Wound Healing-Promoting and Melanogenesis-Inhibiting Activities of Angelica polymorpha Maxim. Flower Absolute In Vitro and Its Chemical Composition

Abstract

Angelica polymorpha Maxim. (APM) is used in traditional medicine to treat chronic gastritis, rheumatic pain, and duodenal bulbar ulcers. However, it is not known whether APM has epidermis-associated biological activities. Here, we investigated the effects of APM flower absolute (APMFAb) on responses associated with skin wound healing and whitening using epidermal cells. APMFAb was obtained by solvent extraction and its composition was analyzed by GC/MS. Water-soluble tetrazolium salt, 5-bromo-2′-deoxyuridine incorporation, Boyden chamber, sprouting, and enzyme-linked immunosorbent assays and immunoblotting were used to examine the effects of APMFAb on HaCaT keratinocytes and B16BL6 melanoma cells. APMFAb contained five compounds and induced keratinocyte migration, proliferation, and type IV collagen synthesis. APMFAb also induced the phosphorylations of ERK1/2, JNK, p38 mitogen-activated protein kinase, and AKT in keratinocytes. In addition, APMFAb decreased serum-induced B16BL6 cell proliferation and inhibited tyrosinase expression, melanin contents, and microphthalmia-associated transcription factor expression in α-melanocyte-stimulating hormone-stimulated B16BL6 cells. These findings demonstrate that APMFAb has beneficial effects on skin wound healing by promoting the proliferation, migration, and collagen synthesis of keratinocytes and on skin whitening by inhibiting melanin synthesis in melanoma cells. Therefore, we suggest that APMFAb has potential use as a wound healing and skin whitening agent.

Chemical Composition of Salix koreensis Anderss Flower Absolute and Its Skin Wound Healing Activities In Vitro

“…The skin healing effects of plants and plant extracts have been extensively investigated with a view toward developing agents that promote wound healing agents because of their minimal side effects and low development costs [ 28 , 29 ]. In the present study, we found that SKAFAb induced the proliferation and migration of HaCaT cells and increased HaCaT cell sprout growth in a collagen sprouting assay, which can simultaneously observe migration and proliferative ability [ 24 , 25 , 30 ]. These results confirm that SKAFAb can stimulate the proliferation and migration of keratinocytes.…”

“…Woodley et al reported that type I and type IV collagens increase keratinocyte migration [ 49 ]. Previous studies have shown many plant extracts enhance the syntheses and secretions of type I and/or IV collagens by HaCaT cells [ 25 , 30 , 50 ], which implies keratinocytes can synthesize and secrete collagen types I and IV. In the present study, SKAFAb increased collagen type I at concentrations of 150 μg/mL and 50 and IV syntheses 150 μg/mL in HaCaT cells.…”

“…SK-0003) was kept at the Herbarium of the College of Life and Health Science (Hoseo University, Korea). Absolute was extracted from SKA flowers by solvent extraction, as in previous reports [ 30 ]. In brief, SKA flowers (2.05 kg) were completely immersed in n-hexane (Samchun, Pyeongtaek, Korea) at room temperature (RT) for 1 h. Extracts were obtained, and the solvent was removed in a rotary evaporator (EYELA, Tokyo, Japan) at 25 °C under vacuum.…”

“…Components of SKAFAb were analyzed by GC/MS at the Korean Basic Science Institute (KSBI, Seoul, Korea) as in a previous report [ 30 ]. GC/MS analysis was conducted using a 7890BGC/7010QQQ MS instrument (Agilent, Palo Alto, CA, USA) and a DB5-MS capillary column (30 m × 0.25 mm, film thickness 0.25 μm).…”

GIF-2209, an Oxindole Derivative, Accelerates Melanogenesis and Melanosome Secretion via the Modification of Lysosomes in B16F10 Mouse Melanoma Cells

“…A number of small compounds that modulate melanogenesis have been reported and are categorized into several groups based on the mechanism of action, cellular signaling [ 4 , 5 ], transcription [ 6 ], translation [ 7 ], cellular trafficking [ 8 ], and degradation [ 9 ]. Post-translational modulators of melanogenic factors are often associated with intracellular trafficking and affect the maturation and fate of melanosomes and related organelles [ 10 ].…”