Xenonuclear transplantation of buffalo (Bubalus bubalis) fetal and adult somatic cell nuclei into bovine (Bos indicus) oocyte cytoplasm and their subsequent development

Saikhun, Jumnian; Pavasuthipaisit, K.; Jaruansuwan, Mayurachat; Kitiyanant, Yindee

doi:10.1016/s0093-691x(02)00667-2

Cited by 45 publications

(39 citation statements)

References 23 publications

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“…Moreover, some embryos were able to form compacting morula on day 6 with large active polygonal nucleoli. Donor cell culture effects on the preimplantation development of SCNT embryos might be even more profound in the case of iSCNT embryos and explains the difference in developmental competence of buffalo-bovine iSCNT embryos in our experiments and other laboratories (Kitiyanant et al 2001, Saikhun et al 2002, Lu et al 2005. Cattle-water buffalo hybrid IVF BLs serve as another confirmation of nuclear-cytoplasm compatibility between these species (Kochhar et al 2002).…”

Section: Discussionsupporting

confidence: 54%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has been achieved in closely related species. The analyses of embryonic gene activity in iSCNT embryos of different species combinations have revealed the existence of significant aberrations in expression of housekeeping genes and genes dependent on the major embryonic genome activation (EGA). However, there are many studies with successful blastocyst (BL) development of iSCNT embryos derived from donor cells and oocytes of animal species with distant taxonomical relations (inter-family/inter-class) that should indicate proper EGA at least in terms of RNA polymerase I activation, nucleoli formation, and activation of genes engaged in morula and BL formation. We investigated the ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species. In iSCNT embryos, nucleoli precursor bodies originate from the oocyte, while most proteins engaged in the formation of mature nucleoli should be transcribed from genes de novo in the donor nucleus at the time of EGA. Thus, the success of nucleoli formation depends on species compatibility of many components of this complex process. We demonstrate that the time and cell stage of nucleoli formation are under the control of recipient ooplasm. Oocytes of the studied species possess different abilities to support nucleoli formation. Formation of nucleoli, which is a complex but small part of the whole process of EGA, is essential but not absolutely sufficient for the development of iSCNT embryos to the morula and BL stages.

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Section: Discussionsupporting

confidence: 54%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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“…Buffalo fetal fibroblasts were obtained from a 40-day-old fetus and were cultured as described previously [19]. The head and internal organs were removed and the remnant tissues were cut into small pieces.…”

Section: Donor Cell Preparationmentioning

confidence: 99%

“…Nuclear transfer was performed by the method previously described [19,21]. Immediately prior to NT, a single cell suspension was prepared from donor cells by standard trypsinization using 0.25% trypsin-EDTA.…”

Section: Nuclear Transfer Fusion and Activationmentioning

confidence: 99%

Development of swamp buffalo (Bubalus bubalis) embryos after parthenogenetic activation and nuclear transfer using serum fed or starved fetal fibroblasts

et al. 2004

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-The knowledge of oocyte activation and somatic cell nuclear transfer in the swamp buffalo (Bubalus bubalis) is extremely rare. The objectives of this study were the following: (1) to investigate the various activation treatments on the parthenogenetic development of buffalo oocytes, (2) to examine the events of nuclear remodeling and in the in vitro development of cloned buffalo embryos reconstructed with serum fed or starved fetal fibroblats, and (3) to investigate the in vivo development of cloned embryos derived from serum fed or starved cells after transfer into the recipients. The rates of cleavage and blastocyst development were found to be significantly higher (P < 0.05) when the oocytes were activated by the combination treatment of calcium ionophore (A23187) or ethanol followed by 6-DMAP than those activated by electrical pulses and 6-DMAP or other single treatments. Flow cytometric analysis revealed that the percentage in the G0/G1 phase in serum starved cells was significantly (P < 0.05) higher than that in serum fed cells (88.8 ± 6.2 vs. 68.2 ± 2.6). At 1 h post fusion (hpf), most of the transferred nuclei (71%) from serum fed cells did not change in size, and the nuclear envelope remained intact, whereas 29% underwent NEBD and PCC. When serum starved cells were used, 83% of the transferred nuclei underwent NEBD and PCC whereas 17% remained intact. The nuclear swelling and pronucleus (PN) formation were observed at 2-4 and 12 h post activation (hpa), respectively. The remodeled nuclei underwent mitotic division and developed to the 2-cell stage within 18-24 hpa. Fifty-five percent of oocytes reconstructed with serum fed cells were 2PN and 45% were 1PN, whereas 79% of the embryos reconstructed from starved cells were 1PN and 21% were 2PN. The percentage of blastocyst development of the embryos derived from starved cells was higher than that from the serum fed cells (35% vs. 21%, P < 0.05). Pregnancy was detected after the transfer of cloned blastocysts into the recipients but no recipients supported the development to term. The results of this work can be used to establish effective activation protocols for buffalo oocytes which can be used during nuclear transfer experiments.buffalo / oocyte activation / nuclear transfer / nuclear remodeling / somatic cell * Corresponding author: scykt@mahidol.ac.th 66 J. Saikhun et al.

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“…Compatible species are those that are highly phylogenetically related to those above listed and include for 1. gaur (Bos gaurus/B. frontalis; Lanza et al, 2000a; Figure 1) and swamp buffalo (Bubalus bubalis; Nguyen et al, 2000;Kitiyanant et al, 2001;Saikhun et al, 2002); 2. argali (Ovis ammon; White et al, 1999), and mouflon (Ovis orientalis musimon; Loi et al, 2001), and 3. African wild cat (Felis silvestris libica; Gomez et al, 2003Gomez et al, , 2004b, respectively.…”

Section: Consequences Resulting From the Transmission Of Two Mitochonmentioning

confidence: 99%

The effect of mitochondrial genome on architectural remodeling and epigenetic reprogramming of donor cell nuclei in mammalian nuclear transfer-derived embryos

Samiec¹

2005

J. Anim. Feed Sci.

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There are some species-specific epigenetic factors present in the oocyte cytoplasm that may contribute to nucleo-cytoplasmic incompatibilities either immediately after nuclear transfer (NT) or at later stages of development. These potential incompatibilities will affect, to some degree, the ultimate utility of NT technology. It has been demonstrated that maternally inherited mitochondrial DNA molecules (mtDNAs) accumulated in the mitochondrial reservoirs of recipient-oocyte cytosol play an important role in nuclear-ooplasmic asynchrony. This asynchrony involves the incompatibility in epigenetic modifications of somatic genome supporting the developmental program of reconstituted cybrids. It also involves incompatibility in molecular mechanisms controlling the karyokinesis and cytokinesis restriction points responsible for coordinated pseudomeiotic to mitotic cycle transition following activation of the reconstituted oocyte. Moreover, the presence of oocyte-derived mitochondrial genetic apparatus influences clonal embryo implantation. For that reason, the deleterious effect on preimplantation development of NT embryos of heterogeneous mtDNA sources arising from possible mitochondrial heteroplasmy in the reconstructed nuclear-cytoplasmic hybrids, should not be discounted. That is why, the production of nuclear transfer embryos, foetuses and offspring with precisely defined constellations of nuclear and/or mitochondrial genome can be valuable for experimentally dissecting the effects of nuclear and cytoplasmic genetic/epigenetic components as well as the intrauterine environment on embryonic, foetal, and postnatal development of cloned individuals.

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Xenonuclear transplantation of buffalo (Bubalus bubalis) fetal and adult somatic cell nuclei into bovine (Bos indicus) oocyte cytoplasm and their subsequent development

Cited by 45 publications

References 23 publications

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Development of swamp buffalo (Bubalus bubalis) embryos after parthenogenetic activation and nuclear transfer using serum fed or starved fetal fibroblasts

The effect of mitochondrial genome on architectural remodeling and epigenetic reprogramming of donor cell nuclei in mammalian nuclear transfer-derived embryos

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