Xylooligosaccharides: manufacture and applications

Vázquez, María José Domínguez; Alonso, José Luís; Domı́nguez, Herminia; Parajó, Juan Carlos

doi:10.1016/s0924-2244(01)00031-0

Cited by 595 publications

(303 citation statements)

References 17 publications

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“…XOS are oligomers of 2-10 xylose residues linked together by β (1-4) linkage which are produced by the hydrolysis of xylan, the major component of plant hemicelluloses either by autohydrolysis or by chemical treatment/enzymatic hydrolysis. Corncobs, hardwoods (birchwood and larchwood), straws, bagasse, rice hulls, malt cakes and bran serve as starting materials for XOS production (Vázquez et al 2000). XOS have been enzymatically produced from wheat bran alkali extractable hemicelluloses (Reddy and Krishnan 2010) and wheat bran water soluble polysaccharides (Madhukumar and Muralikrishna 2010).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant property of synbiotic combination of Lactobacillus sp. and wheat bran xylo-oligosaccharides

Lasrado

Muralikrishna

2014

J Food Sci Technol

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Wheat bran water unextractable portion (WB-WUP) was subjected to xylanase treatment to obtain a mixture of xylo-oligosaccharides (XOS). XOS mixture was purified on charcoal-celite column and the individual oligosaccharides were separated on a Bio-Gel P-2 column. The sugar composition of the purified oligosaccharides was determined by GLC and their structure was deduced by ESI-MS and 1 H and 13 C NMR. The major oligosaccharides identified were xylobiose and xylotriose (consisting of arabinose). Five strains of lactobacilli (probiotics), XOS (prebiotics) and a combination of both (synbiotics) in milk (as medium) were monitored for antioxidant activity. DPPH radical scavenging activity (~70 %) as well as ferric reducing power (~80 mg/ 100 ml FeSO 4 eq) were significantly higher (p<0.05) in all the synbiotic preparations compared to that of control. The present study indicated that the synbiotic preparations consisting of XOS and lactobacilli can be effectively used as dietary supplement.

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Section: Introductionmentioning

confidence: 99%

Antioxidant property of synbiotic combination of Lactobacillus sp. and wheat bran xylo-oligosaccharides

Lasrado

Muralikrishna

2014

J Food Sci Technol

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“…2 A recent and exciting application of endoxylanases is the production of xylo-oligosaccharides with huge commercial value, because xylooligosaccharides, especially xylobiose, have been found to have a stimulatory effect on the selective growth of human intestinal Bifidobacteria, and are frequently defined as prebiotics. 3,4 Another economically important application of Xyl-11 deals with animal feeding. For instance, in poultry production, xylanase supplementation in diet affects growth performance, digestion, and impact on immune parameters and gut microflora.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of His-tagged recombinant xylanase fromPenicillium occitanison Nickel-chelate Eupergit C for increasing digestibility of poultry feed

Driss

Châari

et al. 2014

Bioengineered

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Characterization of the immobilized PoXyn2 was further evaluated. The optimum pH was not affected by immobilization, but the immobilized PoXyn2 exhibited more acidic and large optimum pH range (pH 2.0-4.0) than that of the free PoXyn2 (pH 3.0). The free PoXyn2 had an optimum temperature of 50 °C, whereas that of the immobilized enzyme was shifted to 65 °C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Thermodynamically, increase in enthalpy and free energy change after covalent immobilization could be credited to the enhanced stability. Immobilized xylanase could be reused for 10 consecutive cycles retaining 60% of its initial activity. It was found to be effective in releasing reducing sugar from poultry feed. Immobilization on Eupergit C is important due to its mechanical resistance at high pH and temperature. Hence, considerable stability and reusability of bound enzyme may be advantageous for its industrial application.

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“…Xylo-oligosacharides products have wide applications in industries, such as in juice-processing and textile industries, bread industry [7]. In poultry industries, they are added in feed processing to improve the feed digestions and nutrients uptake [3,8,9].…”

Section: Introductionmentioning

confidence: 99%

Cloning and Over-expression of xynB Gene of Bacillus subtilis subsp. spizizenii W23 into Escherichia coli Origami Host Cells

Wahjudi

Catherina²,

Wangunhardjo³

et al. 2017

KLS

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The xynB gene of Bacillus subtilis subsp. spizizenii W23 is predicted to encode a xylan 1,4-beta-xylosidase. Application of XynB enzymes in industries is wide. Production of this enzyme in its host cells is naturally restricted by repression process. It will give certain beneficial to over-expressed the enzymes in other host-cells under inducing promoter. This study aimed to clone the xynB gene from Bacillus subtilis subsp. spizizenii W23, to pMMB67EH plasmid, and to over-express the xynB gene in Escherichia coli Origami as host cells. The xynB gene was successfully amplified by polymerase chain reaction (PCR) technique using a pair of primers flanking the gene sequence and chromosomal DNA of the W23 strain as a template. The xynB gene inserted in recombinant plasmid was confirmed by PCR detection using primers pair's specific for xynB gene and for the vector, then continued by restriction analyses. The result showed that transformants clone 9 and 10 bear the recombinant pMMB-xynB plasmid. The xylanase activity of xynB gene in Escherichia coli Origami clone 10 was detected by sodium-dodecyl-sulfate polyacrylamide gel analyses and with addition of isopropyl--D-thio-galactoside (IPTG) as an inducer. The protein seem to be overexpressed as intra-and extra-cellular protein detected on SDS-PAGE gel. Result from xylan degrading activity on Luria-Bertani-xylan-IPTG plate with addition of Congo Red, showed that the cells with pMMB-xynB recombinant plasmid have clear zone around the colonies while the transformant bearing an empty plasmid showed no clear zone. It could be concluded that the xynB gene of Bacillus subtilis subsp.spizizenii W23 has been successfully been cloned on pMMB67EH plasmid and over-expressed in the Escherichia coli Origami cells as intra-and extra-cellular protein, as observed on SDS-PAGE gel analysis. The protein has activity on xylan degradation.

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Xylooligosaccharides: manufacture and applications

Cited by 595 publications

References 17 publications

Antioxidant property of synbiotic combination of Lactobacillus sp. and wheat bran xylo-oligosaccharides

Antioxidant property of synbiotic combination of Lactobacillus sp. and wheat bran xylo-oligosaccharides

Immobilization of His-tagged recombinant xylanase fromPenicillium occitanison Nickel-chelate Eupergit C for increasing digestibility of poultry feed

Cloning and Over-expression of xynB Gene of Bacillus subtilis subsp. spizizenii W23 into Escherichia coli Origami Host Cells

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