Xanthine Oxidase Inhibitory Activity of Extracts Prepared from Polygonaceae Species

Abstract

The xanthine oxidase (XO)-inhibitory activity of aqueous and organic extracts of 27 selected species belonging in five genera (Fallopia, Oxyria, Persicaria, Polygonum and Rumex) and P. viviparum demonstrated the highest XO-inhibitory activity (> 85% inhibition) at 400 µg/mL. The IC 50 values of the active extracts were also determined. On the basis of the results, these plants, and especially P. hydropiper and R. acetosella, are considered worthy of activity-guided phytochemical investigations.

Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract

“…The interest in possible health benefits of flavonoids has increased owing to their potent antioxidant and free radical scavenging activities observed in vitro 30 . According to numerous previous studies, flavonoids might be responsible for many biological activities of curly dock, such as antiviral, antibacterial, anticancer, antioxidant activities and they might be capable to activate antioxidant enzymes 2,3,6,7,9,[11][12][13][14] . However, most interest of this study was devoted to the antioxidant activity of flavonoids and investigated extract, due to their ability to reduce free radical formation and to scavenge free radicals 31 .…”

“…Conclusively, above mentioned extract can be considered as a potent carbohydrase inhibitor, anticancerous and antioxidant 12 . In addition, the extracts of curly dock root have demonstrated the high xanthine oxidase inhibitory activity 13 . The ethanol extracts of roots, leaves and fruits of curly dock have been screened for its cytotoxic activity in vitro and it has been found remarkable cytotoxic activities on leukemic 1301 -human T cell leukemia lymphoblast, and EOL-1 cell lines -human eosinophilic leukemia.…”

Three New Iridoid Glycosides from the Aerial Parts of Asperula involucrata

“…Xanthine oxidase isolated from bovine milk (lyophilized powder) and xanthine powder were purchased from Sigma–Aldrich . The production of uric acid by xanthine oxidase was measured at 290 nm for 3 min in 96‐well plate, using the plate reader FluoSTAR OPTIMA ( BMG LABTECH ) Fluorescence, in a total volume of 300 μl as described in the literature . Stock solutions were prepared as recommended: 50 m m potassium buffer, pH 7.5, 0.15 m m xanthine solution, pH 7.5 and XO enzyme 0.04 units/ml.…”

“…The production of uric acid by xanthine oxidase was measured at 290 nm for 3 min in 96-well plate, using the plate reader FluoSTAR OPTIMA (BMG LABTECH) Fluorescence, in a total volume of 300 ll as described in the literature. [21] Stock solutions were prepared as recommended: 50 mM potassium buffer, pH 7.5, 0.15 mM xanthine solution, pH 7.5 and XO enzyme 0.04 units/ml. The stock solutions of the extracts (12 g/ml) were prepared in DMSO solution.…”

Female versus male: Rumex thyrsiflorus Fingerh. under in vitro conditions. Does sex influence in vitro morphogenesis?

“…(thyrse sorrel) is a dioecious plant with polymorphic sex chromosomes. It belongs to the family Polygonaceae, which are rich sources of bioactive constituents that have a wide range of medicinal properties (Orbán-Gyapai et al 2015). The phytopreparations that can be isolated from R. thyrsiflorus exhibit, e.g., haemostatic, anti-inflammatory, antiscorbutic, and antiseptic activities (Litvinenko and Muzychkina 2008).…”