Y A-T-Il En Belgique Un Accroissement De Resistance D’Escherichia Coli Considere Comme Bacterie De Reference Vis-A-Vis De L’Association Amoxicilline/Acide Clavulanique?

Yourassowsky, E; Linden, Van der; Gb, MacGilavry; Glupczynsky, Y.

doi:10.1080/17843286.1992.11718205

Cited by 4 publications

(2 citation statements)

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“…This probably explains the wide range of frequencies of resistance to amoxicillin-clavulanic acid that have been reported for amoxicillinresistant E. coli, including 3% for Spanish isolates collected from 1983 to 1988 (23), 10% for isolates collected in Belgium (38), 28% for isolates collected in Spain (8), 33% for strains collected in 1993 in Clermont-Ferrand, France (10), 35% for American strains collected in 1992 (12), and 41% for strains collected in Hong Kong from 1984 to 1988 (14). In the present study, we found about 11% of amoxicillin-resistant strains to be resistant to the combination of amoxicillin and clavulanic acid by disk diffusion tests, but 71% of these were subsequently shown to be moderately susceptible rather than resistant on the basis of MICs.…”

Section: Discussionmentioning

confidence: 97%

“…It has been noted that difficulties encountered in measuring susceptibility to amoxicillin-clavulanic acid can cause disagreements between laboratories and that evaluation of increased resistance is specific for each laboratory (38). This probably explains the wide range of frequencies of resistance to amoxicillin-clavulanic acid that have been reported for amoxicillinresistant E. coli, including 3% for Spanish isolates collected from 1983 to 1988 (23), 10% for isolates collected in Belgium (38), 28% for isolates collected in Spain (8), 33% for strains collected in 1993 in Clermont-Ferrand, France (10), 35% for American strains collected in 1992 (12), and 41% for strains collected in Hong Kong from 1984 to 1988 (14).…”

Section: Discussionmentioning

confidence: 99%

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Incidence and mechanisms of resistance to the combination of amoxicillin and clavulanic acid in Escherichia coli

Stapleton¹,

Wu²,

King³

et al. 1995

Antimicrob Agents Chemother

137

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Among Escherichia coli organisms isolated at St. Thomas's Hospital during the years 1990 to 1994, the frequency of resistance to amoxicillin-clavulanic acid (tested by disk diffusion in a ratio of 2:1) remained constant at about 5% of patient isolates (10 to 15% of the 41 to 45% that were amoxicillin resistant). Mechanisms of increased resistance were determined for 72 consecutively collected such amoxicillin-clavulanic acid-resistant isolates. MICs of the combination were 16-8 g/ml for 51 (71%) of these and >32-16 g/ml for the remainder. The predominant mechanism was hyperproduction of enzymes isoelectrically cofocusing with TEM-1 (␤-lactamase activities, >200 nmol of nitrocefin hydrolyzed per min per mg of protein) which was found in 44 isolates (61%); two isolates produced smaller amounts (approximately 150 nmol/min/mg) of such enzymes, and two isolates hyperproduced enzymes cofocusing with TEM-2. Eleven isolates produced enzymes cofocusing with OXA-1 ␤-lactamase, which has previously been associated with resistance to amoxicillinclavulanic acid. Ten isolates produced increased amounts of chromosomal ␤-lactamase, and four of these additionally produced TEM-1 or TEM-2. Three isolates produced inhibitor-resistant TEM-group enzymes. In one of the enzymes (pI, 5.4), the amino acid sequence change was Met-673Val, and thus the enzyme is identical to TEM-34. Another (pI, 5.4) had the substitution Met-673Ile and is identical to IRT-I67, which we propose now be given the designation TEM-40. The third (pI, 5.2) had the substitution Arg-2413Thr; this enzyme has not been reported previously and should be called TEM-41. The rarity and diversity of inhibitor-resistant TEM-group enzymes suggest that they are the result of spontaneous mutations that have not yet spread.

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Section: Discussionmentioning

confidence: 97%