Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study

Abstract

Aim: In vitro evaluation of the influence of bovine xenogenic biomaterials on stem cells from human exfoliated deciduous teeth (SHEDs). The study was divided into three groups: 1) group C (control), containing only MSCs; 2) group BP, containing MSCs and Bonefill Porous®; 3) group BO, containing MSCs and Bio-Oss®. MSCs were derived from a deciduous tooth from a 7-year-old male donor. An aliquot of cells was subjected to immunophenotyping by flow cytometry. Cell viability (neutral red), cytotoxicity (MTT), and cell proliferation (crystal violet) assays were performed. All groups underwent morphological analysis by light microscopy (LM), and the biomaterial with superior performance was submitted to evaluation by scanning electron microscopy (SEM). Time points of 24, 48, and 72 h of culture were used. All results were evaluated with a significance level of 0.05. Results showed that both biomaterials maintained cell viability and cytotoxicity similar to the control. The BO group showed smaller cell proliferation compared to the other groups. In LM evaluation, the BP group showed more spread and adherent cells than the BO group. In SEM, cells of the BP group showed characteristics of more active cells than those of the control. Bovine xenogenic biomaterials positively influenced SHEDs, while the BP group seemed to present higher potential with SHEDs for future application within in vivo and/or clinical studies.

Levantamento de seio maxilar modificado utilizando a técnica do travesseiro. Relato de caso clínico

“…Os biomateriais xenógenos possuem propriedades ostecondutoras, sendo produzidos por meio de processos de purificação em vários estágios, sendo que o biomaterial utilizado no estudo é vastamente relato na literatura como capaz de se integrar ao osso, tendo baixa taxa de reabsorção e altas taxas de sucesso (Manfro et al, 2014;Fadel et al, 2018;Stroparo et al, 2021). Quanto à utilização de membranas de colágeno, elas já são amplamente utilizadas na regeneração óssea guiada, no qual o uso de membranas protege o biomaterial enxertado, ocasionando preservações de enxertos e de alvéolos após extrações (Pang et al, 2014;Fadel et al, 2018;Troiano et al, 2018;Piovezana et al, 2020).…”

“…Devido à necessidade da reabilitação com implantes dentários, a cirurgia de levantamento do seio maxilar é descrita como uma técnica segura, nas quais podem ser utilizados biomateriais autógenos, alógenos, xenógenos e sintéticos (Manfro et al, 2014;Stroparo et al, 2021). Contudo, mesmo com o biomaterial autógeno sendo considerado o atual padrão ouro, os biomateriais xenógenos, que geralmente tem origem bovina ou eqüina, apresentam boa biocompatibilidade e uma morfologia semelhante a do osso humano, tendo várias vantagens, como baixa taxa de absorção, menos morbidade ao paciente, uma disponibilidade ilimitada, as quais são associadas a altas taxas de sucesso, pois esse biomaterial serve de arcabouço para formação de osso maduro (Riben & Thor 2012;Manfro et al, 2014;Stroparo et al, 2021).…”

Basic responses of mesenchymal stem cells exposed to bovine biomaterial and platelet rich fibrin

“…The Research Ethics Committee of Universidade Positivo approved the study under number 3.6665.172/2019. The methodology herein presented followed de Oliveira et al (2020), Lisboa et al (2021) and Stroparo et al (2021). Research, Society and Development, v. 10, n. 11, e46101119134, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i11.19134 4…”

“…Mesenchymal stem cells were characterized by Dominici protocols (2006). This methodology was describeb by Lisboa et al (2021) and Stroparo et al (2021).…”