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Гарбуз, Д. Г.; Molodtsov, Vasilii; Velikodvorskaia, V. V.; Евгеньев, М. Б.; Zatsepina, Olga G.

doi:10.1023/a:1016887812169

Cited by 14 publications

(2 citation statements)

References 27 publications

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“…Hsp protein expression is a balance between their protective role in various stressful conditions [ 62 , 63 ] and their high-energy demands, which may lead to reduced fitness under moderate and non-stressful conditions [ 30 , 64 , 65 , 66 , 67 ]. Although some results show that warm-adapted individuals tend to have a higher temperature threshold with increased Hsp70 protein and Hsp70 mRNA levels after heat stress compared to cold-adapted ones [ 68 , 69 , 70 , 71 , 72 ], several studies have reported the opposite trend [ 62 , 73 ]. In our experiment, all individuals reared in warm conditions tended to have higher Hsp70 expression than individuals reared in cold or optimal conditions, but Hsp70 expression after heat shock appeared to be population-specific, with a more pronounced rise in population from fluctuating climate factors in natural settings.…”

Section: Discussionmentioning

confidence: 99%

Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura

Erić

Veselinović

Patenković

et al. 2023

Life

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Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.

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Section: Discussionmentioning

confidence: 99%

Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura

Erić

Veselinović

Patenković

et al. 2023

Life

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“…According to previous reports, the localization and concentration of Hsp27 seems to be important in its biological function. Reference [5] supposed that HSP40 and lmwHSPs might play the main role in thermal adaptation of Drosophila. We observed increased HSP40 expression and number of isoforms of this family in all thermotolerant species and strains studied compared to thermosensitive ones.…”

Section: Introductionmentioning

confidence: 99%

Early-Phase Detection of Heat Shock Proteins (Hsp27 and Hsp40) in Extruded Earthworm Tissue after Dermal Exposure to Herbicides

Liu

Duan

Liu

et al. 2011

AMR

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Samples on a regular basis, and the use of SDSPAGE and Western Blotting methods was inspected to express situation in the induction of heat shock protein (both Hsp27 and Hsp40) of the body tissue of earthworms (Eisenia foetide) exposed in the soil by respectively adding Atrazine, Chlorotoluron; Acetochlor, Butachlor. According to the gel imaging system for gray-scale scanner, with the increase of the concentration of herbicides, the relative expression of both Hsps showed the phenomenon of induction in low concentration and suppression in high concentrations within the range of tested concentration. With the extension of the processing time, both Hsps were first induced suppression of the general trend and there was good exposure time – coercing effect relationship. The relative expression of HSP27 and HSP40 exposed by Atrazine, Chlorotoluron, Acetochlor and Butachlor were 2.21, 1.98, 2.06, 2.11 times and 2.32, 1.99, 2.15, 2.24 times of the control group. The sensitive ability of the organization of HSP27 and HSP40 to four herbicides was in the order of Atrazine > Butachlor > Acetochlor > Chlorotoluron. The relative expression of Hsp40 slightly was a little bit larger than Hsp27.

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