X-chromosome-wide profiling of MSL-1 distribution and dosage compensation inDrosophila

Abstract

In Drosophila, dosage compensation is achieved by a twofold up-regulation of the male X-linked genes and requires the association of the male-specific lethal complex (MSL) on the X chromosome. How the MSL complex is targeted to X-linked genes and whether its recruitment at a local level is necessary and sufficient to ensure dosage compensation remain poorly understood. Here we report the MSL-1-binding profile along the male X chromosome in embryos and male salivary glands isolated from third instar larvae using chromatin immunoprecipitation ( [Keywords: MSL; X chromosome; chromatin; dosage compensation; transcription] Supplemental material is available at http://www.genesdev.org.

De novotranscriptome sequencing to identify the sex-determination genes inHyriopsis schlegelii

“…However, the equal expression of X-linked genes between females and males is achieved through dosage compensation mechanisms. 36) Genetics studies have implicated three Msl genes, Msl1, Msl2, and Msl3 in dosage compensation; 37) these genes were also identified in the sexual glands of H. schlegelii. Realtime PCR analysis revealed that the expression levels of the three Msl genes were significantly higher in the sperm than in the ovary.…”

Imprinting of the Y Chromosome Influences Dosage Compensation inroX1 roX2 Drosophila melanogaster

“…In flies, dosage compensation is accomplished by the male-specific lethal (MSL) complex, composed of proteins and RNA. The MSL complex binds within the body of X-linked genes and alters chromatin to enhance transcription (Alekseyenko et al 2006;Gilfillan et al 2006;Legube et al 2006). The protein-coding components of the MSL complex were identified by the male-specific lethality of mutations in these genes.…”

High-resolution in situ hybridization analysis on the chromosomal interval 61C7-61C8 of Drosophila melanogaster reveals interbands as open chromatin domains

“…The sequences of the primers are available on request. Chromatin 537 immunoprecipitation was performed according to the protocol of Legube et al (2006). Chromatin 538 was prepared either from 10 7 S2 cells or 100 pairs of L3 salivary glands.…”