2010
DOI: 10.1107/s1744309110000461
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X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid

Abstract: PDB References: insulin, 3i3z; 3i40.Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged to the same space group, I2 1 3, with unit-cell parameters a = b = c = 77.365 Å , = = = 90.00. The reported space group and unit-cell parameters are almost identical to those of cubic insulin reported in the PDB. The results of X-ray studies confirmed that the crystals obtained were cubic insu… Show more

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Cited by 64 publications
(38 citation statements)
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References 17 publications
(16 reference statements)
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“…One can show that rmsd between this structure and PDB structure obtained at pH = 2.1 (PDB ID: 1GUJ) is 0.28 nm. The rmsd between PDB structure obtained at pH = 8.5 [50] (PDB ID: 3I3Z) and the most populated structure is 0.26 nm (Figure S6). Moreover, rmsd between 1GUJ and 3I3Z is about 0.32 nm implying the weak dependence of insulin structure on pH.…”
Section: Resultsmentioning
confidence: 99%
“…One can show that rmsd between this structure and PDB structure obtained at pH = 2.1 (PDB ID: 1GUJ) is 0.28 nm. The rmsd between PDB structure obtained at pH = 8.5 [50] (PDB ID: 3I3Z) and the most populated structure is 0.26 nm (Figure S6). Moreover, rmsd between 1GUJ and 3I3Z is about 0.32 nm implying the weak dependence of insulin structure on pH.…”
Section: Resultsmentioning
confidence: 99%
“…The starting coordinates of B20-30 were taken from the X-ray structure of human insulin (PDB code 3I3Z) [60]. The complex between IDE and the 1-18 portion of the B chain of insulin (PDB code 2WBY) [23] was used for the alignment of B20-30 prior to docking to IDE.…”
Section: Docking Simulationsmentioning
confidence: 99%
“…23-25 The three structures are virtually identical. Sequence alignment for the same set of proteins is shown in Figure 1b.…”
Section: Resultsmentioning
confidence: 94%