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Shibli, Rida A.; Ajlouni, M. M.

doi:10.1023/a:1006468122819

Cited by 40 publications

(12 citation statements)

References 17 publications

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“…In treatments including BAP, shoot regeneration response changed with species and I. schachtii was found to be better than I. sari. The promotive effect of BAP alone or in combination with NAA-inducing callus and adventitious bulblet regeneration was previously reported in other Iris species (Kawase et al, 1995;Suzhen et al, 1999;Shibli and Ajlouni, 2000;Boltenkov and Zarembo, 2005;Boltenkov et al, 2007).…”

Section: Discussionsupporting

confidence: 54%

“…The MS medium with 0.5 mg/L TDZ plus 0.5 mg/L NAA and 1 mg/L TDZ plus 0.5 mg/L NAA produced the largest number of regenerated shoots per explant. Although TDZ has been proven to be effective for in vitro regeneration of various plants (Çöçü et al, 2004;Erişen et al, 2011;Nasırcılar et al, 2011), Shibli and Ajlouni (2000) reported that embryogenesis did not occur with TDZ treatment in Iris nigricans.…”

Section: Discussionmentioning

confidence: 99%

“…Iris species can be propagated from seeds, bulbs, or rhizomes with a low propagation speed like other geophytes (Jevremovic and Radojevic, 2002;Boltenkov et al, 2007). Therefore, in recent years, a number of studies have reported in vitro propagation of Iris species such as I. ensata (Yabuya et al, 1991;Boltenkov et al, 2007), I. germanica (Shimizu et al, 1997;Wang et al, 1999aWang et al, , 1999b, I. nigricans (Shibli and Ajlouni, 2000), I. pumila (Jevremovic and Radojevic, 2002), I. ensata, I. setosa, I. sanguinea (Boltenkov and Zarebno, 2005), I. hollandica (Fidalgo et al, 2005), I. atrofusca, I. petrana, I. vartanii (AlGabbiesh et al, 2006, and I. adriatica (Keresa et al, 2009) from a range of explants.…”

Section: Introductionmentioning

confidence: 99%

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Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Uzun¹,

Ilbaş²,

İpek³

et al. 2014

Turk J Agric For

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Uzun¹,

Ilbaş²,

İpek³

et al. 2014

Turk J Agric For

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“…Plantlet regeneration with a different cultivar of the same species, occurred both organogenically and embryogenically (Wang et al 1999a, b). Callus induced from leaf tissue of I. nigricans was used for protoplast and suspension cultures by Shibli and Ajlouni (2000). They found best embryogenesis occurred with 4.5 lM BA, and that zeatin and thidiazuron inhibited embryo formation.…”

Section: Irismentioning

confidence: 99%

Micropropagation of iridaceae—a review

Ascough

Erwin

Staden

2009

Plant Cell Tiss Organ Cult

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The Iridaceae contains many hundreds of attractive species that have been overlooked for horticultural development. The incredible variation in flower and leaf shape, size and colour suggest this little-tapped resource could offer great opportunities for developing new commercial ornamental products. Micropropagation has increasingly become a valuable tool assisting breeders to release new species and cultivars into the market more rapidly. Here we review the progress made in Iridaceae micropropagation genus by genus, and highlight the potential for future expansion in this field.

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“…(Gozu et al, 1993), I. nigricans Dinsm. (Shibli and Ajlouni, 2000), I. pumila L., I. reichenbachii Heuff. (Jevremović et al, 2006) and I. adriatica Trin.…”

Section: Sažetak Introductionmentioning

confidence: 99%

Plant regeneration and clonal fidelity assessment of subendemic species Iris illyrica Tomm. originated from Croatia

Mihovilović¹,

Barić²,

Bolarić³

et al. 2019

JCEA

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The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somatic embryogenesis of the subendemic Iris species Iris illyrica from Croatia and to select highly regenerative donor plants/genotypes. Leaf base segments were used as explants. Callogenesis and somatic embryogenesis were induced on MS media supplemented with: (1) 4.52 M 2,4-dichlorophenoxyacetic acid (2,4-D) + 4.83 M 1-naphthaleneacetic acid (NAA) + 0.46 M kinetin (Kin); (2) 4.52 M 2,4-D + 4.6 M Kin; (3) 13.4 M NAA + 2.3 M Kin. Transfer of embryogenic calli onto hormone-free medium enabled the development of mature somatic embryos. Frequency of callogenesis was influenced by the donor plant. Among 15 donor plants tested, 3 of them exhibited high regeneration capability and produced 87 regenerants. Seven morphological traits were observed in order to assess phenotypic variability of flowering regenerants. Regenerants with higher values of fall and standard width and length show potential for further breeding of new varieties of Illyrian iris. Flowering and non-flowering regenerants had the same ploidy level as donor plants. Also, the nuclear DNA content (2C = 12.936 ± 0.038 pg) of this species was estimated for the first time using flow cytometry.Ovo istraživanje provedeno je s ciljem procjene uspješnosti kalogeneze i regeneracije somatskom embriogenezom hrvatske subendemske vrste Iris illyrica, te odabira regenerabilnih donorskih biljaka/genotipova. Kao eksplantati korištene su baze listova. Kalogeneza i somatska embriogeneza inducirane su na MS mediju uz dodatak (1) 4,52 M 2,4-diklorofenoksioctene kiseline (2,4-D) + 4,83 M α-naftalenoctene kiseline (NAA) + 0,46 M kinetina (Kin); (2) 4,52 M 2,4-D + 4,6 M Kin i (3) 13,4 M NAA + 2,3 M Kin. Prijenosom embriogenih kalusa na medij bez dodatka regulatora rasta omogućen je razvoj zrelih somatskih embrija. Na uspješnost kalogeneze značajno su utjecale donorske biljke. Među 15 testiranih donorskih biljaka, tri su pokazale veliku sposobnost regeneracije i proizvele 87 regeneranata. U svrhu procjene fenotipske varijabilnosti cvatućih regeneranata, mjereno je sedam morfoloških karakteristika. Regeneranti s utvrđenom većom širinom i dužinom donjeg i gornjeg lista perigona u odnosu na matične biljke predstavljaju potencijalno vrijedan genetski materijal za selekciju novih kultivara Ilirske perunike za hortikulturnu primjenu. Cvatući i necvatući regeneranti imali su jednak stupanj ploidnosti kao i donorske biljke. Također, po prvi puta je procijenjen sadržaj jezgrine DNA (2C = 12,936 ± 0,038 pg) ove vrste upotrebom protočne citometrije.

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Untitled

Cited by 40 publications

References 17 publications

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Micropropagation of iridaceae—a review

Plant regeneration and clonal fidelity assessment of subendemic species Iris illyrica Tomm. originated from Croatia

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