes of synthesizing AgNPs with controlled size and geometry including chemical, green, irradiation and thermal synthesis, as well as the different types of application of AgNPs for wound dressings such as membrane immobilization, topical application, preparation of nanofibers and hydrogels, and the mechanism behind their antimicrobial activity, have been discussed elaborately.

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“…Hyaluronic acid creates a viscous matrix in the ECM within which elastin and collagen fibers are incorporated [ 6 ]. Due to its good biodegradability, biocompatibility, high degree of wettability, non-immunogenic character, and ability to be chemically modified, HA has important bio-applications in the fields of wound healing, tissue engineering, drug delivery, and visco-supplementation [ 153 ].…”

Section: Electrospun Nanofibers In Wound Healingmentioning

confidence: 99%

“…Another direction of the research consists of the inclusion of metallic nanoparticles (silver) to formulate a wound dressing nanofiber scaffold, containing biologically adsorbent materials. For this formulation, the silver nanoparticles will act as an anti-inflammatory and antioxidant that defend the cells from the devastating effect of raised amounts of reactive oxygen species (ROS) and in the same time, will facilitate wound healing process [ 153 ]. Also, recent researches in this field with the formulation of eNFs with application in wound management are highlighted in Table 6 .…”

Section: Electrospun Nanofibers In Wound Healingmentioning

confidence: 99%

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

et al. 2020

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Currently, despite the thoroughgoing scientific research carried out in the area of wound healing management, the treatment of skin injuries, regardless of etiology remains a big provocation for health care professionals. An optimal wound dressing should be nontoxic, non-adherent, non-allergenic, should also maintain a humid medium at the wound interfacing, and be easily removed without trauma. For the development of functional and bioactive dressings, they must meet different conditions such as: The ability to remove excess exudates, to allow gaseous interchange, to behave as a barrier to microbes and to external physical or chemical aggressions, and at the same time to have the capacity of promoting the process of healing by stimulating other intricate processes such as differentiation, cell adhesion, and proliferation. Over the past several years, various types of wound dressings including hydrogels, hydrocolloids, films, foams, sponges, and micro/nanofibers have been formulated, and among them, the electrospun nanofibrous mats received an increased interest from researchers due to the numerous advantages and their intrinsic properties. The drug-embedded nanofibers are the potential candidates for wound dressing application by virtue of: Superior surface area-to volume ratio, enormous porosity (can allow oxy-permeability) or reticular nano-porosity (can inhibit the microorganisms’adhesion), structural similitude to the skin extracellular matrix, and progressive electrospinning methodology, which promotes a prolonged drug release. The reason that we chose to review the formulation of electrospun nanofibers based on polysaccharides as dressings useful in wound healing was based on the ever-growing research in this field, research that highlighted many advantages of the nanofibrillary network, but also a marked versatility in terms of numerous active substances that can be incorporated for rapid and infection-free tissue regeneration. In this review, we have extensively discussed the recent advancements performed on electrospun nanofibers (eNFs) formulation methodology as wound dressings, and we focused as well on the entrapment of different active biomolecules that have been incorporated on polysaccharides-based nanofibers, highlighting those bioagents capable of improving the healing process. In addition, in vivo tests performed to support their increased efficacy were also listed, and the advantages of the polysaccharide nanofiber-based wound dressings compared to the traditional ones were emphasized.

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Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

Cited by 202 publications

References 49 publications

Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa

Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa

Silver Nanomaterials for Wound Dressing Applications

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

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