xMAP Technology: Applications in Detection of Pathogens

Reslová, Nikol; Michna, Veronika; Kašný, Martin; Mikel, Pavel; Králík, Petr

doi:10.3389/fmicb.2017.00055

Cited by 96 publications

(74 citation statements)

References 96 publications

(131 reference statements)

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“…The Luminex xMAP technology is useful for many different applications. One review describes the use of this technology for the multiplex detection of viral, bacterial, parasitic, and fungal agents using the microsphere-based multiplex nucleic acid assay (MBMNA) and the microsphere-based multiplex immunoassay (MBMI) [16]. MBMIs are typically biochemical tests that allow the detection or measurement of the concentration of a protein in a solution via an antibody or immunoglobulin [17].…”

Section: Discussionmentioning

confidence: 99%

Development of a sensitive and specific xMAP assay for detection of antibodies against infectious laryngotracheitis and bronchitis viruses

Wang

Feng

Guan

et al. 2018

Virol J

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BackgroundA serological method to simultaneously detect antibodies against infectious laryngotracheitis virus (ILTV) and infectious bronchitis virus (IBV) is imperative for the differential diagnosis and evaluation of antibodies titers after vaccination.MethodThe microspheres coated with purified recombinant glycoprotein D (gD) of ILTV or nucleocapsid (N) protein of IBV were incubated with serum samples. The simultaneous quantification of ILTV and IBV antibodies were achieved through the interrogation of microspheres by Luminex 200 detection system. .ResultsThis xMAP detection demonstrated no nonspecific reactions with avian influenza virus (AIV), avian leukosis virus (ALV), newcastle disease virus (NDV), and Marek’s disease virus (MDV). The results also demonstrated that the xMAP assay was four times more sensitive than the enzyme-linked immunosorbent assay (ELISA) for ILTV detection and two times more sensitive for IBV detection. A total of 90 chicken serum samples from a chicken farm were tested by xMAP and ELISA assays. The results showed that the coincidence rates were 84.44 and 100% for ILTV and IBV detection, respectively.ConclusionThis study exhibited an opportunity for the differential diagnosis through simultaneous detection of multiplex antibodies in serum and can be used for the multiplex antibodies evaluation after vaccination.

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Section: Discussionmentioning

confidence: 99%

Development of a sensitive and specific xMAP assay for detection of antibodies against infectious laryngotracheitis and bronchitis viruses

Wang

Feng

Guan

et al. 2018

Virol J

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“…The use of magnetic particles enables the high washing efficiency of the target separation step to rapidly remove the unwanted components, resulting in the enhancement of the limit of detection. Based on those advantages, widely used fluorescence-based technologies for multiple/highly sensitive detections using the magnetic beads, such as Luminex xMAP [58][59][60][61], magnetic modulation biosensing (MMB) [62], Quanterix Simoa [63,64] and magnetically-modulated optical nanoprobes (MagMOONs) [65,66], have emerged. A recent study by Wang et al pointed out a microfluidic biosensing device modification platform for Salmonella typhimurium measurement using fluorescent labeling and video processing on smartphones.…”

Section: Fluorescent Biosensing Devicesmentioning

confidence: 99%

Magnetic Particles: Their Applications from Sample Preparations to Biosensing Platforms

et al. 2020

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The growing interest in magnetic materials as a universal tool has been shown by an increasing number of scientific publications regarding magnetic materials and its various applications. Substantial progress has been recently made on the synthesis of magnetic iron oxide particles in terms of size, chemical composition, and surface chemistry. In addition, surface layers of polymers, silica, biomolecules, etc., on magnetic particles, can be modified to obtain affinity to target molecules. The developed magnetic iron oxide particles have been significantly utilized for diagnostic applications, such as sample preparations and biosensing platforms, leading to the selectivity and sensitivity against target molecules and the ease of use in the sensing systems. For the process of sample preparations, the magnetic particles do assist in target isolation from biological environments, having non-specific molecules and undesired molecules. Moreover, the magnetic particles can be easily applied for various methods of biosensing devices, such as optical, electrochemical, and magnetic phenomena-based methods, and also any methods combined with microfluidic systems. Here we review the utilization of magnetic materials in the isolation/preconcentration of various molecules and cells, and their use in various techniques for diagnostic biosensors that may greatly contribute to future innovation in point-of-care and high-throughput automation systems.

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“…Enzyme linked immunosorbent assay (ELISA)-based systems are generally widely used for diagnosis of single microbial infection. Recent advancements using the Luminex xMAP technology offer multiplex capability (Reslova et al, 2017). The MagPix assay system based on ELISA principles is centered on paramagnetic microsphere technology, which can transition ELISAs to a more sensitive and consistent system with the added capability of multiplexing.…”

Section: Immunological Assaysmentioning

confidence: 99%