In this paper, we demonstrated the comparison of digital-based CNT and analogue-based filament X-ray sources by taking the X-ray images of ACR mammo phantom for developing the intraoperative specimen X-ray system. The X-ray image of ACR mammo phantom taken at 25 kV 1 mA by filament shows overall the better image quality. However, when X-ray images of phantom were compared at 40 kV 1 mA by both sources, it showed that CNT X-ray source showed better image quality because of Aluminum window.