Xanthophyll esters are hydrolysed in the presence of recombinant human pancreatic lipase

Breithaupt, Dietmar E.; Alpmann, Alexander; Carrière, Frédéric

doi:10.1016/j.foodchem.2006.09.009

Cited by 14 publications

(3 citation statements)

References 23 publications

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“…The bioavailability of xanthophyll is highly dependent on the matrix due to its hydrophobicity of the long carbon skeletons, and thus dietary lipids are required to facilitate the dispersion of lutein, where similar matrix was adapted by pancreatic lipase [31]. Another study demonstrated xanthophyll ester hydrolysed by cholesteryl esterase, but not triacylglycerol lipase (pancreatic lipase), and thus ruled out the possibility of xanthophyll as a competitive substrate [32, 33]. To date, the mechanism and the inhibitory effect of xanthophyll on pancreatic lipase are still largely unknown, based on the literature findings that may lead to postulation that lutein may be a noncompetitive inhibitor by binding on the allosteric site of pancreatic lipase.…”

Section: Resultsmentioning

confidence: 99%

Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner

Ong

Mah

Lai

2016

Journal of Pharmaceutics

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Eleusine indica (Linnaeus) Gaertner is a traditional herb known to be depurative, febrifuge, and diuretic and has been reported with the highest inhibitory activity against porcine pancreatic lipase (PPL) among thirty two plants screened in an earlier study. This study aims to isolate and identify the active components that may possess high potential as an antiobesity agent. Of the screened solvent fractions of E. indica, hexane fraction showed the highest inhibitory activity of 27.01 ± 5.68% at 100 μg/mL. Bioactivity-guided isolation afforded three compounds from the hexane fraction of E. indica, namely, β-sitosterol, stigmasterol, and lutein. The structures of these compounds were elucidated using spectral techniques. Lutein showed an outstanding inhibitory activity against PPL (55.98 ± 1.04%), with activity 60% higher than that of the reference drug Orlistat. The other compounds isolated and identified were β-sitosterol (2.99 ± 0.80%) and stigmasterol (2.68 ± 0.38%). The enzyme kinetics of E. indica crude methanolic extract on PPL showed mixed inhibition mechanism.

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Section: Resultsmentioning

confidence: 99%

Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner

Ong

Mah

Lai

2016

Journal of Pharmaceutics

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“…Because of the interfacial activity of lipases, hydrolysis efficiency is directly influenced by the emulsification system. In a review of the literature, it was found that most studies on the hydrolysis of carotenoid esters (Liu and others 1998; Zorn and others 2003; Breithaupt and others 2007), especially of astaxanthin esters (Halldorsson and Haraldsson 2004; Grynbaum and others 2005) by lipases, used bile salt as emulsifier for substrates. While we reported Tween80 as an emulsifier mainly because a smaller particle size produced by Tween80 in emulsion could increase available surface area on the water‐oil interface and therefore accelerate the reaction speed, apart from economic reasons.…”

Section: Resultsmentioning

confidence: 99%

Astaxanthin Preparation by Lipase‐Catalyzed Hydrolysis of Its Esters from Haematococcus pluvialis Algal Extracts

Zhao¹,

Guan²,

Wang³

et al. 2011

Journal of Food Science

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Five of 8 fungal lipases screened were found to effectively hydrolyze astaxanthin esters from Haematococcus pluvialis algal cell extracts. Among these, an alkaline lipase from Penicillium cyclopium, expressed in Pichia pastoris, had the highest enzymolysis efficiency. Tween80 was shown to be an effective emulsifier in this lipase hydrolysis system for the 1st time. A series of experiments were performed to find optimal conditions for hydrolysis (pH, temperature, reaction time, lipase dosage). In the optimal reaction system, Tween80 and H. pluvialis extracts (mass ratio 1:1) were emulsified and added to the above lipase at a dosage of 4.6 U/μg (relative to total carotenoids), in phosphate buffer (0.1 M, pH 7.0), and incubated at 28 °C for 7 h, with agitation at 180 rpm. The free astaxanthin recovery ratio under these conditions was 63.2%.

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“…Whatever the aetiology, oral pancreatic enzyme supplements are widely used as the first-line approach to treat malabsorption secondary to exocrine pancreatic insufficiency (Breithaupt & al., 2007).…”

Section: Standard Approaches For the Treatment Of Fat Malabsorption Dmentioning

confidence: 99%

Emerging Approaches for the Treatment of Fat Malabsorption Due to Exocrine Pancreatic Insufficiency

Turki¹,

Kallel²

2012

New Advances in the Basic and Clinical Gastroenterology

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Xanthophyll esters are hydrolysed in the presence of recombinant human pancreatic lipase

Cited by 14 publications

References 23 publications

Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner

Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner

Astaxanthin Preparation by Lipase‐Catalyzed Hydrolysis of Its Esters from Haematococcus pluvialis Algal Extracts

Emerging Approaches for the Treatment of Fat Malabsorption Due to Exocrine Pancreatic Insufficiency

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