Xuhuaishuia manganoxidans gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province

Wang, Long; Liu, Yan; Shi, Xiaochong; Wang, Yanan; Zheng, Yanfen; Dai, Xiaofeng; Zhang, Xiaohua

doi:10.1099/ijsem.0.000912

Cited by 21 publications

(3 citation statements)

References 33 publications

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“…Among these, the genus Brevirhabdus was predominant in TW2 ( Fig. 2c ), and the predominant species of this genus, B. pacific (see Table S5), has been reported to oxidize manganese ( 40 ). When the manganese oxides formed adhere to the surfaces of stainless steel pipes, they create an electric potential difference, thus exacerbating corrosion ( 41 ).…”

Section: Resultsmentioning

confidence: 99%

Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station

Warashina,

Sato,

Hinai

et al. 2024

Appl Environ Microbiol

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A major incident occurred at the Fukushima Daiichi Nuclear Power Station following the tsunami triggered by the Tohoku–Pacific Ocean Earthquake in March 2011, whereby seawater entered the torus room in the basement of the reactor building. Here, we identify and analyze the bacterial communities in the torus room water and several environmental samples. Samples of the torus room water (1 × 10 9 Bq 137 Cs/L) were collected by the Tokyo Electric Power Company Holdings from two sampling points between 30 cm and 1 m from the bottom of the room (TW1) and the bottom layer (TW2). A structural analysis of the bacterial communities based on 16S rRNA amplicon sequencing revealed that the predominant bacterial genera in TW1 and TW2 were similar. TW1 primarily contained the genus Limnobacter , a thiosulfate-oxidizing bacterium. γ-Irradiation tests on Limnobacter thiooxidans , the most closely related phylogenetically found in TW1, indicated that its radiation resistance was similar to ordinary bacteria. TW2 predominantly contained the genus Brevirhabdus , a manganese-oxidizing bacterium. Although bacterial diversity in the torus room water was lower than seawater near Fukushima, ~70% of identified genera were associated with metal corrosion. Latent environment allocation—an analytical technique that estimates habitat distributions and co-detection analyses—revealed that the microbial communities in the torus room water originated from a distinct blend of natural marine microbial and artificial bacterial communities typical of biofilms, sludge, and wastewater. Understanding the specific bacteria linked to metal corrosion in damaged plants is important for advancing decommissioning efforts. IMPORTANCE In the context of nuclear power station decommissioning, the proliferation of microorganisms within the reactor and piping systems constitutes a formidable challenge. Therefore, the identification of microbial communities in such environments is of paramount importance. In the aftermath of the Fukushima Daiichi Nuclear Power Station accident, microbial community analysis was conducted on environmental samples collected mainly outside the site. However, analyses using samples from on-site areas, including adjacent soil and seawater, were not performed. This study represents the first comprehensive analysis of microbial communities, utilizing meta 16S amplicon sequencing, with a focus on environmental samples collected from the radioactive element-containing water in the torus room, including the surrounding environments. Some of the identified microbial genera are shared with those previously identified in spent nuclear fuel pools in countries such as France and Brazil. Moreover, our discussion in this paper elucidates the correlation of many of these bacteria with metal corrosion.

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Section: Resultsmentioning

confidence: 99%

Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station

Warashina,

Sato,

Hinai

et al. 2024

Appl Environ Microbiol

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“…Using the feature classifier in QIIME2, filtered and chimaera-free sequences were aligned and clustered into operational taxonomic units (OTUs), at a >97% similarity level against the SILVA138 database ( Table S3 ) [ 44 ]. 16S rRNA gene data of known Mn-oxidizing bacteria were compiled from previous studies [ 2 , 3 , 4 , 8 , 9 , 10 , 25 , 26 , 39 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 ] ( Table S2 ).…”

Section: Methodsmentioning

confidence: 99%

Mineralogical and Genomic Constraints on the Origin of Microbial Mn Oxide Formation in Complexed Microbial Community at the Terrestrial Hot Spring

Tsukamoto

Kakegawa

2022

Life

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Manganese (Mn) oxides are widespread on the surface environments of the modern Earth. The role of microbial activities in the formation of Mn oxides has been discussed for several decades. However, the mechanisms of microbial Mn oxidation, and its role in complex microbial communities in natural environments, remain uncertain. Here, we report the geochemical, mineralogical, and metagenomic evidence for biogenic Mn oxides, found in Japanese hot spring sinters. The low crystallinity of Mn oxides, and their spatial associations with organic matter, support the biogenic origin of Mn oxides. Specific multicopper oxidases (MCOs), which are considered Mn-oxidizing enzymes, were identified using metagenomic analyses. Nanoscale nuggets of copper sulfides were, also, discovered in the organic matter in Mn-rich sinters. A part of these copper sulfides most likely represents traces of MCOs, and this is the first report of traces of Mn-oxidizing enzyme in geological samples. Metagenomic analyses, surprisingly, indicated a close association of Mn oxides, not only in aerobic but also in anaerobic microbial communities. These new findings offer the unique and unified positions of Mn oxides, with roles that have not been ignored, to sustain anaerobic microbial communities in hot spring environments.

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“…Species of the family Rhodobacteraceae within the class Alphaproteobacteria (Garrity et al, 2005) have most often been isolated from a wide range of marine environments (Iwaki et al, 2013;Park et al, 2014;Zhang et al 2015;Wang et al, 2016). At the time of writing, the family comprises 105 genera with validly published names (http:// www.bacterio.net/rhodobacteraceae.html).…”

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confidence: 99%

Cribrihabitans pelagius sp. nov., a marine alphaproteobacterium isolated from seawater

Yoon

Lee

2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, strictly aerobic, cream-coloured, motile, chemoheterotrophic, rod-shaped bacterium, designated strain KMU-32 T , was isolated from seawater at Najeong Beach in Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Rhodobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.2 %) to Cribrihabitans neustonicus CC-AMHB-3 T . The DNA-DNA relatedness value between strain KMU-32 T and C. neustonicus CC-AMHB-3 T was 48.5 ± 4 %. The DNA G+C content of strain KMU-32 T was determined to be 63.7 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acid was C 18 : 1 !7c. Strain KMU-32 T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, strain KMU-32 T is considered to represent a novel species of the genus Cribrihabitans, for which the name Cribrihabitans pelagius sp. nov. is proposed. The type strain is KMU-32 T (= KCTC 42981 T = NBRC 111834 T ).

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Xuhuaishuia manganoxidans gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province

Cited by 21 publications

References 33 publications

Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station

Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station

Mineralogical and Genomic Constraints on the Origin of Microbial Mn Oxide Formation in Complexed Microbial Community at the Terrestrial Hot Spring

Cribrihabitans pelagius sp. nov., a marine alphaproteobacterium isolated from seawater

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