WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana

Arraño‐Salinas, Paulina; Domínguez-Figueroa, José; Herrera-Vásquez, Ariel; Zavala, Diego; Medina, Joaquı́n; Vicente‐Carbajosa, Jesús; Meneses, Claudio; Canessa, Paulo; Moreno, Adrián A.; Blanco‐Herrera, Francisca

doi:10.1016/j.plantsci.2018.09.019

Cited by 25 publications

(19 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultsmentioning

confidence: 85%

“…In contrast, the genes in Group 3 were dramatically repressed by aphid infestation in P746 ( Figure 4C). The expression patterns were confirmed by qPCR in Glyma14G34590 (GmRAP2), Glyma09G06980 (GmWRKY11), and Glyma03G00890 (GmMYB30), which were involved in stress response in plant [24][25][26] (Figure 4D). The expression patterns of TFs were investigated in all libraries, and k-mean clustering based on the expression patterns of the TF gene families resolved six clusters.…”

Section: Differentially Expressed Transcription Factors In Response Tmentioning

confidence: 76%

See 1 more Smart Citation

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

Yao

Yang

et al. 2020

IJMS

View full text Add to dashboard Cite

Soybean aphid (Aphis glycines Matsumura) is one of the major limiting factors in soybean production. The mechanism of aphid resistance in soybean remains enigmatic as little information is available about the different mechanisms of antibiosis and antixenosis. Here, we used genome-wide gene expression profiling of aphid susceptible, antibiotic, and antixenotic genotypes to investigate the underlying aphid–plant interaction mechanisms. The high expression correlation between infested and non-infested genotypes indicated that the response to aphid was controlled by a small subset of genes. Plant response to aphid infestation was faster in antibiotic genotype and the interaction in antixenotic genotype was moderation. The expression patterns of transcription factor genes in susceptible and antixenotic genotypes clustered together and were distant from those of antibiotic genotypes. Among them APETALA 2/ethylene response factors (AP2/ERF), v-myb avian myeloblastosis viral oncogene homolog (MYB), and the transcription factor contained conserved WRKYGQK domain (WRKY) were proposed to play dominant roles. The jasmonic acid-responsive pathway was dominant in aphid–soybean interaction, and salicylic acid pathway played an important role in antibiotic genotype. Callose deposition was more rapid and efficient in antibiotic genotype, while reactive oxygen species were not involved in the response to aphid attack in resistant genotypes. Our study helps to uncover important genes associated with aphid-attack response in soybean genotypes expressing antibiosis and antixenosis.

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Differentially Expressed Transcription Factors In Response Tmentioning

confidence: 76%

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

Yao

Yang

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…WRKY transcription factors modulate plant responses to biotic and abiotic stresses. In particular, WRKY7, -11, and -17 transcription factors are known to control AtBIP1/2 expression by suppressing the AtbZIP28 arm of the UPR during plant immunity 40 . The StBIP3 has CAMTA binding sites, which are lacking in the StBiP1/2…”

Section: Stbip Promoters Are Enriched With Developmental and Stress-rmentioning

confidence: 99%

“…hormonal regulation and stress response in plants. Some of the identified CREs include B3 (mediation of auxin and brassinosteroid dependent development transitions), BBM and RAP2.6 (in cell proliferation, cell morphogenesis, pathogen infection, salt stress, and osmotic stress responses) and RAP2.1 (dehydration response)[38][39][40]45,47,48,54 . Most importantly, we have identified various bZIP transcription factor binding CREs important for stress-mediated hormonal signaling in plants.…”

mentioning

confidence: 99%

Genome-wide identification and characterization ofSolanum tuberosum BiPgenes reveals the role of the promoter architecture in BiP gene diversity

Herath

Gayral

Adhikari

et al. 2020

Preprint

View full text Add to dashboard Cite

max)The endoplasmic reticulum (ER) immunoglobulin binding proteins (BiPs) are molecular chaperones involved in normal protein maturation and refolding malformed proteins through the unfolded protein response (UPR). Plants BiPs belong to a multi-gene family contributing to development, immunity, and responses to environmental stresses. This study identified three BiP homologues in the Solanum tuberosum (potato) genome using phylogenetic, amino acid sequence, 3-D protein modeling and gene structure analysis. These analyses revealed that StBiP1 and StBiP2 grouped with AtBiP2, whereas StBiP3 grouped with AtBiP3. While the protein sequences and folding structures are highly similar, these StBiPs are distinguishable by their expression patterns in different tissues and in response to environmental stressors such as treatment with heat, chemicals, or virus elicitors of UPR. Ab initio promoter analysis revealed that potato and Arabidopsis BiP1 and BiP2 promoters were highly enriched with cis regulatory elements (CREs) linked to developmental processes, whereas BiP3 promoters were enriched with stress-related CREs. The frequency and linear distribution of these CREs produced two phylogenetic branches that further resolve the groups identified through gene phylogeny and exon/intron phase analysis. These data reveal that the CRE architecture of BiP promoters potentially define their spatio -temporal expression patterns under developmental and stress related cues. IntroductionThe ER binding immunoglobulin protein (BiP), also known as the glucose receptor protein 78 (GRP78) is conserved across evolutionary kingdoms and is one of the best characterized molecular chaperones in the endoplasmic reticulum (ER). BiP guides the cotranslational translocation of nascent proteins into the ER, chaperones protein folding and maturation. BiP plays a key role in protein quality control by identifying and refolding misfolded proteins. BiP directs the post-translational translocation of proteins out of the ER. BiP contains an N-terminal nucleotide-binding domain (NBD) and a C-terminal substrate-binding domain (SBD). The NBD consists of two lobes surrounding the allosteric ATP-binding site, which modulates the substrate-binding activities. SBD consists of two subdomains, SBDβ and SBDα, which enable BiP to bind the hydrophobic surfaces of newly translated proteins to protect them from aggregation in an ATP-dependent manner. The SBDβ is a pocket with two primary loops that surround the nascent polypeptide and, the SBDα lid is covering this pocket 1 . All BiP proteins have C-terminal HDEL or KDEL signaling motif, which ensures their retention and function in the ER and an ATP-binding domain near the N-terminus 2 .Across eukaryotes, BiPs are among the most abundant chaperones in the ER and are directly engaged in regulating the unfolded protein response (UPR). Under normal conditions in mammalian cells, BiP binds to and inhibits the three ER stress sensors, protein kinase RNA-like ER kinase (PERK), activating transcription factor 6 (ATF6), and in...

show abstract

“…The current study aims to provide a functional characterization of CsWRKY7 , a member of the group IId WRKY family in tea plant. CsWRKY7 was a close homolog of Arabidopsis AtWRKY7 and AtWRKY15 , two well-characterized Group IId WRKY proteins with important roles in plant defense, leaf senescence, and abiotic stress responses [32,33,34]. When it is exposed to diverse stresses, CsWRKY7 is upregulated and localized to the nuclei in both tobacco leaves and Arabidopsis roots.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of CsWRKY7, a Subgroup IId WRKY Transcription Factor from Camellia sinensis, Linked to Development in Arabidopsis

Chen

et al. 2019

IJMS

View full text Add to dashboard Cite

WRKY transcription factors (TFs) containing one or two WRKY domains are a class of plant TFs that respond to diverse abiotic stresses and are associated with developmental processes. However, little has been known about the function of WRKY gene in tea plant. In this study, a subgroup IId WRKY gene CsWRKY7 was isolated from Camellia sinensis, which displayed amino acid sequence homology with Arabidopsis AtWRKY7 and AtWRKY15. Subcellular localization prediction indicated that CsWRKY7 localized to nucleus. Cis-acting elements detected in the promotor region of CsWRKY7 are mainly involved in plant response to environmental stress and growth. Consistently, expression analysis showed that CsWRKY7 transcripts responded to NaCl, mannitol, PEG, and diverse hormones treatments. Additionally, CsWRKY7 exhibited a higher accumulation both in old leaves and roots compared to bud. Seed germination and root growth assay indicated that overexpressed CsWRKY7 in transgenic Arabidopsis was not sensitive to NaCl, mannitol, PEG, and low concentration of ABA treatments. CsWRKY7 overexpressing Arabidopsis showed a late-flowering phenotype under normal conditions compared to wild type. Furthermore, gene expression analysis showed that the transcription levels of the flowering time integrator gene FLOWERING LOCUS T (FT) and the floral meristem identity genes APETALA1 (AP1) and LEAFY (LFY) were lower in WRKY7-OE than in the WT. Taken together, these findings indicate that CsWRKY7 TF may participate in plant growth. This study provides a potential strategy to breed late-blooming tea cultivar.

show abstract

WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana

Cited by 25 publications

References 52 publications

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

A Genome-Wide View of Transcriptional Responses during Aphis glycines Infestation in Soybean

Genome-wide identification and characterization ofSolanum tuberosum BiPgenes reveals the role of the promoter architecture in BiP gene diversity

Isolation and Characterization of CsWRKY7, a Subgroup IId WRKY Transcription Factor from Camellia sinensis, Linked to Development in Arabidopsis

Contact Info

Product

Resources

About