Xenoantisera to human DR antigens: Serological and immunochemical characterization

Abstract

Antibodies to DR antigens were detected using serological and immunochemical tests in sera from rabbits and goats immunized with cultured human B-lymphoid cells mixed with an anti-T-cell xenoantiserum or with partially purified DR antigens. After absorption with human red blood cells, cultured melanoma cells, and/or T-lymphoid cells, DR xenoantisera become specifically cytotoxic to B lymphocytes. Three out of nine sera tested with a panel of T-depleted peripheral lymphocytes and chronic lymphocytic leukemia cells showed correlation with DR alloantisera submitted to the Seventh International Histocompatibility Workshop. Although the correlation coefficients were lower than those obtained with DR alloantisera, the results obtained suggest that DR xenoantisera may recognize allotypic specificities.

DR (Ia-like) antigens on human melanoma cells. Serological detection and immunochemical characterization.

“…The resulting antisera were shown by serological and immunochemical tests to react specifically with the same antigenic structures detected by DR alloantisera. 2 Xenoantiserum 3634 which contained antibodies to the heavy chain of HLA-A and B antigens and to DR antigens (20) was obtained from a rabbit immunized with histocompatibility antigens solubilized from cultured WI-L2 lymphoid cells and partially purified by uhracentrifugal flotation in KBr (21). This xenoantiserum has been used successfully to purify DR antigens for amino acid sequence analysis (6).…”

Murine Monoclonal Antibodies against HLA Structures

“…Standard immunization of animals requires at least some purification of the human cell membrane and specific antibodies are obtained only after repeated and tedious absorptions. Inspite of some good results reported in the rabbit (Solheim et al 1978, Ferrone et al 1978, it was obvious that the lymphocyte "antigen" was a good candidate for attempting the production of monoclonal antibodies from hybridomas after xenogeneic immunization.…”