X-ray microanalysis of Rb+ entry into cricket Malpighian tubule cells<i>via</i>putative K+ channels

Marshall, A.T.; Clode, Peta L.

doi:10.1242/jeb.032177

Cited by 8 publications

(11 citation statements)

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“…The major route for K + entry into principal cells in the main segment of Malpighian tubules from the black field cricket, Teleogryllus oceanicus, is through Ba 2+ -sensitive channels (Marshall and Clode, 2009), and Ba 2+ reduces secretion by this tubule segment (Xu and Marshall, 1999b). However, passive ion entry through basolateral K + channels is not feasible in A. domesticus Malpighian tubules, because the electrochemical gradient is outwardly directed, and Ba…”

Section: Effect Of Ba 2+ On Tubule Secretionmentioning

confidence: 99%

“…Barium hyperpolarizes V b and inhibits secretion by Malpighian tubules from a number of species, which has been taken as evidence for K + channels contributing to transepithelial ion transport. In T. oceanicus, more than half of cellular K + is estimated to enter principal cells through Ba 2+ -sensitive channels (Marshall and Clode, 2009), and Ba 2+ inhibits fluid secretion (Xu and Marshall, 1999b). By contrast, the basolateral K + gradient of A. domesticus principal cells opposes passive ion entry, and K + channels therefore play no direct role in net transepithelial K + secretion, as evidenced by Ba 2+ having no effect on fluid secretion or K + transport, although it hyperpolarizes V b .…”

Section: Ion Conductance Pathways In the Basolateral Membranementioning

confidence: 99%

“…Bumetanide-sensitive Na + /K + /2Cl -cotransport has been demonstrated in the Malpighian tubules of R. prolixus and D. melanogaster (Ianowski et al, 2002;Ianowski and O'Donnell, 2004), and contributes to ion uptake in T. oceanicus (Xu and Marshall, 1999a;Marshall and Clode, 2009), A. aegypti (Hegarty et al, 1991;Scott et al, 2004), Manduca sexta (Audsley et al, 1993;Reagan, 1995), L. migratoria (Al-Fifi et al, 1998), Tenebrio molitor (Wiehart et al, 2003) and F. polyctena (Leyssens et al, 1994). A putative Na + /K + /2Cl -cotransporter has been cloned from the Malpighian tubules of M. sexta (Reagan, 1995), but mRNA encoding its homologue (CG31547) (Pullikuth et -cotransport is driven by the Na + electrochemical gradient ( bNa /F) across the basolateral membrane, which is maintained by active Na + extrusion into the lumen and bath.…”

Section: Ion Transport Across the Basolateral Membranementioning

confidence: 99%

“…Mechanisms for cation uptake across the basolateral membrane include an Na + /K + -ATPase (Torrie et al, 2004), cationchloride cotransporters (Na (Leyssens et al, 1994;Ianowski et al, 2002;Ianowski and O'Donnell, 2004), Ba 2+ -sensitive K + channels (Leyssens et al, 1993;Marshall and Clode, 2009), an Na + /H + exchanger (Pullikuth et al, 2006) and Na + -coupled organic anion transporters (Linton and O'Donnell, 2000). Depending largely on the contributions made by these transport process, primary urine can be Na + -rich, as in haematophagous insects, or K + -rich as in most other insects.…”

Section: Introductionmentioning

confidence: 99%

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Intracellular Na+, K+ and Cl− activities in Acheta domesticus Malpighian tubules and the response to a diuretic kinin neuropeptide

Coast

2012

Journal of Experimental Biology

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SUMMARY The mechanism of primary urine production and the activity of a diuretic kinin, Achdo-KII, were investigated in Malpighian tubules of Acheta domesticus by measuring intracellular Na+, K+ and Cl− activities, basolateral membrane voltage (Vb), fluid secretion and transepithelial ion transport. Calculated electrochemical gradients for K+ and Cl− across the basolateral membrane show they are actively transported into principal cells, and basolateral Ba2+-sensitive K+ channels do not contribute to net transepithelial K+ transport and fluid secretion. A basolateral Cl− conductance was revealed after the blockade of K+ channels with Ba2+, and a current carried by the passive outward movement of Cl− accounts for the hyperpolarization of Vb in response to Ba2+. Ion uptake via Na+/K+/2Cl− cotransport, driven by the inwardly directed Na+ electrochemical gradient, is thermodynamically feasible, and is consistent with the actions of bumetanide, which reduces fluid secretion and both Na+ and K+ transport. The Na+ gradient is maintained by its extrusion across the apical membrane and by a basolateral ouabain-sensitive Na+/K+-ATPase. Achdo-KII has no significant effect on the intracellular ion activities or Vb. Electrochemical gradients across the apical membrane were estimated from previously published values for the levels of Na+, K+ and Cl− in the secreted fluid. The electrochemical gradient for Cl− favours passive movement into the lumen, but falls towards zero after stimulation by Achdo-KII. This coincides with a twofold increase in Cl− transport, which is attributed to the opening of an apical Cl− conductance, which depolarises the apical membrane voltage.

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Section: Effect Of Ba 2+ On Tubule Secretionmentioning

confidence: 99%

Section: Ion Conductance Pathways In the Basolateral Membranementioning

confidence: 99%

Section: Ion Transport Across the Basolateral Membranementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intracellular Na+, K+ and Cl− activities in Acheta domesticus Malpighian tubules and the response to a diuretic kinin neuropeptide

Coast

2012

Journal of Experimental Biology

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“…На данный момент имеются десятки статей по: замещающему действию ионов Rb + на калиевые каналы (см., напр. : [88][89][90][91][92][93][94][95][96][97][98][99][100][101][102] [154,[156][157][158][159][160][161][162][163][164][165] и др. металлов.…”

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Correlational Patch-Clamp Spectrometry of the Ion Channels

Градов¹

2017

Preprint

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Membrane ion channels operate in accordance with the main principles of coordination chemistry. Coordination number is known to determine the ion selectivity of the sodium and potassium channels. there are known both ligand-dependent and ligand-controlled ion channels operating within the supramolecular coordination fixation principles. The channelforming ionophores form the structures which bind cations via coordination bonds leading to the conformational changes with the adjustment of the whole supramolecular architecture providing the ion channel selectivity. Such kind of non-covalent systems underlie the electrogenic membrane functions and the potential-controlled transmembrane ion transfer systems. They can be studied by means of the path-clamp method (i.e. the local membrane potential fixation), such as the «anion clamp», applied together with the analysis of the metal ion coordination geometry with the ion channels. However, such methods are not capable to register the ion channel conformational state in situ -during the ion coordination -with the molecular resolution of the ion channel structure. In this regard there is a need to develop dynamical methods capable of the simultaneous registration of the conformational and metallomic / elementomic coordination parameters and the electrophysiological response to the ion coordination. We propose to develop for this purpose a special kind of spectroscopic systems providing registration of the electrophysiological parameters and the single ion channel response by means of the local potential fixation techniques (patch-clamp / voltageclamp) with the advanced spectral processing and the subsequent data mining, with the simultaneous spectral detection of the ion channel state as a coordination and conformationally labile supramolecular structure, with the final data processing results presented not as the single spectra, but as the spectral correlogram.

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Distribution of Calcium and Phosphorus in Leaves of the Proteaceae

et al. 2014

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X-ray microanalysis of Rb+ entry into cricket Malpighian tubule cellsviaputative K+ channels

Abstract: SUMMARYElemental imaging by X-ray microanalysis of fully frozen-hydrated samples shows that when Malpighian tubules of the black field cricket (Teleogryllus oceanicus L.) are incubated in saline in which Rb + has been substituted for K +

Cited by 8 publications

References 34 publications

Intracellular Na+, K+ and Cl− activities in Acheta domesticus Malpighian tubules and the response to a diuretic kinin neuropeptide

Intracellular Na+, K+ and Cl− activities in Acheta domesticus Malpighian tubules and the response to a diuretic kinin neuropeptide

Correlational Patch-Clamp Spectrometry of the Ion Channels

Distribution of Calcium and Phosphorus in Leaves of the Proteaceae

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