2021
DOI: 10.1016/j.pbi.2021.102135
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Xylem vessel cell differentiation: A best model for new integrative cell biology?

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Cited by 13 publications
(9 citation statements)
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“…PttXCP1 and PtMYB0003 were upregulated only in the PtCesA18 pro::AtSND1:VP16 plants (Figure 3), while the other SCW biosynthesis genes were upregulated in both the PtCesA18pro::AtMYB46:VP16 and PtCesA18pro::AtSND1:VP16 lines, relative to their expression in the non-transgenic plants and the vector control PtCesA18pro::GUS plants (Figure 3). This is consistent with the known transcriptional regulatory hierarchy, in which the VNS proteins, including AtSND1, can induce the expression of the secondary master switch MYB genes such as AtMYB46 and AtMYB83, as well as the PCD-related and SCW-related genes, while the secondary master switch MYB proteins mainly upregulate SCW-related genes (Zhong et al, 2010a;Schuetz et al, 2013;Nakano et al, 2015;Ohtani and Demura, 2019;Kamon and Ohtani, 2021). We could not confirm the increased abundance of the proteins of AtVND7:VP16, AtSND1:VP16, nor AtMYB46:VP16 proteins, due to the absence of specific antibodies for these transcription factors.…”
Section: Generation Of Transgenic Plants Containing the Self-reinforc...supporting
confidence: 87%
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“…PttXCP1 and PtMYB0003 were upregulated only in the PtCesA18 pro::AtSND1:VP16 plants (Figure 3), while the other SCW biosynthesis genes were upregulated in both the PtCesA18pro::AtMYB46:VP16 and PtCesA18pro::AtSND1:VP16 lines, relative to their expression in the non-transgenic plants and the vector control PtCesA18pro::GUS plants (Figure 3). This is consistent with the known transcriptional regulatory hierarchy, in which the VNS proteins, including AtSND1, can induce the expression of the secondary master switch MYB genes such as AtMYB46 and AtMYB83, as well as the PCD-related and SCW-related genes, while the secondary master switch MYB proteins mainly upregulate SCW-related genes (Zhong et al, 2010a;Schuetz et al, 2013;Nakano et al, 2015;Ohtani and Demura, 2019;Kamon and Ohtani, 2021). We could not confirm the increased abundance of the proteins of AtVND7:VP16, AtSND1:VP16, nor AtMYB46:VP16 proteins, due to the absence of specific antibodies for these transcription factors.…”
Section: Generation Of Transgenic Plants Containing the Self-reinforc...supporting
confidence: 87%
“…In our self-reinforced system, the transcription factors associated with SCW formation are expressed under the control of the PtCesA18 promoter, as a fusion protein with the human herpes virus–derived transcriptional activation domain VP16 ( Figure 1E ). The transcription factor genes used in the present work were AtSND1 , AtVND7 , and AtMYB46 , which are master regulators of SCW formation in A. thaliana ( Kubo et al, 2005 ; Mitsuda et al, 2005 , 2007 ; Zhong et al, 2006 , 2007 ; Ko et al, 2009 , 2014 ; Nakano et al, 2015 ; Ohtani and Demura, 2019 ; Kamon and Ohtani, 2021 ). The key feature of this self-reinforced system is that the PtCesA18 promoter can be upregulated by AtSND1, AtVND7, and AtMYB46, since these transcription factors can recognize and bind their cis-regulatory elements, which are highly conserved among each ortholog ( Zhong et al, 2010a ; Ohtani et al, 2011 ; Kim et al, 2012 ; Zhong and Ye, 2012 ).…”
Section: Resultsmentioning
confidence: 99%
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“…Comparing the electrophoretic patterns (Figure 2), we observed a clear difference in the band patterns between DMSO and DEX treatments in both the CaCl 2 and LiCl buffer samples, regardless of the cell wall extraction protocol: High‐molecular‐weight bands (>100 kDa) were detected only in the DMSO‐treated samples, but not in the DEX‐treated samples, whereas there were more low‐molecular‐weight bands (<37 kDa) in the DEX‐treated samples than in the DMSO‐treated samples. Programmed cell death with proteolysis is a feature of xylem vessel cell differentiation (Fukuda, 1997; Kamon & Ohtani, 2021; Turner et al., 2007); therefore, we presumed that these changes in protein size were related to the progression of proteolysis. Notably, the band patterns clearly differed between the CaCl 2 and LiCl buffer samples and among different cell wall extraction protocols (Figure 2).…”
Section: Resultsmentioning
confidence: 99%
“…Xylem vessels are important tissues in vascular plants that transport water and minerals. During their differentiation, xylem vessel cells develop thickened secondary cell walls (SCWs) composed of cellulose, hemicellulose, and lignin, and eventually undergo programmed cell death (PCD), resulting in a hollow structure (Turner et al, 2007;Kamon and Ohtani, 2021). Advances in molecular biological research have revealed much about the molecular mechanisms of xylem vessel cell differentiation; a factor facilitating this research was the development of an artificial induction system for xylem vessel cell differentiation (Tan et al, 2019).…”
Section: Introductionmentioning
confidence: 99%