XPS and NMR analyze the combined forms of Pb in Cladophora rupestris subcells and its detoxification

Chen, Qiu-yu; Liu, Yang; Liu, Lei; Li, Xuan-xuan; Li, Haidong; Zhang, Qian; Cao, De-ju

doi:10.1007/s11356-022-19880-x

Cited by 12 publications

(11 citation statements)

References 61 publications

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“…Then, 2.0 g samples were grown in 1000 mL BBM and contained various concentrations of Pb(NO 3 ) 2 . Pb concentrations were set as 0, 1, and 5.0 mg/L, while the Pb stress time was set as 3 and 5 days [ 7 , 8 , 9 ]. The samples were numbered as Pb1_3 and Pb1_5 for the 1.0 mg/L treatment group on the third and fifth day, and Pb5_3 and Pb5_5 for the 5 mg/L treatment group on the third and fifth day, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Cladophora rupestris has a strong ability to absorb and tolerate Pb 2+ stress and alter the distribution of Pb at both the tissue and sub-cellular levels to reduce toxicity [ 4 , 5 ]. However, the tolerance and detoxification mechanisms of hyperaccumulate were complex [ 6 , 7 , 8 , 9 ]. Pb 2+ enter into the Chladophora cells by forming chelate with GSH/MT and –OH functional groups [ 7 ], and HMs transport plays an important role in the detoxification of plants by sequestering toxic or excess HMs into the vacuoles [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, the tolerance and detoxification mechanisms of hyperaccumulate were complex [ 6 , 7 , 8 , 9 ]. Pb 2+ enter into the Chladophora cells by forming chelate with GSH/MT and –OH functional groups [ 7 ], and HMs transport plays an important role in the detoxification of plants by sequestering toxic or excess HMs into the vacuoles [ 10 ]. The defense mechanism of Cassia alata is based on Cd accumulation in roots, coupled with an increase in GSH [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Liu

Zhang²,

Liu³

et al. 2022

IJERPH

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This study aimed to analyze the transcriptome of C. rupestris under Pb2+ stress by using high-throughput sequencing technology, observe the changes of gene expression and metabolic pathway after three and five days under 1.0 and 5.0 mg/L of Pb2+ treatment, and analyze the differentially expressed genes (DEGs) and related functional genes after Pb2+ treatment. Metabolic pathways were revealed through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results show that DEGs increased significantly with the increase of Pb2+ concentration and stress time. A total of 32 genes were closely related to Pb2+ stress response. GO analysis identified two major transporter proteins, namely, ATP-binding transport protein-related (ABC transporters) and zinc finger CCHC domain containing protein (Zfp) in C. rupestris. Pthr19248, pthr19211, Zfp pthr23002, Zfp p48znf pthr12681, Zfp 294 pthr12389, and Zfp pthr23067 played important roles against Pb2+ toxicity and its absorption in C. rupestris. KEGG pathway analysis suggested that ABCA1, ATM, and ABCD3 were closely related to Pb2+ absorption. Pb2+ stress was mainly involved in metallothionein (MT), plant hormone signal transduction, ABC transporters, and glutathione (GSH) metabolism.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Liu

Zhang²,

Liu³

et al. 2022

IJERPH

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“…Heavy metals (HMs) are persistent environmental contaminants that adversely affect the growth and survival of plants [1]. Lead (Pb) is one of the most hazardous pollutants, and it can seriously threaten human health [2]. Phytochelatins (PCs) exist widely in plants and are closely related to plant resistance to HMs.…”

Section: Introductionmentioning

confidence: 99%

“…PCs such as glutathione (GSH) and nonprotein thiols (NPTs) exhibit _________________________ 1 Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China, corresponding author D.-J. Cao, email address: cdj@ahau.edu.cn 2 School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China.…”

Section: Introductionmentioning

confidence: 99%

Phytochelatins of Cladophora rupestris in Pb2+ absorption and its detoxification

Lu-Sheng Zhang,

Liu Yang,

Lingsheng Li

et al. 2023

Environ. Protect. Eng.

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Phytochelatins (PCs) exist widely in plants and are closely related to plant resistance to heavy metals (HMs). Glutathione (GSH) and nonprotein thiols (NPTs) are the major components of PCs. This study investigates the role of the PCs of Cladophora rupestris in Pb 2+ accumulation and detoxification. The distribution of Pb 2+ in the PCs of C. rupestris was studied. FTIR and XPS are used to characterize the chelating power of Pb 2+ with PCs in C. rupestris. The curve fitting of the secondary protein structure is used to identify the functional groups with Pb 2+ . Results showed that the content of Pb 2+ in the PCs of C. rupestris increased with an increase in Pb 2+ stress. Pb content increased to 352 and 314 mg/kg in NPTs and GSH, respectively, when Pb stress concentration reached 7.5 mg/dm 3 . The Pb 2+ fraction of C. rupestris PCs reached a maximum of 10.8 and 9.3% in NPTs and GSH, respectively. The Pb 2+ uptake by GSH and NPTs was 40-48% and 52-60%, respectively. Pb 2+ bound with the PCs of C. rupestris, forming

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Robust adsorption of ellagic acid on galena surface for efficient flotation separation of molybdenite and galena

Tang,

Zhang,

Zeng

et al. 2025

Applied Surface Science

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XPS and NMR analyze the combined forms of Pb in Cladophora rupestris subcells and its detoxification

Cited by 12 publications

References 61 publications

Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Phytochelatins of Cladophora rupestris in Pb2+ absorption and its detoxification

Robust adsorption of ellagic acid on galena surface for efficient flotation separation of molybdenite and galena

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