Xanthine oxidase inhibitors isolated from Piper nudibaccatum

Liu, Hongxin; He, Mengting; Tan, Haibo; Gu, Wei; Yang, Shi-Xian; Wang, Yue‐Hu; Li, Ling; Long, Chunlin

doi:10.1016/j.phytol.2015.03.005

Cited by 20 publications

(7 citation statements)

References 28 publications

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“…frutescens leaves were investigated using a bioassay-guided procedure to characterize active compounds for preventing and treating hyperuricemia. The enzyme activity has usually been measured spectrophotometrically by determing uric acid formation at 295 nm with xanthine as substrate [22,23]. Since direct spectrophotometry in UV region is prone to matrix interferences arising from the many organic compounds present in complex samples that also exhibit UV absorbance, uric acid formation was determined by an HPLC method in this study.…”

Section: Resultsmentioning

confidence: 99%

Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens

et al. 2015

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Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity. The crude extract showed expressive urate-lowering activity results. Solvent partitioning of the total extract followed by macroporous resin column chromatography of the n-butanol extract yielded four extracts and eluted parts. Among them, only the 70% ethanol eluted part of the n-butanol extract showed strong activity and therefore was subjected to separation and purification using various chromatographic techniques. Five compounds showing potent activity were identified by comparing their spectral data with literature values to be caffeic acid, vinyl caffeate, rosmarinic acid, methyl rosmarinate, and apigenin. These results indicate that pending further study, these compounds could be used as novel natural product agents for the treatment of hyperuricemia.

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Section: Resultsmentioning

confidence: 99%

Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens

et al. 2015

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“…However, they also cause side effects, such as allergic and hypersensitivity reactions, renal failure, skin rash, gastrointestinal distress, and enhancement of 6-mercaptopurine toxicity [6]. As a result of increasing consumer preference toward more natural and healthier products, scientific research has begun to focus on the screen of natural compounds as possible XO inhibitors [7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Chemical Evidence for Potent Xanthine Oxidase Inhibitory Activity of Ethyl Acetate Extract of Citrus aurantium L. Dried Immature Fruits

et al. 2016

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Xanthine oxidase is a key enzyme which can catalyze hypoxanthine and xanthine to uric acid causing hyperuricemia in humans. Xanthine oxidase inhibitory activities of 24 organic extracts of four species belonging to Citrus genus of the family Rutaceae were assayed in vitro. Since the ethyl acetate extract of C. aurantium dried immature fruits showed the highest xanthine oxidase inhibitory activity, chemical evidence for the potent inhibitory activity was clarified on the basis of structure identification of the active constituents. Five flavanones and two polymethoxyflavones were isolated and evaluated for inhibitory activity against xanthine oxidase in vitro. Of the compounds, hesperetin showed more potent inhibitory activity with an IC 50 value of 16.48 µM. For the first time, this study provides a rational basis for the use of C. aurantium dried immature fruits against hyperuricemia.

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“…Neotaiwanesol B ( 119 ) (IC 50 = 0.28 μM) was found to be the most potent XO inhibitor compared to allopurinol and hydroxychavicol (Fig. ) …”

Section: Xanthine Oxidasementioning

confidence: 99%

“…20). 193 In 2016, Wang and team found a potential -glucosidase and XO inhibitors from the plant Trifolium pratense L.…”

Section: Natural Nonpurine Analogsmentioning

confidence: 99%

Toward an Understanding of Structural Insights of Xanthine and Aldehyde Oxidases: An Overview of their Inhibitors and Role in Various Diseases

Kumar

Joshi

Kler

et al. 2017

Medicinal Research Reviews

101

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Almost all drug molecules become the substrates for oxidoreductase enzymes, get metabolized into more hydrophilic products and eliminated from the body. These metabolites sometime may be more potent, active, inactive, or toxic in nature compared to parent molecule. Xanthine oxidoreductase and aldehyde oxidase belong to molybdenum containing family and are well characterized for their structures and functions, in particular to their ability to oxidize/hydroxylate the xenobiotics. Their upregulated clinical levels causing oxidative stress are associated with pathways either directly involved in the progression of diseases, gout, or indirectly with the succession of other diseases such as diabetes, cancer, etc. Herein, we have put forth a comprehensive review on the xanthine and aldehyde oxidases pertaining to their structures, functions, pathophysiological role, and a comparative analysis of structural insights of xanthine and aldehyde oxidases' binding domains with endogenous ligands or inhibitors. Though both the enzymes are molybdenum containing and are likely to share some common pathways and interact with inhibitors in a similar manner but we have focused on structural prerequisites for inhibitor specificity to both the enzymes keeping in view of the existing X-ray structures. This review also provides futuristic implications in the design of inhibitors derived from inorganic complexes or small organic molecules considering the spatial features and structural insights of both the enzymes.

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Xanthine oxidase inhibitors isolated from Piper nudibaccatum

Cited by 20 publications

References 28 publications

Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens

Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens

Chemical Evidence for Potent Xanthine Oxidase Inhibitory Activity of Ethyl Acetate Extract of Citrus aurantium L. Dried Immature Fruits

Toward an Understanding of Structural Insights of Xanthine and Aldehyde Oxidases: An Overview of their Inhibitors and Role in Various Diseases

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