Xylan synthesized byIrregular Xylem 14(IRX14) maintains the structure of seed coat mucilage inArabidopsis

Abstract

HighlightXylan synthesized by IRX14 maintains seed coat mucilage structure potentially through altering the crystallization and organization of cellulose.

UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage

“…The absence of UUAT1 also results in a reduction in Xyl in SM. However, this phenotype is different from the one observed for muci21 and irx14, xylosyltransferases mutants that show alterations in epidermal cell mucilage adhesion (Hu et al, 2016;Voiniciuc et al, 2015;Ralet et al, 2016). On the other hand, no Xyl (A) The uuat1-2 mutant displays early stem elongation when compared with the wild-type Col-0.…”

Effective extraction of Arabidopsis adherent seed mucilage by ultrasonic treatment

“…Fourier Transform Infrared Spectroscopy (FTIR) is an informative diagnostic tool to detect mucilage structures based on signature peaks481415. An analysis of the water-soluble non-adherent mucilage and ultrasonic treatment extracted adherent mucilage was performed by FTIR spectroscopy (Fig.…”

“…In contrast, the adherent mucilage is strongly adherent to the seed coat and cannot be removed by agitation, enzymatic digestion or harsh chemical treatments567. The adherent mucilage is primarily comprised of RG I, but also contains cellulose, galactan, xylan, arabinan and homogalacturonan (HG)458.…”

Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition inPlantagospp.

“…Upon contact with aqueous environments, the mucilage-producing cells rupture and mucilage is extruded, possibly aiding in seed dispersal and hydration during germination (Yang et al , 2012). Although the majority of plants found in arid sandy soils are myxospermous (Deng et al , 2012), the precise function of mucilage remains unknown, since mutants that lack the ability to extrude mucilage are still viable (Penfield et al , 2001; Huang et al , 2011). In addition, seed mucilage composition varies considerably across different genera.…”