Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity. The crude extract showed expressive urate-lowering activity results. Solvent partitioning of the total extract followed by macroporous resin column chromatography of the n-butanol extract yielded four extracts and eluted parts. Among them, only the 70% ethanol eluted part of the n-butanol extract showed strong activity and therefore was subjected to separation and purification using various chromatographic techniques. Five compounds showing potent activity were identified by comparing their spectral data with literature values to be caffeic acid, vinyl caffeate, rosmarinic acid, methyl rosmarinate, and apigenin. These results indicate that pending further study, these compounds could be used as novel natural product agents for the treatment of hyperuricemia.

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The new ligand 2,2'-[2,3-naphthylenebis(oxy)]-bis(N,N-dibenzyl(acetamide) (L) and its Eu(III) and Tb(III) picrate complexes were synthesized. The complexes were characterized by elemental analysis, IR, TG-DSC and conductivity measurements. The luminescence properties of the two complexes were investigated. The result indicates that the triplet state energy level of the ligand matches better to the resonance level of Eu(III) than Tb(III) ion. Fluorescence intensity of the Eu(III) complex was reduced with the raising coordination ability of solvent. In addition, the DNA-binding properties of the two complexes have been investigated by electronic absorption, fluorescence and cyclic voltammetry. The results suggest that both complexes can bind to DNA via an intercalative binding mode and their binding affinity for DNA follows the order: Eu(III) complex > Tb(III) complex.