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Cervantes, Francisco J.; Duong-Dac, Tuan; Иванова, А. Е.; Roest, Kees De; Akkermans, A.D.L.; Lettinga, G.; Field, Jim A.

doi:10.1023/a:1021721929815

Cited by 25 publications

(4 citation statements)

References 17 publications

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“…Various anaerobic consortia from sludges and sediments were also shown to utilize AQDS as an electron acceptor (30). The two consortia utilized for the experiment were both cultivated with acetate and AQDS (25). The microbial consortia were incubated with acetate and a large amount of AC (20 or 40 g L -1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Reactor seed matter was anaerobic granular sludge from a full-scale UASB reactor treating alcohol distillery wastewater (Nedalco, Bergen op Zoom, The Netherlands). The inoculant of the biological AC reduction experiments was either crushed and diluted granular sludge from a lab-scale UASB reactor in which acetate oxidation was coupled to the reduction of AQDS or an AQDS/acetate enrichment culture derived from this sludge, which was predominated by a bacterium with 97% similarity to Geobacter sulfurreducens (25).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Activated Carbon as an Electron Acceptor and Redox Mediator during the Anaerobic Biotransformation of Azo Dyes

Zee

Bisschops

Lettinga

et al. 2002

Environ. Sci. Technol.

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275

162

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Activated carbon (AC) has a long history of applications in environmental technology as an adsorbent of pollutants for the purification of drinking waters and wastewaters. Here we describe novel role of AC as redox mediator in accelerating the reductive transformation of pollutants as well as a terminal electron acceptor in the biological oxidation of an organic substrate. This study explores the use of AC as an immobilized redox mediator for the reduction of a recalcitrant azo dye (hydrolyzed Reactive Red 2) in laboratory-scale anaerobic bioreactors, using volatile fatty acids as electron donor. The incorporation of AC in the sludge bed greatly improved dye removal and formation of aniline, a dye reduction product. These results indicate that AC acts as a redox mediator. In supporting batch experiments, bacteria were shown to oxidize acetate at the expense of reducing AC. Furthermore, AC greatly accelerated the chemical reduction of an azo dye by sulfide. The results taken as a whole clearly suggest that AC accepts electrons from the microbial oxidation of organic acids and transfers the electrons to azo dyes, accelerating their reduction. A possible role of quinone surface groups in the catalysis is discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Activated Carbon as an Electron Acceptor and Redox Mediator during the Anaerobic Biotransformation of Azo Dyes

Zee

Bisschops

Lettinga

et al. 2002

Environ. Sci. Technol.

Self Cite

275

162

View full text Add to dashboard Cite

show abstract

“…More than 85% of sequences clustered 100% with an uncultured Geobacter strain NS1 (AF404348). This strain was originally identified in a UASB survey reducing Fe(III) and quinones, 38 but has since been observed in acetate oxidising anodic biofilms. 39,40 The remaining sequences clustered evenly amongst largely Bacteroidetes, but also Firmicutes (all Clostridia) as well as Delta-and Betaproteobacteria.…”

Section: Monitoring Of Electrochemical Activity Of the Biofilmsmentioning

confidence: 99%

Non-invasive characterization of electrochemically active microbial biofilms using confocal Raman microscopy

Virdis

Harnisch

Batstone

et al. 2012

Energy Environ. Sci.

108

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Electrochemically active biofilms rely on microorganisms capable of extracellular electron transfer. Such biofilms are involved in the dissimilatory reduction of metal oxides in natural environments as well as electricity driving and driven processes at the electrodes of microbial bioelectrochemical systems. In this work we present the application of confocal Raman microscopy (CRM) as a non-invasive, label-free, and in vivo characterization method of acetate oxidizing anodic biofilms, grown from primary wastewater inoculum and dominated by Geobacter species (>85% of sequences analysed using pyrotag sequencing). Using the resonance Raman effect of the heme protein cytochrome c (Cyt c)-an ubiquitous component of extracellular electron transfer reactions-it was possible to collect characteristic spectral information of electrochemically active biofilms at pixel integration times of 0.2 s and an excitation wavelength of 532 nm. This allowed monitoring of biofilm development at different growth stages, without impacting its structural or metabolic activity. Furthermore, we demonstrate the possibility of non-invasive investigation of the spatial redox electrochemistry (up to a compositional level) of electrochemically active biofilms, as we observed significant changes in the vibrational properties of Cyt c resulting from shifts in the anodic potential between different redox conditions. Compared to conventional methods requiring destructive sample manipulation and fixation, the proposed approach based on CRM allows the non-invasive analysis of microbial aggregates with minimal sample preparation or prior knowledge of the sample

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“…Numerous bacteria, such as Geobacter species and Shewanella oneidensis , can utilize a wide range of terminal electron acceptors, including Fe(III), Co(III), U(VI), Tc(VII), fumarate, and humic acids. − Moreover, 6-methyladenine (m6A) and 4-methylcytosine (m4C) are the most prevalent types of DNA modifications in prokaryotes. − DNA methylation has various patterns based on culture conditions, , cell type, − and tissue type . However, the role of DNA methylation in the adaptation of microbes to diverse electron acceptors remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Methylome and Metabolome Analyses Reveal Adaptive Mechanisms in Geobacter sulfurreducens Grown on Different Terminal Electron Acceptors

Shi

2019

J. Proteome Res.

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The Geobacter species evolved respiratory versatility to utilize a wide range of terminal electron acceptors. To explore this adaptive mechanism, Fe(III) citrate, hydrous ferric oxide, and fumarate were selected as electron acceptors, and the methylome and metabolome of Geobacter sulfurreducens PCA grown on each electron acceptor were investigated via third-generation, single-molecule real-time DNA sequencing and gas chromatography/time-of-flight mass spectrometry-based metabolomics, respectively. Results showed that the patterns of 4-methylcytosine (m4C) and 6-methyladenine (m6A) modification, the concentrations of fatty acids (e.g., caprylic acid, capric acid, and squalene), and the activity of antioxidant enzymes (e.g., superoxide dismutase, catalase, and glutathione reductase) were all varied in different electron acceptor cultures. Moreover, genes (e.g., GSU0466 and GSU1467) with low expression levels generally had high methylation levels. These findings suggest that m4C and m6A modifications, fatty acids, and antioxidant enzymes all play a role in the adaptation of G. sulfurreducens to diverse electron acceptors, and DNA methylation may be involved in the adaptation mainly via gene expression regulation.

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Untitled

Cited by 25 publications

References 17 publications

Activated Carbon as an Electron Acceptor and Redox Mediator during the Anaerobic Biotransformation of Azo Dyes

Activated Carbon as an Electron Acceptor and Redox Mediator during the Anaerobic Biotransformation of Azo Dyes

Non-invasive characterization of electrochemically active microbial biofilms using confocal Raman microscopy

Methylome and Metabolome Analyses Reveal Adaptive Mechanisms in Geobacter sulfurreducens Grown on Different Terminal Electron Acceptors

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