XFCT-MRI hybrid multimodal contrast agents for complementary imaging

Saladino, Giovanni Marco; Vogt, Carmen; Brodin, Bertha; Shaker, Kian; Kilic, Nuzhet I.; Andersson, Ken G.; Arsenian-Henriksson, Marie; Toprak, Muhammet S.; Hertz, Hans M.

doi:10.1039/d2nr05829d

Cited by 10 publications

(11 citation statements)

References 67 publications

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“…S5, † the electron diffraction (SAED) patterns of the four SP-NC types are presented: they all exhibit the same peculiar pattern, which is associated with magnetite (or maghemite), demonstrating the absence of the other iron oxide crystal phase (hematite), which is not superparamagnetic. [37][38][39] The organic content of the SP-NCs was estimated via TGA; the resultant data are presented in Fig. S6.…”

Section: Papermentioning

confidence: 99%

Magnetoresponsive fluorescent core–shell nanoclusters for biomedical applications

et al. 2023

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Section: Papermentioning

confidence: 99%

Magnetoresponsive fluorescent core–shell nanoclusters for biomedical applications

et al. 2023

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“…In recent years, XRF was demonstrated to be employable for molecular imaging in vivo, using metal liquid jet as the X-ray source (24 keV) and contrast agents containing elements matching their absorption edge (core electron excitation) with the source energy, e.g., Rh, among others. ,, In Figure a, the X-ray spectrum collected by the XRF detectors when scanning a vial was presented. In the vial containing the resin with dispersed Rh NPs, it was possible to detect the Rh Kα radiation (20.2 keV) originating from Rh NPs, contrarily to control.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, capitalizing on being a contrast agent for X-ray fluorescence (XRF) imaging, we demonstrated the presence and uniform distribution of Rh NPs in the printed structures, highlighting the prospective use in diverse applications within the field of implantable medical devices. 20 − 22 …”

Section: Introductionmentioning

confidence: 99%

Two-Photon Polymerization Printing with High Metal Nanoparticle Loading

Kilic,

Saladino,

Johansson

et al. 2023

ACS Appl. Mater. Interfaces

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Two-photon polymerization (2PP) is an efficient technique to achieve high-resolution, three-dimensional (3D)-printed complex structures. However, it is restricted to photocurable monomer combinations, thus presenting constraints when aiming at attaining functionally active resist formulations and structures. In this context, metal nanoparticle (NP) integration as an additive can enable functionality and pave the way to more dedicated applications. Challenges lay on the maximum NP concentrations that can be incorporated into photocurable resist formulations due to the lasertriggered interactions, which primarily originate from laser scattering and absorption, as well as the limited dispersibility threshold. In this study, we propose an approach to address these two constraints by integrating metallic Rh NPs formed ex situ, purposely designed for this scope. The absence of surface plasmon resonance (SPR) within the visible and near-infrared spectra, coupled with the limited absorption value measured at the laser operating wavelength (780 nm), significantly limits the laser-induced interactions. Moreover, the dispersibility threshold is increased by engineering the NP surface to be compatible with the photocurable resin, permitting us to achieve concentrations of up to 2 wt %, which, to our knowledge, is significantly higher than the previously reported limit (or threshold) for embedded metal NPs. Another distinctive advantage of employing Rh NPs is their role as promising contrast agents for X-ray fluorescence (XRF) bioimaging. We demonstrated the presence of Rh NPs within the whole 2PP-printed structure and emphasized the potential use of NP-loaded 3D-printed nanostructures for medical devices.

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“…Despite not observing any behavioral changes or pathological signs in mice, previous histology studies of extracted organs denoted the presence of local thrombi in mice injected with MoO 2 NPs ( 27 ), probably related to the presence of NP accumulations in lungs, which were successfully detected by XRF molecular imaging. The possibility to track contrast agents in the lungs with XRF imaging constitutes a major advantage compared to MRI, a standard clinical imaging technique that has though limited applications for lung studies, due to susceptibility artifacts originating at the air-tissue interfaces ( 41 , 42 ). In this respect, the sensitivity for MoO 2 NPs was previously estimated to be on the order of 10 −2 mg/ml ( 27 ), and multimodal contrast agents evidenced a similar sensitivity for XFCT and contrast-enhanced MRI ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

“…The possibility to track contrast agents in the lungs with XRF imaging constitutes a major advantage compared to MRI, a standard clinical imaging technique that has though limited applications for lung studies, due to susceptibility artifacts originating at the air-tissue interfaces ( 41 , 42 ). In this respect, the sensitivity for MoO 2 NPs was previously estimated to be on the order of 10 −2 mg/ml ( 27 ), and multimodal contrast agents evidenced a similar sensitivity for XFCT and contrast-enhanced MRI ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Iterative nanoparticle bioengineering enabled by x-ray fluorescence imaging

Saladino,

Brodin,

Kakadiya

et al. 2024

Sci. Adv.

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Nanoparticles (NPs) are currently developed for drug delivery and molecular imaging. However, they often get intercepted before reaching their target, leading to low targeting efficacy and signal-to-noise ratio. They tend to accumulate in organs like lungs, liver, kidneys, and spleen. The remedy is to iteratively engineer NP surface properties and administration strategies, presently a time-consuming process that includes organ dissection at different time points. To improve this, we propose a rapid iterative approach using whole-animal x-ray fluorescence (XRF) imaging to systematically evaluate NP distribution in vivo. We applied this method to molybdenum-based NPs and clodronate liposomes for tumor targeting with transient macrophage depletion, leading to reduced accumulations in lungs and liver and eventual tumor detection. XRF computed tomography (XFCT) provided 3D insight into NP distribution within the tumor. We validated the results using a multiscale imaging approach with dye-doped NPs and gene expression analysis for nanotoxicological profiling. XRF imaging holds potential for advancing therapeutics and diagnostics in preclinical pharmacokinetic studies.

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XFCT-MRI hybrid multimodal contrast agents for complementary imaging

Abstract: Multimodal contrast agents in biomedical imaging enable the collection of more comprehensive diagnostic information. In the present work, we design hybrid ruthenium-decorated superparamagnetic iron oxide nanoparticles (NPs) as the contrast...

Cited by 10 publications

References 67 publications

Magnetoresponsive fluorescent core–shell nanoclusters for biomedical applications

Magnetoresponsive fluorescent core–shell nanoclusters for biomedical applications

Two-Photon Polymerization Printing with High Metal Nanoparticle Loading

Iterative nanoparticle bioengineering enabled by x-ray fluorescence imaging

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