Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum

Mindt, Melanie; Heuser, Maria; Wendisch, Volker F.

doi:10.1016/j.biortech.2019.02.084

Cited by 37 publications

(39 citation statements)

References 46 publications

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“…Changing the substrate can force C. glutamicum to produce metabolites with different concentrations . To realize the type and concentration, production time is important for substrate adjusting and high efficiency production, especially in fermentation industry . AAs are a class of important metabolites of C. glutamicum .…”

Section: Resultsmentioning

confidence: 99%

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

et al. 2019

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Corynebacterium glutamicum (C. glutamicum) is a well‐known workhorse for the industrial production of amino acids. Different carbon, nitrogen, and sulfur source may force the bacterium to produce specific metabolites. In this work, a method of high‐speed MEKC with LIF detection was developed to rapidly analyze the amino acid metabolites released by C. glutamicum, which is fed with different culture mediums. Corynebacterium glutamicum was cultured in microbial fuel cells to monitor its metabolism process and collect its metabolites. In the CE system, a microliter‐scale sample reservoir was designed and applied to perform tiny volume sample injection. With the assistance of microwave, the derivatization time for amino acids with FITC was greatly shortened to 6 min. Under the optimized condition, the eight candidate amino acids of metabolites could be rapidly separated within 2 min. The whole analysis process for real samples, from sampling to determination, could be shortened to less than 10 min. The results showed that C. glutamicum could produce additional l‐lysine and l‐valine as the metabolites when fed with glucose and l‐methionine, respectively. The method proved that culture mediums used to feed C. glutamicum had great effect on the bacterium's metabolites.

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Section: Resultsmentioning

confidence: 99%

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

et al. 2019

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“…For example, anthranilate N-methylation described here as well as N-methylglutamate production established in Pseudomonas putida using N-methylglutamate synthase and γ-glutamylmethylamide synthetase of the methylamine assimilation pathway of Methylobacterium extorquens [13] have narrow substrate spectra (e.g., GMAS from Methylovorus mays also forms γ-glutamylethylamide, also known as theanine [69]) compared with N-alkylation using the imine reductase DpkA of Pseudomonas putida [12]. Several methylated or ethylated amino acids could be produced by C. glutamicum using the wild-type or a mutant version of DpkA and either MMA or ethylamine as substrates [14,34,35]. With respect to aromatic amino acids, N-methyl-l-phenylalanine could be obtained from phenylpyruvate by enzyme catalysis using DpkA and MMA [12]; however, production of NMA via DpkA by N-alkylamination of a carbonyl precursor of NMA has not been described.…”

Section: Discussionmentioning

confidence: 99%

“…A well-established toolbox enabled metabolic engineered-based approaches for production of diverse value-added compounds. Besides the production of proteinogenic amino acids, also a broad range of non-proteinogenic amino acid products like γ-aminobutyrate [ 29 ], 5-aminovalerate [ 30 , 31 ], pipecolic acid [ 32 , 33 ], N -methylated amino acids like N -methylalanine (NMeAla) [ 34 ] and sarcosine [ 35 ], aromatic compounds like 4-hydroxybenzoate [ 36 , 37 ] or protocatechuic acid [ 38 ], and functionalized aromatics like 7-chloro- or 7-bromo-tryptophan [ 39 , 40 ] and O -methylanthranilate [ 41 ] have been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Fermentative N-Methylanthranilate Production by Engineered Corynebacterium glutamicum

et al. 2020

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The N-functionalized amino acid N-methylanthranilate is an important precursor for bioactive compounds such as anticancer acridone alkaloids, the antinociceptive alkaloid O-isopropyl N-methylanthranilate, the flavor compound O-methyl-N-methylanthranilate, and as a building block for peptide-based drugs. Current chemical and biocatalytic synthetic routes to N-alkylated amino acids are often unprofitable and restricted to low yields or high costs through cofactor regeneration systems. Amino acid fermentation processes using the Gram-positive bacterium Corynebacterium glutamicum are operated industrially at the million tons per annum scale. Fermentative processes using C. glutamicum for N-alkylated amino acids based on an imine reductase have been developed, while N-alkylation of the aromatic amino acid anthranilate with S-adenosyl methionine as methyl-donor has not been described for this bacterium. After metabolic engineering for enhanced supply of anthranilate by channeling carbon flux into the shikimate pathway, preventing by-product formation and enhancing sugar uptake, heterologous expression of the gene anmt encoding anthranilate N-methyltransferase from Ruta graveolens resulted in production of N-methylanthranilate (NMA), which accumulated in the culture medium. Increased SAM regeneration by coexpression of the homologous adenosylhomocysteinase gene sahH improved N-methylanthranilate production. In a test bioreactor culture, the metabolically engineered C. glutamicum C1* strain produced NMA to a final titer of 0.5 g·L−1 with a volumetric productivity of 0.01 g·L−1·h−1 and a yield of 4.8 mg·g−1 glucose.

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“…Xylose is the second most abundant sustainable raw material for fermentation. Xylose is obtained from the hydrolysis of lignocellulosic biomasses and is widely used for the production of valuable chemicals, such as succinate (Mao et al, 2018), sarcosine (Mindt et al, 2019), 5-aminovalerate (Jorge et al, 2017b), L-pipecolic acid (Perez-Garcia et al, 2017), 3-hydroxypropionic acid (Chen et al, 2017), and γ-aminobutyric acid (Jorge et al, 2017a) in engineered C. glutamicum strains. The utilization of xylose can be improved by heterologous expression of xylose isomerase (Jo et al, 2017), overexpression of myo-inositol/proton symporter IolT1 (Brusseler et al, 2018), and adaptive laboratory evolution (Radek et al, 2017) of C. glutamicum.…”

Section: Alternative Substratesmentioning

confidence: 99%

“…Arabinose is another component of lignocellulosic biomass hydrolysate and is widely used for producing valuable compounds (Zahoor et al, 2012;Zhao et al, 2018). Several studies have developed superior microbial cell factories, such as C. glutamicum that can efficiently utilize arabinose (Henke et al, 2018;Kawaguchi et al, 2018;Mindt et al, 2019). The heterologous expression of araBAD operon derived from E. coli enabled the C. glutamicum ORN1 strain to utilize arabinose.…”

Section: Alternative Substratesmentioning

confidence: 99%

Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum

Guo

Zhang

et al. 2020

Front. Bioeng. Biotechnol.

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L-ornithine, a valuable non-protein amino acid, has a wide range of applications in the pharmaceutical and food industries. Currently, microbial fermentation is a promising, sustainable, and environment-friendly method to produce L-ornithine. However, the industrial production capacity of L-ornithine by microbial fermentation is low and rarely meets the market demands. Various strategies have been employed to improve the L-ornithine production titers in the model strain, Corynebacterium glutamicum, which serves as a major indicator for improving the cost-effectiveness of L-ornithine production by microbial fermentation. This review focuses on the development of high L-ornithine-producing strains by metabolic engineering and reviews the recent advances in breeding strategies, such as reducing by-product formation, improving the supplementation of precursor glutamate, releasing negative regulation and negative feedback inhibition, increasing the supply of intracellular cofactors, modulating the central metabolic pathway, enhancing the transport system, and adaptive evolution for improving L-ornithine production.

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Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum

Cited by 37 publications

References 46 publications

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

Fermentative N-Methylanthranilate Production by Engineered Corynebacterium glutamicum

Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum

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