YajL, the Prokaryotic Homolog of the Parkinsonism-Associated Protein DJ-1, Protects Cells against Protein Sulfenylation

Gautier, Valérie; Le, Hai-Tuong; Malki, Abderrahim; Messaoudi, Nadia; Caldas, Teresa; Kthiri, Fatoum; Landoulsi, Ahmed; Richarme, Gilbert

doi:10.1016/j.jmb.2012.01.047

Cited by 24 publications

(32 citation statements)

References 43 publications

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“…Overexpression of trigger factor in the yajL mutant may be linked to its ribosomal and translation accuracy defects (44). The clear results showing overexpression of chaperones and proteases in the yajL mutant contrast with contradictory results obtained in Parkinson's cells (24,25,45) and are in accordance with the protein aggregation phenotype of this mutant (9,20,21). Osmotic-stress proteins and chemical chaperones.…”

Section: Resultsmentioning

confidence: 80%

“…Since unfolded proteins act as intracellular signals for induction of the heat shock response (42,43), the overexpression of chaperones, proteases, and peptidases in the yajL mutant likely results from its protein oxidation and aggregation defects (9,20) and is probably mediated by the overexpression of sigma 32, the main regulator of the E. coli unfolded-protein response (see below). Overexpression of trigger factor in the yajL mutant may be linked to its ribosomal and translation accuracy defects (44).…”

Section: Resultsmentioning

confidence: 99%

“…The overexpression of sigma S in the yajL mutant (see below) likely mediates the upregulation of several osmotic-stress genes (proP, proVWX, otsA, otsB, and osmC are controlled by sigma S [54]). The upregulation of genes coding for chemical chaperones and the activation of hydrophobic compound efflux systems in the yajL mutant suggests that this mutant uses a variety of mechanisms to alleviate its protein aggregation defect (9,20,21).…”

Section: Resultsmentioning

confidence: 99%

“…Protein aggregates mainly contain lone subunits of multiprotein complexes, such as those of ribosomes and ATP synthase. In relation to their function in oxidative-stress protection, YajL, and DJ-1 expressed in Escherichia coli, function as covalent chaperones that, upon oxidative stress, form mixed disulfides with sulfenylated proteins of the thiol proteome, including chaperones, proteases, ribosomal proteins, catalases, peroxidases, and FeS proteins (20,21); accordingly, two major FeS proteins, aconitase B and NADH dehydrogenase I, were almost inactive in the yajL mutant, and both YajL-and DJ-1-overproducing plasmids rescued them (21).…”

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confidence: 99%

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Global Stress Response in a Prokaryotic Model of DJ-1-Associated Parkinsonism

Messaoudi¹,

Gautier²,

Kthiri³

et al. 2013

Journal of Bacteriology

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YajL is the most closely related Escherichia coli homolog of Parkinsonism-associated protein DJ-1, a protein with a yet-undefined function in the oxidative-stress response. YajL protects cells against oxidative-stress-induced protein aggregation and functions as a covalent chaperone for the thiol proteome, including FeS proteins. To clarify the cellular responses to YajL deficiency, transcriptional profiling of the yajL mutant was performed. Compared to the parental strain, the yajL mutant overexpressed genes coding for chaperones, proteases, chemical chaperone transporters, superoxide dismutases, catalases, peroxidases, components of thioredoxin and glutaredoxin systems, iron transporters, ferritins and FeS cluster biogenesis enzymes, DNA repair proteins, RNA chaperones, and small regulatory RNAs. It also overexpressed the RNA polymerase stress sigma factors sigma S (multiple stresses) and sigma 32 (protein stress) and activated the OxyR and SoxRS oxidative-stress transcriptional regulators, which together trigger the global stress response. The yajL mutant also overexpressed genes involved in septation and adopted a shorter and rounder shape characteristic of stressed bacteria. Biochemical experiments showed that this upregulation of many stress genes resulted in increased expression of stress proteins and improved biochemical function. Thus, protein defects resulting from the yajL mutation trigger the onset of a robust and global stress response in a prokaryotic model of DJ-1-associated Parkinsonism.

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Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Global Stress Response in a Prokaryotic Model of DJ-1-Associated Parkinsonism

Messaoudi¹,

Gautier²,

Kthiri³

et al. 2013

Journal of Bacteriology

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“…4A). Deglycase DJ-1 repairs methylglyoxal-or glyoxal-glycated (Richarme et al, 2015) and sulfenylated proteins (Gautier et al, 2012) and defends cells against oxidative damage (Wilson, 2011). The decrease in DJ-1 abundance is counterintuitive; however, it is possible that the change in expression represents a PTM, with a concomitant increase in abundance in another spot on the 2D gel that was not detected as significantly increasing in abundance.…”

Section: Protein Unfolding and Oxidative Stress Protectionmentioning

confidence: 99%

Rapid proteomic responses to a near-lethal heat stress in the salt marsh musselGeukensia demissa

Fields

Burmester

Cox

et al. 2016

Journal of Experimental Biology

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Acute heat stress perturbs cellular function on a variety of levels, leading to protein dysfunction and aggregation, oxidative stress and loss of metabolic homeostasis. If these challenges are not overcome quickly, the stressed organism can die. To better understand the earliest tissue-level responses to heat stress, we examined the proteomic response of gill from Geukensia demissa, an extremely eurythermal mussel from the temperate intertidal zone of eastern North America. We exposed 15°C-acclimated individuals to an acute near-lethal heat stress (45°C) for 1 h, and collected gill samples from 0 to 24 h of recovery. The changes in protein expression we found reveal a coordinated physiological response to acute heat stress: proteins associated with apoptotic processes were increased in abundance during the stress itself (i.e. at 0 h of recovery), while protein chaperones and foldases increased in abundance soon after (3 h). The greatest number of proteins changed abundance at 6 h; these included oxidative stress proteins and enzymes of energy metabolism. Proteins associated with the cytoskeleton and extracellular matrix also changed in abundance starting at 6 h, providing evidence of cell proliferation, migration and tissue remodeling. By 12 h, the response to acute heat stress was diminishing, with fewer stress and structural proteins changing in abundance. Finally, the proteins with altered abundances identified at 24 h suggest a return to the pre-stress anabolic state.

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Advanced Photoacoustic Imaging Applications of Near‐Infrared Absorbing Organic Nanoparticles

Jiang

2017

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261

180

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Progress of nanotechnology in recent years has stimulated fast development of nanoparticles in biomedical research. Photoacoustic (PA) imaging as an emerging non-invasive technique in molecular imaging has improved imaging depth relative to conventional optical imaging, demonstrating great potential in clinical applications. The convergence of nanotechnology and PA imaging has enabled a broad spectrum of new opportunities in fundamental biology and translation medicine. This review focuses on the recent advances of organic nanoparticles in PA imaging applications. Near-infrared absorbing organic nanoparticles are classified and discussed according to their different imaging applications, which include tumor imaging, gastrointestinal imaging, sentinel lymph node imaging, disease microenvironment imaging and real-time drug imaging. The chemistry and PA properties of organic nanoparticles are discussed in details to highlight their own merits, and their challenges and perspectives in PA imaging are also discussed.

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YajL, the Prokaryotic Homolog of the Parkinsonism-Associated Protein DJ-1, Protects Cells against Protein Sulfenylation

Cited by 24 publications

References 43 publications

Global Stress Response in a Prokaryotic Model of DJ-1-Associated Parkinsonism

Global Stress Response in a Prokaryotic Model of DJ-1-Associated Parkinsonism

Rapid proteomic responses to a near-lethal heat stress in the salt marsh musselGeukensia demissa

Advanced Photoacoustic Imaging Applications of Near‐Infrared Absorbing Organic Nanoparticles

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