Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

Goyal, Meenakshi; Kalra, K; Sareen, V. K.; Soni, G.

doi:10.1590/s1517-83822008000300025

Cited by 64 publications

(34 citation statements)

References 10 publications

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“…The enzyme was cellulase-free as the crude extract was devoid of cellulase activity. Lignocellulosic materials have been used earlier by various researchers for production of xylanase but with lower activity (3,9,12).…”

Section: Enzyme Activitymentioning

confidence: 99%

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH.Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular mass of 23 kDa. It showed an optimum pH at 7.0 and was stable over the pH range 6.0-9.0. The optimum temperature for enzyme activity was 55 °C. The purified xylanase did not lose any activity up to 45 ºC, however, it retained 80% and 51% of its activity after pre-incubation at 55 ºC and 60 ºC, respectively. The enzyme obeyed Michaelis-Menton kinetics towards birch wood xylan with apparent K m 3.33 mg/ml and V max 100 IU/ml. The enzyme was strongly inhibited by Hg 2+ and Cu 2+ while enhanced by Co 2+ and Mn 2+. The purified enzyme could be stored at 4 ºC for six weeks without any loss of catalytic activity. The faster and economical purification of the cellulase-free xylanase from B. subtilis ASH by onestep procedure together with its appreciable stability at high temperature and alkaline pH makes it potentially effective for industrial applications.

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Section: Enzyme Activitymentioning

confidence: 99%

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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“…At present, the dominant cost element in fermentative production of fuel ethanol is starch, though some fermentable starch and sucrose are easily obtainable from agricultural by-products such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw (12,19,36). Expansion of this fermentation process towards utilization of low-value substrates such as agro-industrial wastes offers a great potential for reducing the production cost and increasing the use of ethanol as a fuel additive.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Agro-industrial Wastes for the Simultaneous Production of Amylase and Xylanase by Thermophilic Actinomycetes

Singh¹,

Kapoor²,

Kumar³

2012

Braz. J. Microbiol.

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Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom compost. Among of these, strain designated MSC702 having high potential to utilize agro-industrial wastes for the production of amylase and xylanase. Strain MSC702 was identified as novel species of Streptomyces through morphological characterization and 16S rRNA gene sequence. Enzyme production was determined using 1% (w/v) of various agro-industrial waste in production medium containing (g/100mL): K 2 HPO 4 (0.1), (NH 4 ) 2 SO 4 (0.1), NaCl (0.1), MgSO 4 (0.1) at pH 7.0 after incubation of 48 h at 50°C. The amylase activity (373.89 IU/mL) and xylanase activity (30.15 IU/mL) was maximum in rice bran. The decreasing order of amylase and xylanase activity in different type of agro-industrial wastes were found rice bran (RB) > corn cob (CC) > wheat bran (WB) > wheat straw (WS) > sugarcane bagasse (SB) and rice bran (RB) > wheat bran (WB) > wheat straw (WS) > sugarcane bagasse (SB) > corn cob (CC), respectively. Mixed effect of different agroindustrial wastes was examined in different ratios. Enzyme yield of amylase and xylanase was ~1.3 and ~2.0 fold higher with RB: WB in 1:2 ratio.

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“…26,27 The optimum temperature for lignocellulolytic enzyme production was similar to those of other mesophilic fungi such as Aspergillus japonicas C03, Aspergillus glaucas XC8, A. niger MS82, Trichoderma reesei Rut C30, Trichoderma viride strain EU2-77, Penicillum echinulatum and Fusarium oxysporum. The maximum xylanase production has already been reported at a temperature range of [25][26][27][28][29][30] C by Trichoderma viride 28 and P. glabrum. 29 Usually, the maximal reported temperature for these filamentous fungus is 35 C, with an indication of the absence of growth 37 C. 30,31 The activity of lignocellulolytic enzymes also increased on increasing the agitation up to 100 rpm.…”

Section: Resultsmentioning

confidence: 84%

“…The enzyme production was carried out at different pH (4-6) when incubated at a different temp. (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) C) for 24-120 h at 50 ¡150 rpm based on the Central Composite Design (CCD) of RSM (Table 4 and Table 1). An aliquot of fermentation broth was withdrawn from the flask after a regular interval and centrifuged at 4 C at 7000 rpm for 15 min.…”

Section: Growth Conditions and Enzyme Production Set Upmentioning

confidence: 99%

Bioprocessing of wheat bran for the production of lignocellulolytic enzyme cocktail byCotylidia pannosaunder submerged conditions

2016

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Characterization and production of efficient lignocellulytic enzyme cocktails for biomass conversion is the need for biofuel industry. The present investigation reports the modeling and optimization studies of lignocellulolytic enzyme cocktail production by Cotylidia pannosa under submerged conditions. The predominant enzyme activities of cellulase, xylanase and laccase were produced in the cocktail through submerged conditions using wheat bran as a substrate. A central composite design approach was utilized to model the production process using temperature, pH, incubation time and agitation as input variables with the goal of optimizing the output variables namely cellulase, xylanase and laccase activities. The effect of individual, square and interaction terms on cellulase, xylanase and laccase activities were depicted through the non-linear regression equations with significant R 2 and P-values. An optimized value of 20 U/ml, 17 U/ml and 13 U/ml of cellulase, xylanase and laccase activities, respectively, were obtained with a media pH of 5.0 in 77 h at 31C, 140 rpm using wheatbran as a substrate. Overall, the present study introduces a fungal strain, capable of producing lignocellulolytic enzyme cocktail for subsequent applications in biofuel industry.

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Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

Cited by 64 publications

References 10 publications

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Utilization of Agro-industrial Wastes for the Simultaneous Production of Amylase and Xylanase by Thermophilic Actinomycetes

Bioprocessing of wheat bran for the production of lignocellulolytic enzyme cocktail byCotylidia pannosaunder submerged conditions

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